Abstract
Electrophysiologic properties of cultured human keratinocytes were studied using the patch voltage-clamp technique. Undifferentiated, proliferate keratinocytes grown in low Ca2+ medium had an average resting membrane potential of -24 mV. Voltage-clamp experiments showed that these cells had two membrane ionic currents: a large voltage-independent leak conductance, and a smaller voltage-dependent Cl- current that activated with depolarization. Increasing the extracellular Ca2+ concentration from 0.15 to 2 mM resulted in a doubling of the magnitude of the voltage-gated current and a shift in current activation to more negative potentials. Since levels of extracellular Ca2+ can alter the morphology and differentiation state of keratinocytes, the finding of a Ca2+-activated Cl- current in these cells suggests a role for this conductance in the initiation of differentiation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 13-20 |
| Number of pages | 8 |
| Journal | Journal of Cellular Physiology |
| Volume | 143 |
| Issue number | 1 |
| State | Published - 1990 |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
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Extracellular calcium affects the membrane currents of cultured human keratinocytes. / Mauro, Theodora M.; Pappone, Pamela A.; Isseroff, Roslyn Rivkah.
In: Journal of Cellular Physiology, Vol. 143, No. 1, 1990, p. 13-20.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Extracellular calcium affects the membrane currents of cultured human keratinocytes
AU - Mauro, Theodora M.
AU - Pappone, Pamela A.
AU - Isseroff, Roslyn Rivkah
PY - 1990
Y1 - 1990
N2 - Electrophysiologic properties of cultured human keratinocytes were studied using the patch voltage-clamp technique. Undifferentiated, proliferate keratinocytes grown in low Ca2+ medium had an average resting membrane potential of -24 mV. Voltage-clamp experiments showed that these cells had two membrane ionic currents: a large voltage-independent leak conductance, and a smaller voltage-dependent Cl- current that activated with depolarization. Increasing the extracellular Ca2+ concentration from 0.15 to 2 mM resulted in a doubling of the magnitude of the voltage-gated current and a shift in current activation to more negative potentials. Since levels of extracellular Ca2+ can alter the morphology and differentiation state of keratinocytes, the finding of a Ca2+-activated Cl- current in these cells suggests a role for this conductance in the initiation of differentiation.
AB - Electrophysiologic properties of cultured human keratinocytes were studied using the patch voltage-clamp technique. Undifferentiated, proliferate keratinocytes grown in low Ca2+ medium had an average resting membrane potential of -24 mV. Voltage-clamp experiments showed that these cells had two membrane ionic currents: a large voltage-independent leak conductance, and a smaller voltage-dependent Cl- current that activated with depolarization. Increasing the extracellular Ca2+ concentration from 0.15 to 2 mM resulted in a doubling of the magnitude of the voltage-gated current and a shift in current activation to more negative potentials. Since levels of extracellular Ca2+ can alter the morphology and differentiation state of keratinocytes, the finding of a Ca2+-activated Cl- current in these cells suggests a role for this conductance in the initiation of differentiation.
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M3 - Article
C2 - 1690740
AN - SCOPUS:0025275464
VL - 143
SP - 13
EP - 20
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 1
ER -