Extracellular bone matrix-derived growth factor

T. K. Sampath, D. P. DeSimone, A Hari Reddi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Demineralized extracellular bone matrix, when implanted subcutaneously into allogeneic rats, induces an invariant sequence of events resulting in de novo cartilage, bone and bone marrow formation. We have recently demonstrated the dissociative extraction and successful biological reconstitution of boneinducing molecule(s) in demineralized bone matrix. As mesenchymal cell proliferation precedes differentiation of endochondral bone, we have examined the bone-inductive molecules for mitogenic activity on human and rat fibroblasts and bovine endothelial cells. The results revealed that the molecular fraction obtained by molecular sieve chromatography on Sepharose CL-6B responsible for endochondral bone induction is capable of stimulating human and rat fibroblasts proliferation by 2500 and 300 % respectively, as compared to cells grown in a serum-free medium. Endothelial cells do not seem to share this response. This factor exhibits a significant effect on growth-promoting activity for both human and rat fibroblasts at a dose of 0.5 ng protein/ml of culture medium. These results demonstrate the presence of a tightly bound growth factor in the extracellular bone matrix.

Original languageEnglish (US)
Pages (from-to)460-464
Number of pages5
JournalExperimental Cell Research
Volume142
Issue number2
DOIs
StatePublished - 1982
Externally publishedYes

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Bone Matrix
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Bone and Bones
Fibroblasts
Endothelial Cells
Serum-Free Culture Media
Osteogenesis
Human Activities
Cartilage
Gel Chromatography
Culture Media
Bone Marrow
Cell Proliferation
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Extracellular bone matrix-derived growth factor. / Sampath, T. K.; DeSimone, D. P.; Reddi, A Hari.

In: Experimental Cell Research, Vol. 142, No. 2, 1982, p. 460-464.

Research output: Contribution to journalArticle

Sampath, T. K. ; DeSimone, D. P. ; Reddi, A Hari. / Extracellular bone matrix-derived growth factor. In: Experimental Cell Research. 1982 ; Vol. 142, No. 2. pp. 460-464.
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