Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome

Young S. Lee, Catherine A. Vandevoort, John P. Gaughan, Uros Midic, Zoran Obradovic, Keith E. Latham

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The elaboration of a quality oocyte is integrally linked to the correct developmental progression of cumulus cell phenotype. In humans and nonhuman primates, oocyte quality is diminished with in vitro maturation. To determine the changes in gene expression in rhesus monkey cumulus cells (CC) that occur during the final day prior to oocyte maturation and how these changes differ between in vitro (IVM) and in vivo maturation (VVM), we completed a detailed comparison of transcriptomes using the Affymetrix gene array. We observed a large number of genes differing in expression when comparing IVM-CC and VVM-CC directly but a much larger number of differences when comparing the transitions from the prematuration to the post-IVM and post-VVM states. We observed a truncation or delay in the normal pattern of gene regulation but also remarkable compensatory changes in gene expression during IVM. Among the genes affected by IVM are those that contribute to productive cell-cell interactions between cumulus cell and oocyte and between cumulus cells. Numerous genes involved in lipid metabolism are incorrectly regulated during IVM, and the synthesis of sex hormones appears not to be suppressed during IVM. We identified a panel of 24 marker genes, the expression of which should provide the foundation for understanding how IVM can be improved for monitoring IVM conditions and for diagnosing oocyte quality.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume301
Issue number1
DOIs
StatePublished - Jul 2011

Fingerprint

In Vitro Oocyte Maturation Techniques
Cumulus Cells
Macaca mulatta
Transcriptome
Oocytes
Genes
Gene Expression
Gonadal Steroid Hormones
Lipid Metabolism
Cell Communication
Primates
Phenotype

Keywords

  • In vitro maturation
  • Oocyte quality

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Endocrinology, Diabetes and Metabolism

Cite this

Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome. / Lee, Young S.; Vandevoort, Catherine A.; Gaughan, John P.; Midic, Uros; Obradovic, Zoran; Latham, Keith E.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 301, No. 1, 07.2011.

Research output: Contribution to journalArticle

Lee, Young S. ; Vandevoort, Catherine A. ; Gaughan, John P. ; Midic, Uros ; Obradovic, Zoran ; Latham, Keith E. / Extensive effects of in vitro oocyte maturation on rhesus monkey cumulus cell transcriptome. In: American Journal of Physiology - Endocrinology and Metabolism. 2011 ; Vol. 301, No. 1.
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