Extension of the four-parameter logistic model for ELISA to multianalyte analysis

Geoffrey Jones, Monika Wortberg, Sabine B. Kreissig, David S. Bunch, Shirley J. Gee, Bruce D. Hammock, David M Rocke

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


The standard implementation of enzyme-linked immunosorbent assay (ELISA) for single analytes can lead to false conclusions if cross reacting compounds are present in the sample. This paper discusses the extension of the usual four-parameter logistic model for ELISA to the case of multiple cross-reacting analytes. The use of the extended model in multianalyte analysis (MELISA) is illustrated and compared with a more simplistic approach. Data on the analysis of a binary mixture of s-triazines suggests the superiority of the proposed model. This model is also suitable for other forms of assay that use the four-parameter logistic curve.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalJournal of Immunological Methods
Issue number1-2
StatePublished - Dec 28 1994


  • Cross-reactivity
  • Immunoassay
  • Logistic model

ASJC Scopus subject areas

  • Biotechnology
  • Immunology


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