Expression profiling of immune-related genes from Japanese flounder Paralichthys olivaceus kidney cells using cDNA microarrays

Tomofumi Kurobe, Motoshige Yasuike, Takuto Kimura, Ikuo Hirono, Takashi Aoki

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

A Japanese flounder Paralichthys olivaceus cDNA microarray containing 871 unique cDNAs including 91 putative immune-related genes from our EST studies was constructed and used to characterize of gene expression of in vitro grown kidney cells stimulated with mitogens such as ConA, PMA, LPS or infected with hirame rhabdovirus (HRV). The numbers of genes whose expressions were increased or decreased by these factors were: 17 by Con A, 139 by PMA, 76 by LPS and 182 by HRV infection. The treatment of Con A for 1 and 6 h affected the expression of only a few of the immune-related genes. PMA down-regulated far more genes than it up-regulated. Apoptosis-related factors, such as c-fos, NGF induced protein IB and NR13 genes, were among the genes whose expressions were induced by PMA. LPS induced the expression of inflammation-related genes, such as IL-1β, monocyte chemotactic protein 1 and collagenase. The expressions of many genes were induced after 3 h HRV infection but some of them were decreased to the basal level after 6 h HRV infection. The expression of some genes of unknown function were induced or reduced by Con A, PMA or LPS or by HRV infection in different time periods. From all of the gene expression profiling in this study, we could get lots of information about the dynamic changes in the gene expression of the kidney cells under different stress or stimulations.

Original languageEnglish (US)
Pages (from-to)515-523
Number of pages9
JournalDevelopmental and Comparative Immunology
Volume29
Issue number6
DOIs
StatePublished - Jan 1 2005
Externally publishedYes

Fingerprint

Novirhabdovirus
Flounder
Oligonucleotide Array Sequence Analysis
Kidney
Gene Expression
Genes
Infection
Chemokine CCL2
Expressed Sequence Tags
Gene Expression Profiling
Nerve Growth Factor
Collagenases
Interleukin-1
Mitogens
Complementary DNA
Apoptosis
Inflammation
Proteins

Keywords

  • cDNA microarray
  • Concanavalin A. Phorbol myristate acetate
  • Hirame rhabdovirus
  • Japanese flounder
  • Kidney cells
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Expression profiling of immune-related genes from Japanese flounder Paralichthys olivaceus kidney cells using cDNA microarrays. / Kurobe, Tomofumi; Yasuike, Motoshige; Kimura, Takuto; Hirono, Ikuo; Aoki, Takashi.

In: Developmental and Comparative Immunology, Vol. 29, No. 6, 01.01.2005, p. 515-523.

Research output: Contribution to journalArticle

@article{9712e6ddd3f9417395ac09d8af822747,
title = "Expression profiling of immune-related genes from Japanese flounder Paralichthys olivaceus kidney cells using cDNA microarrays",
abstract = "A Japanese flounder Paralichthys olivaceus cDNA microarray containing 871 unique cDNAs including 91 putative immune-related genes from our EST studies was constructed and used to characterize of gene expression of in vitro grown kidney cells stimulated with mitogens such as ConA, PMA, LPS or infected with hirame rhabdovirus (HRV). The numbers of genes whose expressions were increased or decreased by these factors were: 17 by Con A, 139 by PMA, 76 by LPS and 182 by HRV infection. The treatment of Con A for 1 and 6 h affected the expression of only a few of the immune-related genes. PMA down-regulated far more genes than it up-regulated. Apoptosis-related factors, such as c-fos, NGF induced protein IB and NR13 genes, were among the genes whose expressions were induced by PMA. LPS induced the expression of inflammation-related genes, such as IL-1β, monocyte chemotactic protein 1 and collagenase. The expressions of many genes were induced after 3 h HRV infection but some of them were decreased to the basal level after 6 h HRV infection. The expression of some genes of unknown function were induced or reduced by Con A, PMA or LPS or by HRV infection in different time periods. From all of the gene expression profiling in this study, we could get lots of information about the dynamic changes in the gene expression of the kidney cells under different stress or stimulations.",
keywords = "cDNA microarray, Concanavalin A. Phorbol myristate acetate, Hirame rhabdovirus, Japanese flounder, Kidney cells, Lipopolysaccharide",
author = "Tomofumi Kurobe and Motoshige Yasuike and Takuto Kimura and Ikuo Hirono and Takashi Aoki",
year = "2005",
month = "1",
day = "1",
doi = "10.1016/j.dci.2004.10.005",
language = "English (US)",
volume = "29",
pages = "515--523",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Expression profiling of immune-related genes from Japanese flounder Paralichthys olivaceus kidney cells using cDNA microarrays

AU - Kurobe, Tomofumi

AU - Yasuike, Motoshige

AU - Kimura, Takuto

AU - Hirono, Ikuo

AU - Aoki, Takashi

PY - 2005/1/1

Y1 - 2005/1/1

N2 - A Japanese flounder Paralichthys olivaceus cDNA microarray containing 871 unique cDNAs including 91 putative immune-related genes from our EST studies was constructed and used to characterize of gene expression of in vitro grown kidney cells stimulated with mitogens such as ConA, PMA, LPS or infected with hirame rhabdovirus (HRV). The numbers of genes whose expressions were increased or decreased by these factors were: 17 by Con A, 139 by PMA, 76 by LPS and 182 by HRV infection. The treatment of Con A for 1 and 6 h affected the expression of only a few of the immune-related genes. PMA down-regulated far more genes than it up-regulated. Apoptosis-related factors, such as c-fos, NGF induced protein IB and NR13 genes, were among the genes whose expressions were induced by PMA. LPS induced the expression of inflammation-related genes, such as IL-1β, monocyte chemotactic protein 1 and collagenase. The expressions of many genes were induced after 3 h HRV infection but some of them were decreased to the basal level after 6 h HRV infection. The expression of some genes of unknown function were induced or reduced by Con A, PMA or LPS or by HRV infection in different time periods. From all of the gene expression profiling in this study, we could get lots of information about the dynamic changes in the gene expression of the kidney cells under different stress or stimulations.

AB - A Japanese flounder Paralichthys olivaceus cDNA microarray containing 871 unique cDNAs including 91 putative immune-related genes from our EST studies was constructed and used to characterize of gene expression of in vitro grown kidney cells stimulated with mitogens such as ConA, PMA, LPS or infected with hirame rhabdovirus (HRV). The numbers of genes whose expressions were increased or decreased by these factors were: 17 by Con A, 139 by PMA, 76 by LPS and 182 by HRV infection. The treatment of Con A for 1 and 6 h affected the expression of only a few of the immune-related genes. PMA down-regulated far more genes than it up-regulated. Apoptosis-related factors, such as c-fos, NGF induced protein IB and NR13 genes, were among the genes whose expressions were induced by PMA. LPS induced the expression of inflammation-related genes, such as IL-1β, monocyte chemotactic protein 1 and collagenase. The expressions of many genes were induced after 3 h HRV infection but some of them were decreased to the basal level after 6 h HRV infection. The expression of some genes of unknown function were induced or reduced by Con A, PMA or LPS or by HRV infection in different time periods. From all of the gene expression profiling in this study, we could get lots of information about the dynamic changes in the gene expression of the kidney cells under different stress or stimulations.

KW - cDNA microarray

KW - Concanavalin A. Phorbol myristate acetate

KW - Hirame rhabdovirus

KW - Japanese flounder

KW - Kidney cells

KW - Lipopolysaccharide

UR - http://www.scopus.com/inward/record.url?scp=14744280589&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14744280589&partnerID=8YFLogxK

U2 - 10.1016/j.dci.2004.10.005

DO - 10.1016/j.dci.2004.10.005

M3 - Article

VL - 29

SP - 515

EP - 523

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 6

ER -