Expression patterns of the E2F family of transcription factors during murine epithelial development

L. Dagnino, C. J. Fry, S. M. Bartley, P. Farnham, B. L. Gallie, R. A. Phillips

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The E2F family of transcription factors includes five E2F and three DP forms. E2F is involved in the regulation of cell proliferation, but little is known about E2F function during vertebrate development. We have explored the regulation of E2F expression during mouse organogenesis by in situ hybridization. We find selective up-regulation of E2F-2, E2F-4, and E2F-5 transcripts in epidermis and intestinal epithelium at important developmental stages. E2F-4 transcript levels are high in early, undifferentiated single- cell-layer ectoderm, and later in 13.5-14.5-day-postcoitus (dpc) embryo epithelium, which contains several layers of proliferating cells. E2F-2 is up-regulated following the onset of E2F-4 expression and is first apparent in undifferentiated epithelium at 13.5-14.5 days of gestation. In contrast, E2F- 5 transcripts are detected later in gestation, once the epidermis shows evidence of stratification. Stratification of the epidermis into basal, proliferating cells and suprabasal, terminally differentiating cells at 15.5- 19.5 days of gestation coincides with expression of E2F-2 and E2F-4 in basal cells and of E2F-5 in suprabasal cells. Similarly, in intestinal epithelium, E2F-4 up-regulation in pseudostratified epithelium at 13.5 days of gestation precedes appearance of E2F-2 transcripts, in 14.5-dpc embryos, in the proliferating, intervillus epithelium. In 16.5-19.5-dpc embryos, no E2F-2 transcripts were detected at the tip of the developing villi, which contain terminally differentiating cells. In contrast, E2F-5 transcripts were limited to the upper half of the villi and were absent in the intervillus epithelium. This suggests that E2F-2 and E2F-4 may participate in maintaining epithelial cells in a proliferative, undifferentiated phenotype, whereas E2F-5 may be important to maintain the differentiated state. Thus, selective regulation of E2F forms occurs during murine epithelial development, irrespective of the ectodermal or endodermal origin of such epithelia.

Original languageEnglish (US)
Pages (from-to)553-563
Number of pages11
JournalCell Growth and Differentiation
Volume8
Issue number5
StatePublished - May 1997
Externally publishedYes

Fingerprint

E2F Transcription Factors
Epithelium
Epidermis
Pregnancy
Embryonic Structures
Intestinal Mucosa
Up-Regulation
Ectoderm
Organogenesis
In Situ Hybridization
Vertebrates
Epithelial Cells
Cell Proliferation
Phenotype

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Dagnino, L., Fry, C. J., Bartley, S. M., Farnham, P., Gallie, B. L., & Phillips, R. A. (1997). Expression patterns of the E2F family of transcription factors during murine epithelial development. Cell Growth and Differentiation, 8(5), 553-563.

Expression patterns of the E2F family of transcription factors during murine epithelial development. / Dagnino, L.; Fry, C. J.; Bartley, S. M.; Farnham, P.; Gallie, B. L.; Phillips, R. A.

In: Cell Growth and Differentiation, Vol. 8, No. 5, 05.1997, p. 553-563.

Research output: Contribution to journalArticle

Dagnino, L, Fry, CJ, Bartley, SM, Farnham, P, Gallie, BL & Phillips, RA 1997, 'Expression patterns of the E2F family of transcription factors during murine epithelial development', Cell Growth and Differentiation, vol. 8, no. 5, pp. 553-563.
Dagnino L, Fry CJ, Bartley SM, Farnham P, Gallie BL, Phillips RA. Expression patterns of the E2F family of transcription factors during murine epithelial development. Cell Growth and Differentiation. 1997 May;8(5):553-563.
Dagnino, L. ; Fry, C. J. ; Bartley, S. M. ; Farnham, P. ; Gallie, B. L. ; Phillips, R. A. / Expression patterns of the E2F family of transcription factors during murine epithelial development. In: Cell Growth and Differentiation. 1997 ; Vol. 8, No. 5. pp. 553-563.
@article{84779299a91e4db494aa4c7e5fac7de4,
title = "Expression patterns of the E2F family of transcription factors during murine epithelial development",
abstract = "The E2F family of transcription factors includes five E2F and three DP forms. E2F is involved in the regulation of cell proliferation, but little is known about E2F function during vertebrate development. We have explored the regulation of E2F expression during mouse organogenesis by in situ hybridization. We find selective up-regulation of E2F-2, E2F-4, and E2F-5 transcripts in epidermis and intestinal epithelium at important developmental stages. E2F-4 transcript levels are high in early, undifferentiated single- cell-layer ectoderm, and later in 13.5-14.5-day-postcoitus (dpc) embryo epithelium, which contains several layers of proliferating cells. E2F-2 is up-regulated following the onset of E2F-4 expression and is first apparent in undifferentiated epithelium at 13.5-14.5 days of gestation. In contrast, E2F- 5 transcripts are detected later in gestation, once the epidermis shows evidence of stratification. Stratification of the epidermis into basal, proliferating cells and suprabasal, terminally differentiating cells at 15.5- 19.5 days of gestation coincides with expression of E2F-2 and E2F-4 in basal cells and of E2F-5 in suprabasal cells. Similarly, in intestinal epithelium, E2F-4 up-regulation in pseudostratified epithelium at 13.5 days of gestation precedes appearance of E2F-2 transcripts, in 14.5-dpc embryos, in the proliferating, intervillus epithelium. In 16.5-19.5-dpc embryos, no E2F-2 transcripts were detected at the tip of the developing villi, which contain terminally differentiating cells. In contrast, E2F-5 transcripts were limited to the upper half of the villi and were absent in the intervillus epithelium. This suggests that E2F-2 and E2F-4 may participate in maintaining epithelial cells in a proliferative, undifferentiated phenotype, whereas E2F-5 may be important to maintain the differentiated state. Thus, selective regulation of E2F forms occurs during murine epithelial development, irrespective of the ectodermal or endodermal origin of such epithelia.",
author = "L. Dagnino and Fry, {C. J.} and Bartley, {S. M.} and P. Farnham and Gallie, {B. L.} and Phillips, {R. A.}",
year = "1997",
month = "5",
language = "English (US)",
volume = "8",
pages = "553--563",
journal = "Molecular Cancer Research",
issn = "1541-7786",
publisher = "American Association for Cancer Research Inc.",
number = "5",

}

TY - JOUR

T1 - Expression patterns of the E2F family of transcription factors during murine epithelial development

AU - Dagnino, L.

AU - Fry, C. J.

AU - Bartley, S. M.

AU - Farnham, P.

AU - Gallie, B. L.

AU - Phillips, R. A.

PY - 1997/5

Y1 - 1997/5

N2 - The E2F family of transcription factors includes five E2F and three DP forms. E2F is involved in the regulation of cell proliferation, but little is known about E2F function during vertebrate development. We have explored the regulation of E2F expression during mouse organogenesis by in situ hybridization. We find selective up-regulation of E2F-2, E2F-4, and E2F-5 transcripts in epidermis and intestinal epithelium at important developmental stages. E2F-4 transcript levels are high in early, undifferentiated single- cell-layer ectoderm, and later in 13.5-14.5-day-postcoitus (dpc) embryo epithelium, which contains several layers of proliferating cells. E2F-2 is up-regulated following the onset of E2F-4 expression and is first apparent in undifferentiated epithelium at 13.5-14.5 days of gestation. In contrast, E2F- 5 transcripts are detected later in gestation, once the epidermis shows evidence of stratification. Stratification of the epidermis into basal, proliferating cells and suprabasal, terminally differentiating cells at 15.5- 19.5 days of gestation coincides with expression of E2F-2 and E2F-4 in basal cells and of E2F-5 in suprabasal cells. Similarly, in intestinal epithelium, E2F-4 up-regulation in pseudostratified epithelium at 13.5 days of gestation precedes appearance of E2F-2 transcripts, in 14.5-dpc embryos, in the proliferating, intervillus epithelium. In 16.5-19.5-dpc embryos, no E2F-2 transcripts were detected at the tip of the developing villi, which contain terminally differentiating cells. In contrast, E2F-5 transcripts were limited to the upper half of the villi and were absent in the intervillus epithelium. This suggests that E2F-2 and E2F-4 may participate in maintaining epithelial cells in a proliferative, undifferentiated phenotype, whereas E2F-5 may be important to maintain the differentiated state. Thus, selective regulation of E2F forms occurs during murine epithelial development, irrespective of the ectodermal or endodermal origin of such epithelia.

AB - The E2F family of transcription factors includes five E2F and three DP forms. E2F is involved in the regulation of cell proliferation, but little is known about E2F function during vertebrate development. We have explored the regulation of E2F expression during mouse organogenesis by in situ hybridization. We find selective up-regulation of E2F-2, E2F-4, and E2F-5 transcripts in epidermis and intestinal epithelium at important developmental stages. E2F-4 transcript levels are high in early, undifferentiated single- cell-layer ectoderm, and later in 13.5-14.5-day-postcoitus (dpc) embryo epithelium, which contains several layers of proliferating cells. E2F-2 is up-regulated following the onset of E2F-4 expression and is first apparent in undifferentiated epithelium at 13.5-14.5 days of gestation. In contrast, E2F- 5 transcripts are detected later in gestation, once the epidermis shows evidence of stratification. Stratification of the epidermis into basal, proliferating cells and suprabasal, terminally differentiating cells at 15.5- 19.5 days of gestation coincides with expression of E2F-2 and E2F-4 in basal cells and of E2F-5 in suprabasal cells. Similarly, in intestinal epithelium, E2F-4 up-regulation in pseudostratified epithelium at 13.5 days of gestation precedes appearance of E2F-2 transcripts, in 14.5-dpc embryos, in the proliferating, intervillus epithelium. In 16.5-19.5-dpc embryos, no E2F-2 transcripts were detected at the tip of the developing villi, which contain terminally differentiating cells. In contrast, E2F-5 transcripts were limited to the upper half of the villi and were absent in the intervillus epithelium. This suggests that E2F-2 and E2F-4 may participate in maintaining epithelial cells in a proliferative, undifferentiated phenotype, whereas E2F-5 may be important to maintain the differentiated state. Thus, selective regulation of E2F forms occurs during murine epithelial development, irrespective of the ectodermal or endodermal origin of such epithelia.

UR - http://www.scopus.com/inward/record.url?scp=0030991285&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030991285&partnerID=8YFLogxK

M3 - Article

C2 - 9149906

AN - SCOPUS:0030991285

VL - 8

SP - 553

EP - 563

JO - Molecular Cancer Research

JF - Molecular Cancer Research

SN - 1541-7786

IS - 5

ER -