Abstract
Purpose. To determine whether human uveal melanoma cells can produce vascular endothelial growth factor (VEGF) and whether VEGF production can be modulated by a cytokine found in the uvea, namely transforming growth factor-beta (TGF-beta). Methods. Five different cultured human uveal melanoma cell lines of differing cellular morphology (2 spindle, 2 epithelioid, 1 mixed), obtained from different tumor sites (4 intraocular, 1 extrascleral/orbital) were used. Cells were grown in serum-free media for 24 hrs in the presence or absence of TGF-beta (1ng/ml). The conditioned media was harvested and assayed for VEGF using an immunoiluorometric technique. Results. All cultured human uveal melanoma cell lines produced VEGF. Among cells derived from intraocular tumor, TGF-beta stimulated VEGF production of epithelioid cells but had no effect on VEGF production of spindle cells. In contrast, TGF-beta inhibited VEGF production of tumor cells derived from an extrascleral orbital site. Conclusions. Cultured human uveal melanoma cells produce VEGF, and TGF-beta can have a variable effect of VEGF production of these cells.
| Original language | English (US) |
|---|---|
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 37 |
| Issue number | 3 |
| State | Published - Feb 15 1996 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Ophthalmology
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Expression of Vascular Endothelial Growth Factor (VEGF) by cultured human uveal melanoma cells : Effect of Transforming Growth Factor-beta (TGF-BETA). / Park, Susanna Soon Chun; Li, L.; Korn, T. S.; Yeo, K. T.
In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 3, 15.02.1996.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of Vascular Endothelial Growth Factor (VEGF) by cultured human uveal melanoma cells
T2 - Effect of Transforming Growth Factor-beta (TGF-BETA)
AU - Park, Susanna Soon Chun
AU - Li, L.
AU - Korn, T. S.
AU - Yeo, K. T.
PY - 1996/2/15
Y1 - 1996/2/15
N2 - Purpose. To determine whether human uveal melanoma cells can produce vascular endothelial growth factor (VEGF) and whether VEGF production can be modulated by a cytokine found in the uvea, namely transforming growth factor-beta (TGF-beta). Methods. Five different cultured human uveal melanoma cell lines of differing cellular morphology (2 spindle, 2 epithelioid, 1 mixed), obtained from different tumor sites (4 intraocular, 1 extrascleral/orbital) were used. Cells were grown in serum-free media for 24 hrs in the presence or absence of TGF-beta (1ng/ml). The conditioned media was harvested and assayed for VEGF using an immunoiluorometric technique. Results. All cultured human uveal melanoma cell lines produced VEGF. Among cells derived from intraocular tumor, TGF-beta stimulated VEGF production of epithelioid cells but had no effect on VEGF production of spindle cells. In contrast, TGF-beta inhibited VEGF production of tumor cells derived from an extrascleral orbital site. Conclusions. Cultured human uveal melanoma cells produce VEGF, and TGF-beta can have a variable effect of VEGF production of these cells.
AB - Purpose. To determine whether human uveal melanoma cells can produce vascular endothelial growth factor (VEGF) and whether VEGF production can be modulated by a cytokine found in the uvea, namely transforming growth factor-beta (TGF-beta). Methods. Five different cultured human uveal melanoma cell lines of differing cellular morphology (2 spindle, 2 epithelioid, 1 mixed), obtained from different tumor sites (4 intraocular, 1 extrascleral/orbital) were used. Cells were grown in serum-free media for 24 hrs in the presence or absence of TGF-beta (1ng/ml). The conditioned media was harvested and assayed for VEGF using an immunoiluorometric technique. Results. All cultured human uveal melanoma cell lines produced VEGF. Among cells derived from intraocular tumor, TGF-beta stimulated VEGF production of epithelioid cells but had no effect on VEGF production of spindle cells. In contrast, TGF-beta inhibited VEGF production of tumor cells derived from an extrascleral orbital site. Conclusions. Cultured human uveal melanoma cells produce VEGF, and TGF-beta can have a variable effect of VEGF production of these cells.
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M3 - Article
AN - SCOPUS:33750183956
VL - 37
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 3
ER -