Expression of Vascular Endothelial Growth Factor (VEGF) by cultured human uveal melanoma cells: Effect of Transforming Growth Factor-beta (TGF-BETA)

Susanna Soon Chun Park, L. Li, T. S. Korn, K. T. Yeo

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Abstract

Purpose. To determine whether human uveal melanoma cells can produce vascular endothelial growth factor (VEGF) and whether VEGF production can be modulated by a cytokine found in the uvea, namely transforming growth factor-beta (TGF-beta). Methods. Five different cultured human uveal melanoma cell lines of differing cellular morphology (2 spindle, 2 epithelioid, 1 mixed), obtained from different tumor sites (4 intraocular, 1 extrascleral/orbital) were used. Cells were grown in serum-free media for 24 hrs in the presence or absence of TGF-beta (1ng/ml). The conditioned media was harvested and assayed for VEGF using an immunoiluorometric technique. Results. All cultured human uveal melanoma cell lines produced VEGF. Among cells derived from intraocular tumor, TGF-beta stimulated VEGF production of epithelioid cells but had no effect on VEGF production of spindle cells. In contrast, TGF-beta inhibited VEGF production of tumor cells derived from an extrascleral orbital site. Conclusions. Cultured human uveal melanoma cells produce VEGF, and TGF-beta can have a variable effect of VEGF production of these cells.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

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Transforming Growth Factor beta
Vascular Endothelial Growth Factor A
Uvea
Uveal melanoma
human VEGFA protein
Cell Line
Epithelioid Cells
Neoplasms
Serum-Free Culture Media
Conditioned Culture Medium
Cytokines

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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title = "Expression of Vascular Endothelial Growth Factor (VEGF) by cultured human uveal melanoma cells: Effect of Transforming Growth Factor-beta (TGF-BETA)",
abstract = "Purpose. To determine whether human uveal melanoma cells can produce vascular endothelial growth factor (VEGF) and whether VEGF production can be modulated by a cytokine found in the uvea, namely transforming growth factor-beta (TGF-beta). Methods. Five different cultured human uveal melanoma cell lines of differing cellular morphology (2 spindle, 2 epithelioid, 1 mixed), obtained from different tumor sites (4 intraocular, 1 extrascleral/orbital) were used. Cells were grown in serum-free media for 24 hrs in the presence or absence of TGF-beta (1ng/ml). The conditioned media was harvested and assayed for VEGF using an immunoiluorometric technique. Results. All cultured human uveal melanoma cell lines produced VEGF. Among cells derived from intraocular tumor, TGF-beta stimulated VEGF production of epithelioid cells but had no effect on VEGF production of spindle cells. In contrast, TGF-beta inhibited VEGF production of tumor cells derived from an extrascleral orbital site. Conclusions. Cultured human uveal melanoma cells produce VEGF, and TGF-beta can have a variable effect of VEGF production of these cells.",
author = "Park, {Susanna Soon Chun} and L. Li and Korn, {T. S.} and Yeo, {K. T.}",
year = "1996",
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language = "English (US)",
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T1 - Expression of Vascular Endothelial Growth Factor (VEGF) by cultured human uveal melanoma cells

T2 - Effect of Transforming Growth Factor-beta (TGF-BETA)

AU - Park, Susanna Soon Chun

AU - Li, L.

AU - Korn, T. S.

AU - Yeo, K. T.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Purpose. To determine whether human uveal melanoma cells can produce vascular endothelial growth factor (VEGF) and whether VEGF production can be modulated by a cytokine found in the uvea, namely transforming growth factor-beta (TGF-beta). Methods. Five different cultured human uveal melanoma cell lines of differing cellular morphology (2 spindle, 2 epithelioid, 1 mixed), obtained from different tumor sites (4 intraocular, 1 extrascleral/orbital) were used. Cells were grown in serum-free media for 24 hrs in the presence or absence of TGF-beta (1ng/ml). The conditioned media was harvested and assayed for VEGF using an immunoiluorometric technique. Results. All cultured human uveal melanoma cell lines produced VEGF. Among cells derived from intraocular tumor, TGF-beta stimulated VEGF production of epithelioid cells but had no effect on VEGF production of spindle cells. In contrast, TGF-beta inhibited VEGF production of tumor cells derived from an extrascleral orbital site. Conclusions. Cultured human uveal melanoma cells produce VEGF, and TGF-beta can have a variable effect of VEGF production of these cells.

AB - Purpose. To determine whether human uveal melanoma cells can produce vascular endothelial growth factor (VEGF) and whether VEGF production can be modulated by a cytokine found in the uvea, namely transforming growth factor-beta (TGF-beta). Methods. Five different cultured human uveal melanoma cell lines of differing cellular morphology (2 spindle, 2 epithelioid, 1 mixed), obtained from different tumor sites (4 intraocular, 1 extrascleral/orbital) were used. Cells were grown in serum-free media for 24 hrs in the presence or absence of TGF-beta (1ng/ml). The conditioned media was harvested and assayed for VEGF using an immunoiluorometric technique. Results. All cultured human uveal melanoma cell lines produced VEGF. Among cells derived from intraocular tumor, TGF-beta stimulated VEGF production of epithelioid cells but had no effect on VEGF production of spindle cells. In contrast, TGF-beta inhibited VEGF production of tumor cells derived from an extrascleral orbital site. Conclusions. Cultured human uveal melanoma cells produce VEGF, and TGF-beta can have a variable effect of VEGF production of these cells.

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