Expression of Transforming Growth Factor β1 in Patients with and without Previous Abdominal Surgery

Kristina G. Hobson, Michelle DeWing, Hung S Ho, Bruce M. Wolfe, Kiho Cho, David G Greenhalgh

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Hypothesis: Transforming growth factor β1 (TGF-β1) plays an important role in the formation of adhesions after abdominal operations. Design: Prospective, observational study. Setting: University-based, tertiary referral center. Patients: Patients undergoing elective open abdominal operations were recruited and divided into 2 groups. Twenty-two patients with a history of abdominal surgery were designated as study patients, and 10 patients with no history of abdominal surgery served as controls. Interventions: Samples of normal peritoneum, peritoneal scar tissues, and serum were obtained from all patients at the time of surgery. Main Outcome Measures: Samples were assayed for total TGF-β1 expression using an enzyme-linked immunosorbent assay. Results: Scar tissues expressed significantly greater amounts of TGF-β1 (0.47 pg/μL) compared with normal peritoneal tissue from both study patients (0.29 pg/ μL; P=.03) and controls (0.17 pg/μL; P=.002). Serum TGF-β1 levels were also higher in study patients (1.71 pg/μL) compared with controls (1.22 pg/μL; P=.02). Neither adhesion nor serum TGF-β1 expression correlated with time since last operation, total number of previous operations, or severity of intra-abdominal adhesions. Conclusion: These results suggest that TGF-β1 may play an important role in human peritoneal adhesion formation.

Original languageEnglish (US)
Pages (from-to)1249-1252
Number of pages4
JournalArchives of Surgery
Volume138
Issue number11
DOIs
StatePublished - Nov 2003

ASJC Scopus subject areas

  • Surgery

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