Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis

David S. Goldfarb, Roy H. Doi, Raymond L. Rodriguez

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The ability of cells to discriminate between homologous and foreign genes manifests itself differently from one species to the next. Escherichia coli is well known for its ability to express genes from various donor species, including Bacillus subtilis1,2. In contrast, B. subtilis strongly discriminates against the expression of E. coli genes (there have been no corroborated reports of the expression of a wholly intact E. coli gene in B. subtilis) but expresses genes from other Gram-positive bacteria such as Staphylococcus aureus3. This apparent asymmetry in the expression of heterologous genes between E. coli and B. subtilis may reflect fundamental differences in the mechanisms of gene expression in these two model systems. Here, we have overcome the inability of B. subtilis to express E. coli chloramphenicol resistance (Cmr) by supplanting the native regulatory element(s) of this Tn9-derived gene with B. subtilis DNA fragments, which represents the first step towards dissecting the nature of this asymmetric barrier to gene expression.

Original languageEnglish (US)
Pages (from-to)309-311
Number of pages3
JournalNature
Volume293
Issue number5830
DOIs
StatePublished - 1981

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Chloramphenicol Resistance
Bacillus subtilis
Escherichia coli
Genes
Gene Expression
Gram-Positive Bacteria
Staphylococcus
Bacillus
DNA

ASJC Scopus subject areas

  • General

Cite this

Goldfarb, D. S., Doi, R. H., & Rodriguez, R. L. (1981). Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis. Nature, 293(5830), 309-311. https://doi.org/10.1038/293309a0

Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis. / Goldfarb, David S.; Doi, Roy H.; Rodriguez, Raymond L.

In: Nature, Vol. 293, No. 5830, 1981, p. 309-311.

Research output: Contribution to journalArticle

Goldfarb, DS, Doi, RH & Rodriguez, RL 1981, 'Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis', Nature, vol. 293, no. 5830, pp. 309-311. https://doi.org/10.1038/293309a0
Goldfarb, David S. ; Doi, Roy H. ; Rodriguez, Raymond L. / Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis. In: Nature. 1981 ; Vol. 293, No. 5830. pp. 309-311.
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