Expression of the RT6 mono(ADP-ribosyl)transferases is regulated by two promoter regions

Gregor Kuhlenbäumer, Stefan Rothenburg, Martina Matthes, Christiane Hollmann, Edgar Wingender, Heinz Günter Thiele, Friedrich Koch-Nolte, Friedrich Haag

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Abstract

The structure of the RT6 mono(ADP-ribosyl)transferase gene was studied. Analysis of cDNA clones revealed eight exons and suggested two independent transcriptional start sites. The existence of the downstream initiation site was confirmed by S1-nuclease protection and localized to position +29 of exon 2. The corresponding 5' flanking regions were found to contain typical promoter structures such as TATA- and CCAAT-boxes. Comparison with sequences deposited in the TRANSFAC database of transcription factor binding sites revealed few putative regulatory elements in the region associated with exon 1 (promoter 1). In contrast, several elements contained in the regulatory regions of other T cell-specific genes, such as ets, lyf-1 and ikaros were found in in promoter 2. Analysis of RT6-transcripts showed this region to be the most active promoter in spleen cells of adult rats. Finally, transient transfection assays with reporter gene constructs showed promoter 2 to mediate T-cell specific transcription.

Original languageEnglish (US)
Pages (from-to)265-270
Number of pages6
JournalAdvances in Experimental Medicine and Biology
Volume419
StatePublished - 1997
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kuhlenbäumer, G., Rothenburg, S., Matthes, M., Hollmann, C., Wingender, E., Thiele, H. G., Koch-Nolte, F., & Haag, F. (1997). Expression of the RT6 mono(ADP-ribosyl)transferases is regulated by two promoter regions. Advances in Experimental Medicine and Biology, 419, 265-270.