Expression of the MAS proto-oncogene in the retinal pigment epithelium of the rhesus macaque

Takashi Kitaoka, Mohammed Sharif, Michael R. Hanley, Leonard M Hjelmeland

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The MAS proto-oncogene codes for a seven transmembrane protein which has been previously localized to specific regions of the hippocampus and cerebral cortex in the rat central nervous system. Because MAS has biological properties related to the growth and differentiation of cells of neuroectodermal origin, we investigated the distribution of MAS expression in the rhesus macaque retina by in situ hybridization. A 330 base pair (bp) segment of the human MAS sequence was subcloned and used to generate single-stranded cRNA probes for these studies. Our results demonstrated little, if any, positive signal over the neurons of the retina. The use of epi-polarization microscopy, however, revealed a distinct positive labelling of retinal pigment epithelial (RPE) cells. These studies suggest the use of MAS as a possible marker for the retinal pigment epithelium.

Original languageEnglish (US)
Pages (from-to)345-351
Number of pages7
JournalCurrent Eye Research
Volume13
Issue number5
DOIs
StatePublished - 1994

Keywords

  • Epipolarization microscopy
  • In situ hybridization
  • MAS proto-oncogene
  • Retinal pigment epithelium (RPE)
  • Rhesus monkey

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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