Expression of the low-affinity NGF receptor during human muscle development, regeneration, and in tissue culture

P. Baron, E. Scarpini, G. Meola, I. Santilli, G. Conti, David E Pleasure, G. Scarlato

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The expression of the low-affinity NGF receptor (LNGFR) during human muscle development, regeneration, and in tissue culture was analyzed using a murine monoclonal antibody to human LNGFR (MAb ME 20.4). Muscle cells from 12-22 week fetuses stained strongly for LNGFR. In adult normal muscle, only intramuscular nerve endings showed immunoreactivity with MAb ME 20.4, but no staining was detected in muscle fibers. In Duchenne muscular dystrophy, immunohistologically demonstrable LNGFR was present in regenerating muscle fibers. In these fibers, LNGFR gene expression was also demonstrated at the transcriptional level using in situ hybridization with riboprobes coding with human LNGFR. Cultures from human fetal and adult muscle were studied by double label indirect immunofluorescence microscopy with MAb ME 20.4 and antisera against human fetal myosin. Most myosin-positive cells, both at the myoblast and myotube stages, displayed surface LNGFR immunostaining. In cells from fetal muscle, LNGFR was detected during the first 2 weeks in vitro, whereas in cells from adult muscle the expression of LNGFR was observed for up to 7 weeks. These findings suggest a potential involvement of LNGFR in human muscle development and regeneration.

Original languageEnglish (US)
Pages (from-to)276-284
Number of pages9
JournalMuscle and Nerve
Volume17
Issue number3
StatePublished - 1994
Externally publishedYes

Fingerprint

Nerve Growth Factor Receptor
Muscle Development
Human Development
Regeneration
Muscle Cells
Muscles
Myosins
Nerve Endings
Duchenne Muscular Dystrophy
Myoblasts
Skeletal Muscle Fibers
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
In Situ Hybridization
Immune Sera
Fetus
Monoclonal Antibodies
Staining and Labeling
Gene Expression

Keywords

  • DMD
  • human muscle
  • LNGFR
  • muscle cells
  • regenerating fibers

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Baron, P., Scarpini, E., Meola, G., Santilli, I., Conti, G., Pleasure, D. E., & Scarlato, G. (1994). Expression of the low-affinity NGF receptor during human muscle development, regeneration, and in tissue culture. Muscle and Nerve, 17(3), 276-284.

Expression of the low-affinity NGF receptor during human muscle development, regeneration, and in tissue culture. / Baron, P.; Scarpini, E.; Meola, G.; Santilli, I.; Conti, G.; Pleasure, David E; Scarlato, G.

In: Muscle and Nerve, Vol. 17, No. 3, 1994, p. 276-284.

Research output: Contribution to journalArticle

Baron, P, Scarpini, E, Meola, G, Santilli, I, Conti, G, Pleasure, DE & Scarlato, G 1994, 'Expression of the low-affinity NGF receptor during human muscle development, regeneration, and in tissue culture', Muscle and Nerve, vol. 17, no. 3, pp. 276-284.
Baron, P. ; Scarpini, E. ; Meola, G. ; Santilli, I. ; Conti, G. ; Pleasure, David E ; Scarlato, G. / Expression of the low-affinity NGF receptor during human muscle development, regeneration, and in tissue culture. In: Muscle and Nerve. 1994 ; Vol. 17, No. 3. pp. 276-284.
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