Abstract
Objective: To determine intrafollicular hormone levels and characterize the mRNA expression of the insulin-like growth factor (IGF) receptors, IGF binding proteins (IGFBP), and pregnancy-associated plasma protein-A (PAPP-A) in granulosa cells before and after an ovulatory hCG stimulus. Design: Experimental animal study. Setting: Academic medical center. Animal(s): Adult rhesus macaques. Intervention(s): Animals received exogenous FSH to promote the development of multiple preovulatory follicles. Follicles were aspirated before (0 hours) or 3, 6, 12, or 24 hours after an ovulatory hCG bolus. Main Outcome Measure(s): IGF1, IGF2, and insulin levels in follicular fluid were determined by radioimmunoassay. Messenger RNA (mRNA) levels in granulosa cells were determined by real-time reverse transcriptase-polymerase chain reaction. IGFBPs and PAPP-A in follicular fluid were determined by Western blot analysis and enzyme-linked immunosorbent assay. Result(s): IGF1, IGF2, and insulin in follicular fluid did not change during luteinization. IGF1R, IGFBP1, and IGFBP2 mRNAs were unchanged by hCG. IGF2R, IGFBP3, -5, and -6 and PAPP-A mRNA levels increased after hCG administration, while insulin receptor and IGFBP4 mRNA levels decreased after hCG administration. IGFBP3 and -6 and PAPP-A protein increased after hCG administration. Conclusion(s): Dynamic changes in the expression of the IGFBPs and PAPP-A suggest tight regulation of IGF action during ovulation and corpus luteum formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1421-1429 |
| Number of pages | 9 |
| Journal | Fertility and Sterility |
| Volume | 93 |
| Issue number | 5 |
| DOIs | |
| State | Published - Mar 15 2010 |
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Keywords
- granulosa cell
- IGF
- IGFBP
- Luteinization
- primate
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine
Cite this
Expression of the insulin-like growth factor and insulin systems in the luteinizing macaque ovarian follicle. / Brogan, Rebecca S.; Mix, Scott; Puttabyatappa, Muraly; VandeVoort, Catherine A.; Chaffin, Charles L.
In: Fertility and Sterility, Vol. 93, No. 5, 15.03.2010, p. 1421-1429.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of the insulin-like growth factor and insulin systems in the luteinizing macaque ovarian follicle
AU - Brogan, Rebecca S.
AU - Mix, Scott
AU - Puttabyatappa, Muraly
AU - VandeVoort, Catherine A.
AU - Chaffin, Charles L.
PY - 2010/3/15
Y1 - 2010/3/15
N2 - Objective: To determine intrafollicular hormone levels and characterize the mRNA expression of the insulin-like growth factor (IGF) receptors, IGF binding proteins (IGFBP), and pregnancy-associated plasma protein-A (PAPP-A) in granulosa cells before and after an ovulatory hCG stimulus. Design: Experimental animal study. Setting: Academic medical center. Animal(s): Adult rhesus macaques. Intervention(s): Animals received exogenous FSH to promote the development of multiple preovulatory follicles. Follicles were aspirated before (0 hours) or 3, 6, 12, or 24 hours after an ovulatory hCG bolus. Main Outcome Measure(s): IGF1, IGF2, and insulin levels in follicular fluid were determined by radioimmunoassay. Messenger RNA (mRNA) levels in granulosa cells were determined by real-time reverse transcriptase-polymerase chain reaction. IGFBPs and PAPP-A in follicular fluid were determined by Western blot analysis and enzyme-linked immunosorbent assay. Result(s): IGF1, IGF2, and insulin in follicular fluid did not change during luteinization. IGF1R, IGFBP1, and IGFBP2 mRNAs were unchanged by hCG. IGF2R, IGFBP3, -5, and -6 and PAPP-A mRNA levels increased after hCG administration, while insulin receptor and IGFBP4 mRNA levels decreased after hCG administration. IGFBP3 and -6 and PAPP-A protein increased after hCG administration. Conclusion(s): Dynamic changes in the expression of the IGFBPs and PAPP-A suggest tight regulation of IGF action during ovulation and corpus luteum formation.
AB - Objective: To determine intrafollicular hormone levels and characterize the mRNA expression of the insulin-like growth factor (IGF) receptors, IGF binding proteins (IGFBP), and pregnancy-associated plasma protein-A (PAPP-A) in granulosa cells before and after an ovulatory hCG stimulus. Design: Experimental animal study. Setting: Academic medical center. Animal(s): Adult rhesus macaques. Intervention(s): Animals received exogenous FSH to promote the development of multiple preovulatory follicles. Follicles were aspirated before (0 hours) or 3, 6, 12, or 24 hours after an ovulatory hCG bolus. Main Outcome Measure(s): IGF1, IGF2, and insulin levels in follicular fluid were determined by radioimmunoassay. Messenger RNA (mRNA) levels in granulosa cells were determined by real-time reverse transcriptase-polymerase chain reaction. IGFBPs and PAPP-A in follicular fluid were determined by Western blot analysis and enzyme-linked immunosorbent assay. Result(s): IGF1, IGF2, and insulin in follicular fluid did not change during luteinization. IGF1R, IGFBP1, and IGFBP2 mRNAs were unchanged by hCG. IGF2R, IGFBP3, -5, and -6 and PAPP-A mRNA levels increased after hCG administration, while insulin receptor and IGFBP4 mRNA levels decreased after hCG administration. IGFBP3 and -6 and PAPP-A protein increased after hCG administration. Conclusion(s): Dynamic changes in the expression of the IGFBPs and PAPP-A suggest tight regulation of IGF action during ovulation and corpus luteum formation.
KW - granulosa cell
KW - IGF
KW - IGFBP
KW - Luteinization
KW - primate
UR - http://www.scopus.com/inward/record.url?scp=77949263094&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77949263094&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2008.12.096
DO - 10.1016/j.fertnstert.2008.12.096
M3 - Article
C2 - 19243760
AN - SCOPUS:77949263094
VL - 93
SP - 1421
EP - 1429
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 5
ER -