Expression of the HML-1 epitope on human monocytes is independent of αE integrin mRNA

Lisa Miller, Congfen Li, Dallas M. Hyde

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Interferon gamma (IFNγ)-mediated activation of the myelomonocytic cell line HL-60 and peripheral blood monocytes will induce expression of an epitope for the monoclonal antibody which recognizes human αEβ7 integrin, HML-1. Here, we found that the anti-human αEβ7 monoclonal antibody Ber-ACT8 did not recognize IFNγ-stimulated, HML-1 positive HL-60 cells. Culture of blood monocytes with IFNγ also induced expression of HML-1 but not Ber-ACT8 epitopes. Moreover, migration of monocytes across a monolayer of human airway epithelial cells rapidly induced expression of HML-1, with no detectable Ber- ACT8 staining. Using two different sets of primers specific for the human αE integrin gene, we were unable to detect αE mRNA within HL-60 cells or isolated monocytes by reverse transcriptase polymerase chain reaction methods. We conclude that reactivity of the HML-1 antibody for HL-60 cells and monocytes does not correspond with the expression of the αE integrin subunit, and instead detects a marker for cellular activation.

Original languageEnglish (US)
Pages (from-to)195-205
Number of pages11
JournalInflammation
Volume24
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Immunology
  • Medicine(all)

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