Expression of the BabA adhesin during experimental infection with Helicobacter pylori

Cathy M. Styer, Lori M. Hansen, Cara L. Cooke, Amy M. Gundersen, Sung Sook Choi, Douglas E. Berg, Mohammed Benghezal, Barry J. Marshall, Richard M. Peek, Thomas Borén, Jay V Solnick

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

The Helicobacter pylori babA gene encodes an outer membrane protein that mediates binding to fucosylated ABH antigens of the ABO blood group. We recently demonstrated that BabA expression is lost during experimental infection of rhesus macaques with H. pylori J166. We sought to test the generality of this observation by comparison of different H. pylori strains and different animal hosts. Challenge of macaques with H. pylori J99 yielded output strains that lost BabA expression, either by selection and then expansion of a subpopulation of J99 that had a single-base-pair mutation that encoded a stop codon or by gene conversion of babA with a duplicate copy of babB, a paralog of unknown function. Challenge of mice with H. pylori J166, which unlike J99, has 5′ CT repeats in babA, resulted in loss of BabA expression due to phase variation. In the gerbil, Leb binding was lost by replacement of the babA gene that encoded Leb binding with a nonbinding allele that differed at six amino acid residues. Complementation experiments confirmed that change in these six amino acids of BabA was sufficient to eliminate binding to Leb and to gastric tissue. These results demonstrate that BabA expression in vivo is highly dynamic, and the findings implicate specific amino acid residues as critical for binding to fucosylated ABH antigens. We hypothesize that modification of BabA expression during H. pylori infection is a mechanism to adapt to changing conditions of inflammation and glycan expression at the epithelial surface.

Original languageEnglish (US)
Pages (from-to)1593-1600
Number of pages8
JournalInfection and Immunity
Volume78
Issue number4
DOIs
StatePublished - Apr 2010

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Helicobacter pylori
Infection
Amino Acids
Gene Conversion
Gerbillinae
Terminator Codon
Macaca
Helicobacter Infections
Blood Group Antigens
Macaca mulatta
Protein Binding
Base Pairing
Genes
Polysaccharides
Stomach
Membrane Proteins
Alleles
Inflammation
Antigens
Mutation

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Styer, C. M., Hansen, L. M., Cooke, C. L., Gundersen, A. M., Choi, S. S., Berg, D. E., ... Solnick, J. V. (2010). Expression of the BabA adhesin during experimental infection with Helicobacter pylori. Infection and Immunity, 78(4), 1593-1600. https://doi.org/10.1128/IAI.01297-09

Expression of the BabA adhesin during experimental infection with Helicobacter pylori. / Styer, Cathy M.; Hansen, Lori M.; Cooke, Cara L.; Gundersen, Amy M.; Choi, Sung Sook; Berg, Douglas E.; Benghezal, Mohammed; Marshall, Barry J.; Peek, Richard M.; Borén, Thomas; Solnick, Jay V.

In: Infection and Immunity, Vol. 78, No. 4, 04.2010, p. 1593-1600.

Research output: Contribution to journalArticle

Styer, CM, Hansen, LM, Cooke, CL, Gundersen, AM, Choi, SS, Berg, DE, Benghezal, M, Marshall, BJ, Peek, RM, Borén, T & Solnick, JV 2010, 'Expression of the BabA adhesin during experimental infection with Helicobacter pylori', Infection and Immunity, vol. 78, no. 4, pp. 1593-1600. https://doi.org/10.1128/IAI.01297-09
Styer CM, Hansen LM, Cooke CL, Gundersen AM, Choi SS, Berg DE et al. Expression of the BabA adhesin during experimental infection with Helicobacter pylori. Infection and Immunity. 2010 Apr;78(4):1593-1600. https://doi.org/10.1128/IAI.01297-09
Styer, Cathy M. ; Hansen, Lori M. ; Cooke, Cara L. ; Gundersen, Amy M. ; Choi, Sung Sook ; Berg, Douglas E. ; Benghezal, Mohammed ; Marshall, Barry J. ; Peek, Richard M. ; Borén, Thomas ; Solnick, Jay V. / Expression of the BabA adhesin during experimental infection with Helicobacter pylori. In: Infection and Immunity. 2010 ; Vol. 78, No. 4. pp. 1593-1600.
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