Expression of Th-2 cytokines interleukin-4 and -5 and of Th-1 cytokine interferon-γ in ovalbumin-exposed sensitized Brown-Norway rats

Angela Franciska Haczku, P. Macary, E. B. Haddad, T. J. Huang, D. M. Kemeny, R. Moqbel, K. F. Chung

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Abstract

We determined the expression of Th-2 type cytokines, interleukin-4 (IL-4) and IL-5, and of the Th-1 type cytokine, interferon-γ (IFN-γ), in the Brown-Norway rat. Rats were intraperitoneally sensitized with ovalbumin and 21 days later were either exposed to ovalbumin or saline aerosol. The value - log PC300 (PC300 = concentration of acetylcholine needed to increase baseline hung resistance by 300%) was 2.49 ± 0.15 in sensitized, exposed rats, was higher than in sensitized, saline-exposed or naive rats (1.54 ± 0.27 and 1.63 ± 0.06 respectively, P < 0.05). There was a significant increase in eosinophils in bronchoalveolar lavage fluid and in airway submucosal airway tissues in the sensitized exposed group. Reverse- transcriptase polymerase chain reaction was performed on total lung RNA using primers for IL-4, IL-5, IFN-γ and β-actin. IL-4 and IL-5 mRNA levels in control and sensitized saline-exposed rats were not detectable, but increased levels were found in sensitized and ovalbumin-exposed rats with levels of 0.25 ± 0.01 and 0.98 ± 0.02% of β-actin mRNA as assessed by densitometric measurements. Expression of IFN-γ mRNA was significantly reduced in sensitized and ovalbumin-exposed rats. As in asthmatic airways, there is an increased expression of Th-2 cytokines, IL-4 and IL-5, together with a reduction in the Th-1 cytokine, IFN-γ, thus supporting a role for Th-2 cytokines in allergic eosinophilic inflammation.

Original languageEnglish (US)
Pages (from-to)247-251
Number of pages5
JournalImmunology
Volume88
Issue number2
StatePublished - 1996
Externally publishedYes

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Interleukin-5
Ovalbumin
Interleukin-4
Interferons
Cytokines
Messenger RNA
Actins
Interferon Type I
Bronchoalveolar Lavage Fluid
Aerosols
Reverse Transcriptase Polymerase Chain Reaction
Eosinophils
Acetylcholine
Inflammation
Lung

ASJC Scopus subject areas

  • Immunology

Cite this

Haczku, A. F., Macary, P., Haddad, E. B., Huang, T. J., Kemeny, D. M., Moqbel, R., & Chung, K. F. (1996). Expression of Th-2 cytokines interleukin-4 and -5 and of Th-1 cytokine interferon-γ in ovalbumin-exposed sensitized Brown-Norway rats. Immunology, 88(2), 247-251.

Expression of Th-2 cytokines interleukin-4 and -5 and of Th-1 cytokine interferon-γ in ovalbumin-exposed sensitized Brown-Norway rats. / Haczku, Angela Franciska; Macary, P.; Haddad, E. B.; Huang, T. J.; Kemeny, D. M.; Moqbel, R.; Chung, K. F.

In: Immunology, Vol. 88, No. 2, 1996, p. 247-251.

Research output: Contribution to journalArticle

Haczku, AF, Macary, P, Haddad, EB, Huang, TJ, Kemeny, DM, Moqbel, R & Chung, KF 1996, 'Expression of Th-2 cytokines interleukin-4 and -5 and of Th-1 cytokine interferon-γ in ovalbumin-exposed sensitized Brown-Norway rats', Immunology, vol. 88, no. 2, pp. 247-251.
Haczku, Angela Franciska ; Macary, P. ; Haddad, E. B. ; Huang, T. J. ; Kemeny, D. M. ; Moqbel, R. ; Chung, K. F. / Expression of Th-2 cytokines interleukin-4 and -5 and of Th-1 cytokine interferon-γ in ovalbumin-exposed sensitized Brown-Norway rats. In: Immunology. 1996 ; Vol. 88, No. 2. pp. 247-251.
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abstract = "We determined the expression of Th-2 type cytokines, interleukin-4 (IL-4) and IL-5, and of the Th-1 type cytokine, interferon-γ (IFN-γ), in the Brown-Norway rat. Rats were intraperitoneally sensitized with ovalbumin and 21 days later were either exposed to ovalbumin or saline aerosol. The value - log PC300 (PC300 = concentration of acetylcholine needed to increase baseline hung resistance by 300{\%}) was 2.49 ± 0.15 in sensitized, exposed rats, was higher than in sensitized, saline-exposed or naive rats (1.54 ± 0.27 and 1.63 ± 0.06 respectively, P < 0.05). There was a significant increase in eosinophils in bronchoalveolar lavage fluid and in airway submucosal airway tissues in the sensitized exposed group. Reverse- transcriptase polymerase chain reaction was performed on total lung RNA using primers for IL-4, IL-5, IFN-γ and β-actin. IL-4 and IL-5 mRNA levels in control and sensitized saline-exposed rats were not detectable, but increased levels were found in sensitized and ovalbumin-exposed rats with levels of 0.25 ± 0.01 and 0.98 ± 0.02{\%} of β-actin mRNA as assessed by densitometric measurements. Expression of IFN-γ mRNA was significantly reduced in sensitized and ovalbumin-exposed rats. As in asthmatic airways, there is an increased expression of Th-2 cytokines, IL-4 and IL-5, together with a reduction in the Th-1 cytokine, IFN-γ, thus supporting a role for Th-2 cytokines in allergic eosinophilic inflammation.",
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AU - Haddad, E. B.

AU - Huang, T. J.

AU - Kemeny, D. M.

AU - Moqbel, R.

AU - Chung, K. F.

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N2 - We determined the expression of Th-2 type cytokines, interleukin-4 (IL-4) and IL-5, and of the Th-1 type cytokine, interferon-γ (IFN-γ), in the Brown-Norway rat. Rats were intraperitoneally sensitized with ovalbumin and 21 days later were either exposed to ovalbumin or saline aerosol. The value - log PC300 (PC300 = concentration of acetylcholine needed to increase baseline hung resistance by 300%) was 2.49 ± 0.15 in sensitized, exposed rats, was higher than in sensitized, saline-exposed or naive rats (1.54 ± 0.27 and 1.63 ± 0.06 respectively, P < 0.05). There was a significant increase in eosinophils in bronchoalveolar lavage fluid and in airway submucosal airway tissues in the sensitized exposed group. Reverse- transcriptase polymerase chain reaction was performed on total lung RNA using primers for IL-4, IL-5, IFN-γ and β-actin. IL-4 and IL-5 mRNA levels in control and sensitized saline-exposed rats were not detectable, but increased levels were found in sensitized and ovalbumin-exposed rats with levels of 0.25 ± 0.01 and 0.98 ± 0.02% of β-actin mRNA as assessed by densitometric measurements. Expression of IFN-γ mRNA was significantly reduced in sensitized and ovalbumin-exposed rats. As in asthmatic airways, there is an increased expression of Th-2 cytokines, IL-4 and IL-5, together with a reduction in the Th-1 cytokine, IFN-γ, thus supporting a role for Th-2 cytokines in allergic eosinophilic inflammation.

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