Abstract
SPARC (secreted protein, acidic and rich in cysteine)-also known as osteonectin, BM-40, and 43K glycoprotein-is secreted by endothelial cells and fibroblasts in response to culture shock. SPARC has been found in association with tissues undergoing cell proliferation, migration, and extracellular matrix remodeling. We demonstrate that normal livers from humans, rats, and mice express substantial levels of SPARC messenger RNA (mRNA). Moreover, when compared with control specimens, significantly increased levels of SPARC mRNA were found in fibrotic livers from two animal models of liver disease: murine schistosomiasis and carbon tetrachloride-induced fibrosis in rats. Fibrotic human livers also had markedly increased levels of SPARC mRNA in comparison with normal livers. We also detected an increased production of SPARC protein in the liver of animals treated with carbon tetrachloride. By immunocytochemical analysis, SPARC protein was apparent in freshly isolated Ito cells. Hybridization studies showed Ito cells to be the main source of SPARC mRNA. Extracts from a Kupffer-endothelial cell fraction exhibited traces of SPARC transcript, but expression of SPARC mRNA was absent in extracts from freshly isolated hepatocytes. These studies demonstrate the increased expression of SPARC-a protein that modulates cell shape and disrupts cell-matrix interactions-during the initial stages of hepatic fibrosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 847-854 |
| Number of pages | 8 |
| Journal | Hepatology |
| Volume | 21 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1995 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hepatology
Cite this
Expression of SPARC in normal and fibrotic livers. / Frizell, Edward; Liu, Shu Ling; Abraham, Ann; Ozaki, Iwata; Eghbali, Mahboubeh; Helene Sage, E.; Zern, Mark A.
In: Hepatology, Vol. 21, No. 3, 1995, p. 847-854.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of SPARC in normal and fibrotic livers
AU - Frizell, Edward
AU - Liu, Shu Ling
AU - Abraham, Ann
AU - Ozaki, Iwata
AU - Eghbali, Mahboubeh
AU - Helene Sage, E.
AU - Zern, Mark A
PY - 1995
Y1 - 1995
N2 - SPARC (secreted protein, acidic and rich in cysteine)-also known as osteonectin, BM-40, and 43K glycoprotein-is secreted by endothelial cells and fibroblasts in response to culture shock. SPARC has been found in association with tissues undergoing cell proliferation, migration, and extracellular matrix remodeling. We demonstrate that normal livers from humans, rats, and mice express substantial levels of SPARC messenger RNA (mRNA). Moreover, when compared with control specimens, significantly increased levels of SPARC mRNA were found in fibrotic livers from two animal models of liver disease: murine schistosomiasis and carbon tetrachloride-induced fibrosis in rats. Fibrotic human livers also had markedly increased levels of SPARC mRNA in comparison with normal livers. We also detected an increased production of SPARC protein in the liver of animals treated with carbon tetrachloride. By immunocytochemical analysis, SPARC protein was apparent in freshly isolated Ito cells. Hybridization studies showed Ito cells to be the main source of SPARC mRNA. Extracts from a Kupffer-endothelial cell fraction exhibited traces of SPARC transcript, but expression of SPARC mRNA was absent in extracts from freshly isolated hepatocytes. These studies demonstrate the increased expression of SPARC-a protein that modulates cell shape and disrupts cell-matrix interactions-during the initial stages of hepatic fibrosis.
AB - SPARC (secreted protein, acidic and rich in cysteine)-also known as osteonectin, BM-40, and 43K glycoprotein-is secreted by endothelial cells and fibroblasts in response to culture shock. SPARC has been found in association with tissues undergoing cell proliferation, migration, and extracellular matrix remodeling. We demonstrate that normal livers from humans, rats, and mice express substantial levels of SPARC messenger RNA (mRNA). Moreover, when compared with control specimens, significantly increased levels of SPARC mRNA were found in fibrotic livers from two animal models of liver disease: murine schistosomiasis and carbon tetrachloride-induced fibrosis in rats. Fibrotic human livers also had markedly increased levels of SPARC mRNA in comparison with normal livers. We also detected an increased production of SPARC protein in the liver of animals treated with carbon tetrachloride. By immunocytochemical analysis, SPARC protein was apparent in freshly isolated Ito cells. Hybridization studies showed Ito cells to be the main source of SPARC mRNA. Extracts from a Kupffer-endothelial cell fraction exhibited traces of SPARC transcript, but expression of SPARC mRNA was absent in extracts from freshly isolated hepatocytes. These studies demonstrate the increased expression of SPARC-a protein that modulates cell shape and disrupts cell-matrix interactions-during the initial stages of hepatic fibrosis.
UR - http://www.scopus.com/inward/record.url?scp=0028967282&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028967282&partnerID=8YFLogxK
U2 - 10.1016/0270-9139(95)90540-5
DO - 10.1016/0270-9139(95)90540-5
M3 - Article
C2 - 7875683
AN - SCOPUS:0028967282
VL - 21
SP - 847
EP - 854
JO - Hepatology
JF - Hepatology
SN - 0270-9139
IS - 3
ER -