Expression of SPARC in normal and fibrotic livers

Edward Frizell, Shu Ling Liu, Ann Abraham, Iwata Ozaki, Mahboubeh Eghbali, E. Helene Sage, Mark A Zern

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

SPARC (secreted protein, acidic and rich in cysteine)-also known as osteonectin, BM-40, and 43K glycoprotein-is secreted by endothelial cells and fibroblasts in response to culture shock. SPARC has been found in association with tissues undergoing cell proliferation, migration, and extracellular matrix remodeling. We demonstrate that normal livers from humans, rats, and mice express substantial levels of SPARC messenger RNA (mRNA). Moreover, when compared with control specimens, significantly increased levels of SPARC mRNA were found in fibrotic livers from two animal models of liver disease: murine schistosomiasis and carbon tetrachloride-induced fibrosis in rats. Fibrotic human livers also had markedly increased levels of SPARC mRNA in comparison with normal livers. We also detected an increased production of SPARC protein in the liver of animals treated with carbon tetrachloride. By immunocytochemical analysis, SPARC protein was apparent in freshly isolated Ito cells. Hybridization studies showed Ito cells to be the main source of SPARC mRNA. Extracts from a Kupffer-endothelial cell fraction exhibited traces of SPARC transcript, but expression of SPARC mRNA was absent in extracts from freshly isolated hepatocytes. These studies demonstrate the increased expression of SPARC-a protein that modulates cell shape and disrupts cell-matrix interactions-during the initial stages of hepatic fibrosis.

Original languageEnglish (US)
Pages (from-to)847-854
Number of pages8
JournalHepatology
Volume21
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Cysteine
Liver
Proteins
Messenger RNA
Osteonectin
Hepatic Stellate Cells
Carbon Tetrachloride
Fibrosis
Endothelial Cells
Kupffer Cells
Cell Shape
Schistosomiasis
Cell Communication
Cell Movement
Extracellular Matrix
Liver Diseases
Hepatocytes
Shock
Glycoproteins
Animal Models

ASJC Scopus subject areas

  • Hepatology

Cite this

Frizell, E., Liu, S. L., Abraham, A., Ozaki, I., Eghbali, M., Helene Sage, E., & Zern, M. A. (1995). Expression of SPARC in normal and fibrotic livers. Hepatology, 21(3), 847-854. https://doi.org/10.1016/0270-9139(95)90540-5

Expression of SPARC in normal and fibrotic livers. / Frizell, Edward; Liu, Shu Ling; Abraham, Ann; Ozaki, Iwata; Eghbali, Mahboubeh; Helene Sage, E.; Zern, Mark A.

In: Hepatology, Vol. 21, No. 3, 1995, p. 847-854.

Research output: Contribution to journalArticle

Frizell, E, Liu, SL, Abraham, A, Ozaki, I, Eghbali, M, Helene Sage, E & Zern, MA 1995, 'Expression of SPARC in normal and fibrotic livers', Hepatology, vol. 21, no. 3, pp. 847-854. https://doi.org/10.1016/0270-9139(95)90540-5
Frizell E, Liu SL, Abraham A, Ozaki I, Eghbali M, Helene Sage E et al. Expression of SPARC in normal and fibrotic livers. Hepatology. 1995;21(3):847-854. https://doi.org/10.1016/0270-9139(95)90540-5
Frizell, Edward ; Liu, Shu Ling ; Abraham, Ann ; Ozaki, Iwata ; Eghbali, Mahboubeh ; Helene Sage, E. ; Zern, Mark A. / Expression of SPARC in normal and fibrotic livers. In: Hepatology. 1995 ; Vol. 21, No. 3. pp. 847-854.
@article{fde30f23ab9c404aa53f4c58f55e112b,
title = "Expression of SPARC in normal and fibrotic livers",
abstract = "SPARC (secreted protein, acidic and rich in cysteine)-also known as osteonectin, BM-40, and 43K glycoprotein-is secreted by endothelial cells and fibroblasts in response to culture shock. SPARC has been found in association with tissues undergoing cell proliferation, migration, and extracellular matrix remodeling. We demonstrate that normal livers from humans, rats, and mice express substantial levels of SPARC messenger RNA (mRNA). Moreover, when compared with control specimens, significantly increased levels of SPARC mRNA were found in fibrotic livers from two animal models of liver disease: murine schistosomiasis and carbon tetrachloride-induced fibrosis in rats. Fibrotic human livers also had markedly increased levels of SPARC mRNA in comparison with normal livers. We also detected an increased production of SPARC protein in the liver of animals treated with carbon tetrachloride. By immunocytochemical analysis, SPARC protein was apparent in freshly isolated Ito cells. Hybridization studies showed Ito cells to be the main source of SPARC mRNA. Extracts from a Kupffer-endothelial cell fraction exhibited traces of SPARC transcript, but expression of SPARC mRNA was absent in extracts from freshly isolated hepatocytes. These studies demonstrate the increased expression of SPARC-a protein that modulates cell shape and disrupts cell-matrix interactions-during the initial stages of hepatic fibrosis.",
author = "Edward Frizell and Liu, {Shu Ling} and Ann Abraham and Iwata Ozaki and Mahboubeh Eghbali and {Helene Sage}, E. and Zern, {Mark A}",
year = "1995",
doi = "10.1016/0270-9139(95)90540-5",
language = "English (US)",
volume = "21",
pages = "847--854",
journal = "Hepatology",
issn = "0270-9139",
publisher = "John Wiley and Sons Ltd",
number = "3",

}

TY - JOUR

T1 - Expression of SPARC in normal and fibrotic livers

AU - Frizell, Edward

AU - Liu, Shu Ling

AU - Abraham, Ann

AU - Ozaki, Iwata

AU - Eghbali, Mahboubeh

AU - Helene Sage, E.

AU - Zern, Mark A

PY - 1995

Y1 - 1995

N2 - SPARC (secreted protein, acidic and rich in cysteine)-also known as osteonectin, BM-40, and 43K glycoprotein-is secreted by endothelial cells and fibroblasts in response to culture shock. SPARC has been found in association with tissues undergoing cell proliferation, migration, and extracellular matrix remodeling. We demonstrate that normal livers from humans, rats, and mice express substantial levels of SPARC messenger RNA (mRNA). Moreover, when compared with control specimens, significantly increased levels of SPARC mRNA were found in fibrotic livers from two animal models of liver disease: murine schistosomiasis and carbon tetrachloride-induced fibrosis in rats. Fibrotic human livers also had markedly increased levels of SPARC mRNA in comparison with normal livers. We also detected an increased production of SPARC protein in the liver of animals treated with carbon tetrachloride. By immunocytochemical analysis, SPARC protein was apparent in freshly isolated Ito cells. Hybridization studies showed Ito cells to be the main source of SPARC mRNA. Extracts from a Kupffer-endothelial cell fraction exhibited traces of SPARC transcript, but expression of SPARC mRNA was absent in extracts from freshly isolated hepatocytes. These studies demonstrate the increased expression of SPARC-a protein that modulates cell shape and disrupts cell-matrix interactions-during the initial stages of hepatic fibrosis.

AB - SPARC (secreted protein, acidic and rich in cysteine)-also known as osteonectin, BM-40, and 43K glycoprotein-is secreted by endothelial cells and fibroblasts in response to culture shock. SPARC has been found in association with tissues undergoing cell proliferation, migration, and extracellular matrix remodeling. We demonstrate that normal livers from humans, rats, and mice express substantial levels of SPARC messenger RNA (mRNA). Moreover, when compared with control specimens, significantly increased levels of SPARC mRNA were found in fibrotic livers from two animal models of liver disease: murine schistosomiasis and carbon tetrachloride-induced fibrosis in rats. Fibrotic human livers also had markedly increased levels of SPARC mRNA in comparison with normal livers. We also detected an increased production of SPARC protein in the liver of animals treated with carbon tetrachloride. By immunocytochemical analysis, SPARC protein was apparent in freshly isolated Ito cells. Hybridization studies showed Ito cells to be the main source of SPARC mRNA. Extracts from a Kupffer-endothelial cell fraction exhibited traces of SPARC transcript, but expression of SPARC mRNA was absent in extracts from freshly isolated hepatocytes. These studies demonstrate the increased expression of SPARC-a protein that modulates cell shape and disrupts cell-matrix interactions-during the initial stages of hepatic fibrosis.

UR - http://www.scopus.com/inward/record.url?scp=0028967282&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028967282&partnerID=8YFLogxK

U2 - 10.1016/0270-9139(95)90540-5

DO - 10.1016/0270-9139(95)90540-5

M3 - Article

C2 - 7875683

AN - SCOPUS:0028967282

VL - 21

SP - 847

EP - 854

JO - Hepatology

JF - Hepatology

SN - 0270-9139

IS - 3

ER -