Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli

T. W. North, G. L. Hansen, Y. Zhu, J. A. Griffin, C. K. Shih

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11 Scopus citations

Abstract

Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'- triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'- dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.

Original languageEnglish (US)
Pages (from-to)388-391
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume38
Issue number2
StatePublished - 1994
Externally publishedYes

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ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

North, T. W., Hansen, G. L., Zhu, Y., Griffin, J. A., & Shih, C. K. (1994). Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli. Antimicrobial Agents and Chemotherapy, 38(2), 388-391.