Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'- triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'- dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.
|Original language||English (US)|
|Number of pages||4|
|Journal||Antimicrobial Agents and Chemotherapy|
|State||Published - 1994|
ASJC Scopus subject areas
- Pharmacology (medical)