Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli

T. W. North, G. L. Hansen, Y. Zhu, J. A. Griffin, C. K. Shih

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'- triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'- dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.

Original languageEnglish (US)
Pages (from-to)388-391
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume38
Issue number2
StatePublished - 1994
Externally publishedYes

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Feline Immunodeficiency Virus
RNA-Directed DNA Polymerase
Escherichia coli
Dideoxynucleosides
Virion
Pharmacology
Enzymes
Proteins

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

North, T. W., Hansen, G. L., Zhu, Y., Griffin, J. A., & Shih, C. K. (1994). Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli. Antimicrobial Agents and Chemotherapy, 38(2), 388-391.

Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli. / North, T. W.; Hansen, G. L.; Zhu, Y.; Griffin, J. A.; Shih, C. K.

In: Antimicrobial Agents and Chemotherapy, Vol. 38, No. 2, 1994, p. 388-391.

Research output: Contribution to journalArticle

North, TW, Hansen, GL, Zhu, Y, Griffin, JA & Shih, CK 1994, 'Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli', Antimicrobial Agents and Chemotherapy, vol. 38, no. 2, pp. 388-391.
North, T. W. ; Hansen, G. L. ; Zhu, Y. ; Griffin, J. A. ; Shih, C. K. / Expression of reverse transcriptase from feline immunodeficiency virus in Escherichia coli. In: Antimicrobial Agents and Chemotherapy. 1994 ; Vol. 38, No. 2. pp. 388-391.
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