Expression of retinoic acid receptor genes in developing rat livers and hepatoma cells

Yu-Jui Yvonne Wan, L. Wang, T. C J Wu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The expression of retinoic acid receptor α, β, and Γ mRNA was examined in developing rat livers and rat hepatoma-derived cell lines H-4-II-E, McA-RH 7777, and 8994 that represent different hepatocyte phenotypes. Northern blot hybridization demonstrated that all three receptor mRNAs were expressed in the fetal livers of different gestational ages, and the levels of expression increased significantly 3 to 4 weeks after birth. In the hepatoma cell lines, the expression pattern of retinoic acid receptor α and Γ mRNA did not correlate with the phenotype. In contrast, retinoic acid receptor β mRNA was only detected in the adult phenotypic H-4-II-E cells but not in McA-RH 7777 and 8994 cells, which represent embryonic and fetal hepatocyte phenotypes, respectively. The levels of retinoic acid receptor β mRNA in hepatoma cell lines were lower than adult rat liver. These data suggest that the increased expression of retinoic acid receptor β gene is associated with differentiation or maturation of rat hepatocytes. The effect of retinoic acid on retinoic acid receptor gene expression was also studied in hepatoma cells. Retinoic acid did not regulate retinoic acid receptor gene expression in McA- RH 7777 and 8994 cells, and the retinoic acid receptor β gene remained inactivated in these cells. However, Southern blot hybridization indicated that the gross structure of retinoic acid receptor β gene was not altered during malignant transformation. In H-4-II-E cells, retinoic acid increased the expression of retinoic acid receptor β and Γ gene. Because of the similarity between H-4-II-E cells and normal adult hepatocytes, this type of autoregulation may be a mechanism by which retinoic acid regulates its own effect in vivo.

Original languageEnglish (US)
Pages (from-to)646-651
Number of pages6
JournalLaboratory Investigation
Volume66
Issue number5
StatePublished - 1992

Fingerprint

Retinoic Acid Receptors
Hepatocellular Carcinoma
Liver
Genes
Tretinoin
Hepatocytes
Messenger RNA
Phenotype
Cell Line
Gene Expression
Southern Blotting
Northern Blotting
Gestational Age
Homeostasis
Parturition

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of retinoic acid receptor genes in developing rat livers and hepatoma cells. / Wan, Yu-Jui Yvonne; Wang, L.; Wu, T. C J.

In: Laboratory Investigation, Vol. 66, No. 5, 1992, p. 646-651.

Research output: Contribution to journalArticle

@article{85dc06a86e7147b7929095254dace714,
title = "Expression of retinoic acid receptor genes in developing rat livers and hepatoma cells",
abstract = "The expression of retinoic acid receptor α, β, and Γ mRNA was examined in developing rat livers and rat hepatoma-derived cell lines H-4-II-E, McA-RH 7777, and 8994 that represent different hepatocyte phenotypes. Northern blot hybridization demonstrated that all three receptor mRNAs were expressed in the fetal livers of different gestational ages, and the levels of expression increased significantly 3 to 4 weeks after birth. In the hepatoma cell lines, the expression pattern of retinoic acid receptor α and Γ mRNA did not correlate with the phenotype. In contrast, retinoic acid receptor β mRNA was only detected in the adult phenotypic H-4-II-E cells but not in McA-RH 7777 and 8994 cells, which represent embryonic and fetal hepatocyte phenotypes, respectively. The levels of retinoic acid receptor β mRNA in hepatoma cell lines were lower than adult rat liver. These data suggest that the increased expression of retinoic acid receptor β gene is associated with differentiation or maturation of rat hepatocytes. The effect of retinoic acid on retinoic acid receptor gene expression was also studied in hepatoma cells. Retinoic acid did not regulate retinoic acid receptor gene expression in McA- RH 7777 and 8994 cells, and the retinoic acid receptor β gene remained inactivated in these cells. However, Southern blot hybridization indicated that the gross structure of retinoic acid receptor β gene was not altered during malignant transformation. In H-4-II-E cells, retinoic acid increased the expression of retinoic acid receptor β and Γ gene. Because of the similarity between H-4-II-E cells and normal adult hepatocytes, this type of autoregulation may be a mechanism by which retinoic acid regulates its own effect in vivo.",
author = "Wan, {Yu-Jui Yvonne} and L. Wang and Wu, {T. C J}",
year = "1992",
language = "English (US)",
volume = "66",
pages = "646--651",
journal = "Laboratory Investigation",
issn = "0023-6837",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Expression of retinoic acid receptor genes in developing rat livers and hepatoma cells

AU - Wan, Yu-Jui Yvonne

AU - Wang, L.

AU - Wu, T. C J

PY - 1992

Y1 - 1992

N2 - The expression of retinoic acid receptor α, β, and Γ mRNA was examined in developing rat livers and rat hepatoma-derived cell lines H-4-II-E, McA-RH 7777, and 8994 that represent different hepatocyte phenotypes. Northern blot hybridization demonstrated that all three receptor mRNAs were expressed in the fetal livers of different gestational ages, and the levels of expression increased significantly 3 to 4 weeks after birth. In the hepatoma cell lines, the expression pattern of retinoic acid receptor α and Γ mRNA did not correlate with the phenotype. In contrast, retinoic acid receptor β mRNA was only detected in the adult phenotypic H-4-II-E cells but not in McA-RH 7777 and 8994 cells, which represent embryonic and fetal hepatocyte phenotypes, respectively. The levels of retinoic acid receptor β mRNA in hepatoma cell lines were lower than adult rat liver. These data suggest that the increased expression of retinoic acid receptor β gene is associated with differentiation or maturation of rat hepatocytes. The effect of retinoic acid on retinoic acid receptor gene expression was also studied in hepatoma cells. Retinoic acid did not regulate retinoic acid receptor gene expression in McA- RH 7777 and 8994 cells, and the retinoic acid receptor β gene remained inactivated in these cells. However, Southern blot hybridization indicated that the gross structure of retinoic acid receptor β gene was not altered during malignant transformation. In H-4-II-E cells, retinoic acid increased the expression of retinoic acid receptor β and Γ gene. Because of the similarity between H-4-II-E cells and normal adult hepatocytes, this type of autoregulation may be a mechanism by which retinoic acid regulates its own effect in vivo.

AB - The expression of retinoic acid receptor α, β, and Γ mRNA was examined in developing rat livers and rat hepatoma-derived cell lines H-4-II-E, McA-RH 7777, and 8994 that represent different hepatocyte phenotypes. Northern blot hybridization demonstrated that all three receptor mRNAs were expressed in the fetal livers of different gestational ages, and the levels of expression increased significantly 3 to 4 weeks after birth. In the hepatoma cell lines, the expression pattern of retinoic acid receptor α and Γ mRNA did not correlate with the phenotype. In contrast, retinoic acid receptor β mRNA was only detected in the adult phenotypic H-4-II-E cells but not in McA-RH 7777 and 8994 cells, which represent embryonic and fetal hepatocyte phenotypes, respectively. The levels of retinoic acid receptor β mRNA in hepatoma cell lines were lower than adult rat liver. These data suggest that the increased expression of retinoic acid receptor β gene is associated with differentiation or maturation of rat hepatocytes. The effect of retinoic acid on retinoic acid receptor gene expression was also studied in hepatoma cells. Retinoic acid did not regulate retinoic acid receptor gene expression in McA- RH 7777 and 8994 cells, and the retinoic acid receptor β gene remained inactivated in these cells. However, Southern blot hybridization indicated that the gross structure of retinoic acid receptor β gene was not altered during malignant transformation. In H-4-II-E cells, retinoic acid increased the expression of retinoic acid receptor β and Γ gene. Because of the similarity between H-4-II-E cells and normal adult hepatocytes, this type of autoregulation may be a mechanism by which retinoic acid regulates its own effect in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0026631445&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026631445&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 646

EP - 651

JO - Laboratory Investigation

JF - Laboratory Investigation

SN - 0023-6837

IS - 5

ER -