Abstract
Lym-I single-chain Fv (sFv) can be used for targeted radiodiagnosis and therapy of B-lymphocytic malignancies. Lym-I sFv was constructed and expressed as a glutathione S-transferase fusion protein, using a (G4S)3 linker connecting the C-terminus of the V(H) domain and the N-terminus of the V(L) domain of Lym-I. Six histidine residues and an E Tag epitope were introduced at the C-terminus of the sFv. Lym-I sFv was purified with glutathione-Sepharose 4B affinity chromatography followed by digestion with thrombin. Lym-I sFv of 28 kDa was confirmed by Western blotting with anti-(E Tag) monoclonal antibody. An antigen binding assay of Lym-I and a CD study indicated that it is functionally active.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 163-167 |
| Number of pages | 5 |
| Journal | Biotechnology and Applied Biochemistry |
| Volume | 28 |
| Issue number | 2 |
| State | Published - 1998 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
Cite this
Expression of recombinant Lym-I single-chain Fv in Escherichia coli. / Song, Kyung Bin; Won, Misun; Meares, Claude F.
In: Biotechnology and Applied Biochemistry, Vol. 28, No. 2, 1998, p. 163-167.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of recombinant Lym-I single-chain Fv in Escherichia coli
AU - Song, Kyung Bin
AU - Won, Misun
AU - Meares, Claude F.
PY - 1998
Y1 - 1998
N2 - Lym-I single-chain Fv (sFv) can be used for targeted radiodiagnosis and therapy of B-lymphocytic malignancies. Lym-I sFv was constructed and expressed as a glutathione S-transferase fusion protein, using a (G4S)3 linker connecting the C-terminus of the V(H) domain and the N-terminus of the V(L) domain of Lym-I. Six histidine residues and an E Tag epitope were introduced at the C-terminus of the sFv. Lym-I sFv was purified with glutathione-Sepharose 4B affinity chromatography followed by digestion with thrombin. Lym-I sFv of 28 kDa was confirmed by Western blotting with anti-(E Tag) monoclonal antibody. An antigen binding assay of Lym-I and a CD study indicated that it is functionally active.
AB - Lym-I single-chain Fv (sFv) can be used for targeted radiodiagnosis and therapy of B-lymphocytic malignancies. Lym-I sFv was constructed and expressed as a glutathione S-transferase fusion protein, using a (G4S)3 linker connecting the C-terminus of the V(H) domain and the N-terminus of the V(L) domain of Lym-I. Six histidine residues and an E Tag epitope were introduced at the C-terminus of the sFv. Lym-I sFv was purified with glutathione-Sepharose 4B affinity chromatography followed by digestion with thrombin. Lym-I sFv of 28 kDa was confirmed by Western blotting with anti-(E Tag) monoclonal antibody. An antigen binding assay of Lym-I and a CD study indicated that it is functionally active.
UR - http://www.scopus.com/inward/record.url?scp=0031677793&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031677793&partnerID=8YFLogxK
M3 - Article
C2 - 9756467
AN - SCOPUS:0031677793
VL - 28
SP - 163
EP - 167
JO - Biotechnology and Applied Biochemistry
JF - Biotechnology and Applied Biochemistry
SN - 0885-4513
IS - 2
ER -