Lym-I single-chain Fv (sFv) can be used for targeted radiodiagnosis and therapy of B-lymphocytic malignancies. Lym-I sFv was constructed and expressed as a glutathione S-transferase fusion protein, using a (G4S)3 linker connecting the C-terminus of the V(H) domain and the N-terminus of the V(L) domain of Lym-I. Six histidine residues and an E Tag epitope were introduced at the C-terminus of the sFv. Lym-I sFv was purified with glutathione-Sepharose 4B affinity chromatography followed by digestion with thrombin. Lym-I sFv of 28 kDa was confirmed by Western blotting with anti-(E Tag) monoclonal antibody. An antigen binding assay of Lym-I and a CD study indicated that it is functionally active.
|Original language||English (US)|
|Number of pages||5|
|Journal||Biotechnology and Applied Biochemistry|
|State||Published - 1998|
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)