Abstract
Steady-state levels of rat central nervous system (CNS) platelet-derived growth factor (PDGF) A- and B-chain mRNAs were measured by a polymerase chain reaction method employing a synthetic gene internal standard, and the rates of transcription of PDGF A- and B-chain genes in CNS were estimated by a nuclear runoff assay. The abundance of PDGF B-chain mRNA was an order of magnitude below that of PDGF A-chain mRNA, while the rate of PDGF B-chain transcription was only slightly below that for the PDGF A-chain gene, indicating that the half-life of PDGF B-chain in CNS is shorter than that of PDGF A-chain mRNA. No developmental alterations in expression of the PDGF A- and B-chain genes were detected. By contrast, Northern blots showed that steady-state levels of mRNAs encoding the two PDGF receptor proteins, α and β, were markedly higher in embryonic day 15 and postnatal day 6 rat brains than in later life. These results suggest that the actions of PDGF on the brain in vivo are regulated not at the level of PDGF A and B-chain gene expression, but rather by changes in the level of expression of PDGF α- and β-receptor genes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 670-677 |
| Number of pages | 8 |
| Journal | Journal of Neuroscience Research |
| Volume | 31 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1992 |
| Externally published | Yes |
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Keywords
- brain
- PDGF
- PDGF receptors
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Expression of platelet-derived growth factor (PDGF) and PDGF receptor genes in the developing rat brain. / Reddy, U. R.; Pleasure, David E.
In: Journal of Neuroscience Research, Vol. 31, No. 4, 1992, p. 670-677.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of platelet-derived growth factor (PDGF) and PDGF receptor genes in the developing rat brain
AU - Reddy, U. R.
AU - Pleasure, David E
PY - 1992
Y1 - 1992
N2 - Steady-state levels of rat central nervous system (CNS) platelet-derived growth factor (PDGF) A- and B-chain mRNAs were measured by a polymerase chain reaction method employing a synthetic gene internal standard, and the rates of transcription of PDGF A- and B-chain genes in CNS were estimated by a nuclear runoff assay. The abundance of PDGF B-chain mRNA was an order of magnitude below that of PDGF A-chain mRNA, while the rate of PDGF B-chain transcription was only slightly below that for the PDGF A-chain gene, indicating that the half-life of PDGF B-chain in CNS is shorter than that of PDGF A-chain mRNA. No developmental alterations in expression of the PDGF A- and B-chain genes were detected. By contrast, Northern blots showed that steady-state levels of mRNAs encoding the two PDGF receptor proteins, α and β, were markedly higher in embryonic day 15 and postnatal day 6 rat brains than in later life. These results suggest that the actions of PDGF on the brain in vivo are regulated not at the level of PDGF A and B-chain gene expression, but rather by changes in the level of expression of PDGF α- and β-receptor genes.
AB - Steady-state levels of rat central nervous system (CNS) platelet-derived growth factor (PDGF) A- and B-chain mRNAs were measured by a polymerase chain reaction method employing a synthetic gene internal standard, and the rates of transcription of PDGF A- and B-chain genes in CNS were estimated by a nuclear runoff assay. The abundance of PDGF B-chain mRNA was an order of magnitude below that of PDGF A-chain mRNA, while the rate of PDGF B-chain transcription was only slightly below that for the PDGF A-chain gene, indicating that the half-life of PDGF B-chain in CNS is shorter than that of PDGF A-chain mRNA. No developmental alterations in expression of the PDGF A- and B-chain genes were detected. By contrast, Northern blots showed that steady-state levels of mRNAs encoding the two PDGF receptor proteins, α and β, were markedly higher in embryonic day 15 and postnatal day 6 rat brains than in later life. These results suggest that the actions of PDGF on the brain in vivo are regulated not at the level of PDGF A and B-chain gene expression, but rather by changes in the level of expression of PDGF α- and β-receptor genes.
KW - brain
KW - PDGF
KW - PDGF receptors
UR - http://www.scopus.com/inward/record.url?scp=0026770345&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026770345&partnerID=8YFLogxK
U2 - 10.1002/jnr.490310411
DO - 10.1002/jnr.490310411
M3 - Article
C2 - 1315873
AN - SCOPUS:0026770345
VL - 31
SP - 670
EP - 677
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
SN - 0360-4012
IS - 4
ER -