Expression of P0 protein mRNA along rat sciatic nerve during development

Pierluigi Baron, Michael Shy, John Kamholz, Guglielmo Scarlato, David E Pleasure

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


The temporal and spatial expression of P0 protein mRNA in the spinal nerve roots and in distal (popliteal trifurcation) segments of the developing rat sciatic nerve was examined using in situ hybridization. P0 mRNA was undetectable in the PNS portions at day 16 post-fertilization (E16) but present in the roots as well as in the sciatic nerve by E18. While Schwann cells were labelled by in situ hybridization at E18, it was not until birth (P1) that cells revealed the typical clusters of silver grains in a perinuclear distribution. Hybridization signal rose between P5 and P10, than decreased at P40, with no significant difference of P0 mRNA levels between the different PNS portions at each age studied. These findings demonstrate the lack of proximo-distal gradient in abundance of P0 mRNA during rat PNS development and add further insight in understanding the dynamics of axonal regulation of myelination in the PNS.

Original languageEnglish (US)
Pages (from-to)285-288
Number of pages4
JournalDevelopmental Brain Research
Issue number2
StatePublished - Dec 16 1994
Externally publishedYes


  • Development
  • In situ hybridization
  • P0 protein mRNA
  • Sciatic nerve
  • Spinal root

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience


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