Abstract
The temporal and spatial expression of P0 protein mRNA in the spinal nerve roots and in distal (popliteal trifurcation) segments of the developing rat sciatic nerve was examined using in situ hybridization. P0 mRNA was undetectable in the PNS portions at day 16 post-fertilization (E16) but present in the roots as well as in the sciatic nerve by E18. While Schwann cells were labelled by in situ hybridization at E18, it was not until birth (P1) that cells revealed the typical clusters of silver grains in a perinuclear distribution. Hybridization signal rose between P5 and P10, than decreased at P40, with no significant difference of P0 mRNA levels between the different PNS portions at each age studied. These findings demonstrate the lack of proximo-distal gradient in abundance of P0 mRNA during rat PNS development and add further insight in understanding the dynamics of axonal regulation of myelination in the PNS.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 285-288 |
| Number of pages | 4 |
| Journal | Developmental Brain Research |
| Volume | 83 |
| Issue number | 2 |
| DOIs | |
| State | Published - Dec 16 1994 |
| Externally published | Yes |
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Keywords
- Development
- In situ hybridization
- P0 protein mRNA
- Sciatic nerve
- Spinal root
ASJC Scopus subject areas
- Developmental Biology
- Developmental Neuroscience
Cite this
Expression of P0 protein mRNA along rat sciatic nerve during development. / Baron, Pierluigi; Shy, Michael; Kamholz, John; Scarlato, Guglielmo; Pleasure, David E.
In: Developmental Brain Research, Vol. 83, No. 2, 16.12.1994, p. 285-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of P0 protein mRNA along rat sciatic nerve during development
AU - Baron, Pierluigi
AU - Shy, Michael
AU - Kamholz, John
AU - Scarlato, Guglielmo
AU - Pleasure, David E
PY - 1994/12/16
Y1 - 1994/12/16
N2 - The temporal and spatial expression of P0 protein mRNA in the spinal nerve roots and in distal (popliteal trifurcation) segments of the developing rat sciatic nerve was examined using in situ hybridization. P0 mRNA was undetectable in the PNS portions at day 16 post-fertilization (E16) but present in the roots as well as in the sciatic nerve by E18. While Schwann cells were labelled by in situ hybridization at E18, it was not until birth (P1) that cells revealed the typical clusters of silver grains in a perinuclear distribution. Hybridization signal rose between P5 and P10, than decreased at P40, with no significant difference of P0 mRNA levels between the different PNS portions at each age studied. These findings demonstrate the lack of proximo-distal gradient in abundance of P0 mRNA during rat PNS development and add further insight in understanding the dynamics of axonal regulation of myelination in the PNS.
AB - The temporal and spatial expression of P0 protein mRNA in the spinal nerve roots and in distal (popliteal trifurcation) segments of the developing rat sciatic nerve was examined using in situ hybridization. P0 mRNA was undetectable in the PNS portions at day 16 post-fertilization (E16) but present in the roots as well as in the sciatic nerve by E18. While Schwann cells were labelled by in situ hybridization at E18, it was not until birth (P1) that cells revealed the typical clusters of silver grains in a perinuclear distribution. Hybridization signal rose between P5 and P10, than decreased at P40, with no significant difference of P0 mRNA levels between the different PNS portions at each age studied. These findings demonstrate the lack of proximo-distal gradient in abundance of P0 mRNA during rat PNS development and add further insight in understanding the dynamics of axonal regulation of myelination in the PNS.
KW - Development
KW - In situ hybridization
KW - P0 protein mRNA
KW - Sciatic nerve
KW - Spinal root
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UR - http://www.scopus.com/inward/citedby.url?scp=0028172416&partnerID=8YFLogxK
U2 - 10.1016/0165-3806(94)00149-9
DO - 10.1016/0165-3806(94)00149-9
M3 - Article
C2 - 7535206
AN - SCOPUS:0028172416
VL - 83
SP - 285
EP - 288
JO - Developmental Brain Research
JF - Developmental Brain Research
SN - 0165-3806
IS - 2
ER -