Expression of N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor genes in neuroblastoma, medulloblastoma, and other cell lines

Akira Yoshioka, Naohiko Ikegaki, Margaret Williams, David E Pleasure

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We evaluated expression of N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor (GluR) genes by reverse transcriptase-polymerase chain reaction (RT-PCR) and Southern blotting in nine established cell lines: rat CG-4 (oligodendroglial lineage) and RINm5F insulinoma cells; human CHP134, SMS-KCNR, SKNSH, and Nb69 neuroblastoma cells; and human D384Med, D425Med, and D458Med medulloblastoma cells. CG-4 expressed mRNAs encoding GluR2-7, KA- 1, and KA-2 non-NMDA GluR (Yoshioka et al.: J Neurochem 64:24422448, 1995) and NR1 (NMDAR1) and NR2D NMDA GluR. After differentiation to oligodendrocyte-like cells, CG-4 also expressed NR2B mRNA. Rat insulinoma cells expressed GluR5 and KA-2 non-NMDA and NR1 and NR2D NMDA GluR mRNAs. The four human neuroblastoma lines all expressed mRNAs encoding GluR2-4, 6, 7 and KA-1 non-NMDA and NR1 NMDA GluR, and the three human medulloblastoma cell lines all expressed mRNAs encoding GluR1, 6 and KA-1, but none of the NMDA GluRs. Whereas CG-4 is susceptible to kainate excitotoxicity, treatment of insulinoma, neuroblastoma, and medulloblastoma lines with L-glutamate, kainate, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA), or NMDA failed to cause cell damage or to augment 45Ca2+ influx. Thus, despite expressing a variety of non-NMDA and NMDA GluR genes, the human neuroblastoma and medulloblastoma and rat insulinoma lines failed to assemble Ca2+- permeable NMDA or non-NMDA GluR channels. This failure confers protection against excitotoxicity and may contribute to progression of tumors of these types.

Original languageEnglish (US)
Pages (from-to)164-172
Number of pages9
JournalJournal of Neuroscience Research
Volume46
Issue number2
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

D-Aspartic Acid
Medulloblastoma
Glutamate Receptors
N-Methylaspartate
Neuroblastoma
Insulinoma
Cell Line
N-Methyl-D-Aspartate Receptors
Genes
Messenger RNA
Kainic Acid
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Oligodendroglia
Southern Blotting
aspartic acid receptor
Reverse Transcriptase Polymerase Chain Reaction
Glutamic Acid
N-methylglutamate

Keywords

  • Ca influx
  • excitotoxicity
  • insulinoma
  • oligodendroglia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of N-methyl-D-aspartate (NMDA) and non-NMDA glutamate receptor genes in neuroblastoma, medulloblastoma, and other cell lines. / Yoshioka, Akira; Ikegaki, Naohiko; Williams, Margaret; Pleasure, David E.

In: Journal of Neuroscience Research, Vol. 46, No. 2, 1996, p. 164-172.

Research output: Contribution to journalArticle

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