Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer

Yue Wang, Vera S Go, James F. Holland, Stella M. Melana, Beatriz G T Pogo

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

We have reported previously (Wang et al., Cancer Res., 55: 5173-5179, 1995) the presence of a 660-bp sequence that is 90-98% homologous to the env gene of mouse mammary tumor virus in 38% of 314 unselected breast cancer samples, in some breast cancer cell lines, and in 1.8% of the 107 normal breast specimens from reduction mammoplasties. In this communication, we have investigated whether the 660-bp sequence or a smaller 256-bp sequence within the 660 bp were expressed in breast tumors, normal breasts, and breast cancer cell lines by means of reverse transcription-PCR. The results indicated that expression of the 660-bp sequence was detected in 66% of the sequence- positive breast tumors studied and in none of the negative tumors or normal breasts and that the 256-bp sequence was expressed in all of the env-positive breast cancer cell lines tested. Sequence analysis revealed that the expressed 660-bp sequence is 98% homologous to the env gene of mouse mammary tumor virus with only one small stretch of 46 bp homologous to endogenous viral sequences. The presence and expression of this sequence is related to a group of human breast cancers in which the concept of a retroviral etiology can be entertained.

Original languageEnglish (US)
Pages (from-to)2565-2568
Number of pages4
JournalClinical Cancer Research
Volume4
Issue number10
StatePublished - Oct 1998
Externally publishedYes

Fingerprint

env Genes
Mouse mammary tumor virus
Breast Neoplasms
Breast
Cell Line
Mammaplasty
Reverse Transcription
Sequence Analysis
Neoplasms
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wang, Y., Go, V. S., Holland, J. F., Melana, S. M., & Pogo, B. G. T. (1998). Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer. Clinical Cancer Research, 4(10), 2565-2568.

Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer. / Wang, Yue; Go, Vera S; Holland, James F.; Melana, Stella M.; Pogo, Beatriz G T.

In: Clinical Cancer Research, Vol. 4, No. 10, 10.1998, p. 2565-2568.

Research output: Contribution to journalArticle

Wang, Y, Go, VS, Holland, JF, Melana, SM & Pogo, BGT 1998, 'Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer', Clinical Cancer Research, vol. 4, no. 10, pp. 2565-2568.
Wang, Yue ; Go, Vera S ; Holland, James F. ; Melana, Stella M. ; Pogo, Beatriz G T. / Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer. In: Clinical Cancer Research. 1998 ; Vol. 4, No. 10. pp. 2565-2568.
@article{29a372385151430b9657b390712d46fc,
title = "Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer",
abstract = "We have reported previously (Wang et al., Cancer Res., 55: 5173-5179, 1995) the presence of a 660-bp sequence that is 90-98{\%} homologous to the env gene of mouse mammary tumor virus in 38{\%} of 314 unselected breast cancer samples, in some breast cancer cell lines, and in 1.8{\%} of the 107 normal breast specimens from reduction mammoplasties. In this communication, we have investigated whether the 660-bp sequence or a smaller 256-bp sequence within the 660 bp were expressed in breast tumors, normal breasts, and breast cancer cell lines by means of reverse transcription-PCR. The results indicated that expression of the 660-bp sequence was detected in 66{\%} of the sequence- positive breast tumors studied and in none of the negative tumors or normal breasts and that the 256-bp sequence was expressed in all of the env-positive breast cancer cell lines tested. Sequence analysis revealed that the expressed 660-bp sequence is 98{\%} homologous to the env gene of mouse mammary tumor virus with only one small stretch of 46 bp homologous to endogenous viral sequences. The presence and expression of this sequence is related to a group of human breast cancers in which the concept of a retroviral etiology can be entertained.",
author = "Yue Wang and Go, {Vera S} and Holland, {James F.} and Melana, {Stella M.} and Pogo, {Beatriz G T}",
year = "1998",
month = "10",
language = "English (US)",
volume = "4",
pages = "2565--2568",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "10",

}

TY - JOUR

T1 - Expression of mouse mammary tumor virus-like env gene sequences in human breast cancer

AU - Wang, Yue

AU - Go, Vera S

AU - Holland, James F.

AU - Melana, Stella M.

AU - Pogo, Beatriz G T

PY - 1998/10

Y1 - 1998/10

N2 - We have reported previously (Wang et al., Cancer Res., 55: 5173-5179, 1995) the presence of a 660-bp sequence that is 90-98% homologous to the env gene of mouse mammary tumor virus in 38% of 314 unselected breast cancer samples, in some breast cancer cell lines, and in 1.8% of the 107 normal breast specimens from reduction mammoplasties. In this communication, we have investigated whether the 660-bp sequence or a smaller 256-bp sequence within the 660 bp were expressed in breast tumors, normal breasts, and breast cancer cell lines by means of reverse transcription-PCR. The results indicated that expression of the 660-bp sequence was detected in 66% of the sequence- positive breast tumors studied and in none of the negative tumors or normal breasts and that the 256-bp sequence was expressed in all of the env-positive breast cancer cell lines tested. Sequence analysis revealed that the expressed 660-bp sequence is 98% homologous to the env gene of mouse mammary tumor virus with only one small stretch of 46 bp homologous to endogenous viral sequences. The presence and expression of this sequence is related to a group of human breast cancers in which the concept of a retroviral etiology can be entertained.

AB - We have reported previously (Wang et al., Cancer Res., 55: 5173-5179, 1995) the presence of a 660-bp sequence that is 90-98% homologous to the env gene of mouse mammary tumor virus in 38% of 314 unselected breast cancer samples, in some breast cancer cell lines, and in 1.8% of the 107 normal breast specimens from reduction mammoplasties. In this communication, we have investigated whether the 660-bp sequence or a smaller 256-bp sequence within the 660 bp were expressed in breast tumors, normal breasts, and breast cancer cell lines by means of reverse transcription-PCR. The results indicated that expression of the 660-bp sequence was detected in 66% of the sequence- positive breast tumors studied and in none of the negative tumors or normal breasts and that the 256-bp sequence was expressed in all of the env-positive breast cancer cell lines tested. Sequence analysis revealed that the expressed 660-bp sequence is 98% homologous to the env gene of mouse mammary tumor virus with only one small stretch of 46 bp homologous to endogenous viral sequences. The presence and expression of this sequence is related to a group of human breast cancers in which the concept of a retroviral etiology can be entertained.

UR - http://www.scopus.com/inward/record.url?scp=0031756295&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031756295&partnerID=8YFLogxK

M3 - Article

C2 - 9796992

AN - SCOPUS:0031756295

VL - 4

SP - 2565

EP - 2568

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 10

ER -