Expression of matrix proteins in an in vitro model of airway remodeling in asthma

Angelie R. Agarwal, Justin Mih, Steven George

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Acute asthma is characterized by a decrease in the pH of the exhaled breath condensate and bronchoconstriction. These perturbations may injure the epithelium in a chronic, intermittent pattern, leading to subepithelial fibrosis. We used an in vitro three-dimensional model of the bronchial mucosa to elucidate the response to a repeated chemical or physical insult to the epithelium in the postcontraction phase. We used enzyme-linked immunosorbent assay and reverse transcriptase - polymerase chain reaction to assess the production of the following proteins: matrix metalloproteinase (MMP) 3, MMP-9, tissue inhibitor of MMP-1, transforming growth factor β1, thrombospondin 1, tenascin, and fibronectin. The presence of the epithelium enhanced the degree of tissue contraction (50.1 ± 4.4% of original area versus 75.4 ± 2.3%). In the absence of injury, tenascin, fibronectin, MMP-3, and tissue inhibitor of MMP-1 are actively expressed. However, the chronic chemical wound markedly inhibited the expression of all proteins. We conclude that the epithelium, wound type, and age of the tissue (contracting versus postcontraction) impact the expression of key proteins in an in vitro model of subepithelial fibrosis in asthma.

Original languageEnglish (US)
Pages (from-to)35-42
Number of pages8
JournalAllergy and Asthma Proceedings
Volume24
Issue number1
StatePublished - Jan 1 2003

Fingerprint

Airway Remodeling
Asthma
Epithelium
Tenascin
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Fibronectins
Wounds and Injuries
Proteins
Fibrosis
Thrombospondin 1
Matrix Metalloproteinase Inhibitors
Bronchoconstriction
Matrix Metalloproteinase 9
Transforming Growth Factors
Reverse Transcriptase Polymerase Chain Reaction
Mucous Membrane
Enzyme-Linked Immunosorbent Assay
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pulmonary and Respiratory Medicine

Cite this

Expression of matrix proteins in an in vitro model of airway remodeling in asthma. / Agarwal, Angelie R.; Mih, Justin; George, Steven.

In: Allergy and Asthma Proceedings, Vol. 24, No. 1, 01.01.2003, p. 35-42.

Research output: Contribution to journalArticle

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