Abstract
Acute asthma is characterized by a decrease in the pH of the exhaled breath condensate and bronchoconstriction. These perturbations may injure the epithelium in a chronic, intermittent pattern, leading to subepithelial fibrosis. We used an in vitro three-dimensional model of the bronchial mucosa to elucidate the response to a repeated chemical or physical insult to the epithelium in the postcontraction phase. We used enzyme-linked immunosorbent assay and reverse transcriptase - polymerase chain reaction to assess the production of the following proteins: matrix metalloproteinase (MMP) 3, MMP-9, tissue inhibitor of MMP-1, transforming growth factor β1, thrombospondin 1, tenascin, and fibronectin. The presence of the epithelium enhanced the degree of tissue contraction (50.1 ± 4.4% of original area versus 75.4 ± 2.3%). In the absence of injury, tenascin, fibronectin, MMP-3, and tissue inhibitor of MMP-1 are actively expressed. However, the chronic chemical wound markedly inhibited the expression of all proteins. We conclude that the epithelium, wound type, and age of the tissue (contracting versus postcontraction) impact the expression of key proteins in an in vitro model of subepithelial fibrosis in asthma.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 35-42 |
| Number of pages | 8 |
| Journal | Allergy and Asthma Proceedings |
| Volume | 24 |
| Issue number | 1 |
| State | Published - Jan 1 2003 |
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ASJC Scopus subject areas
- Immunology and Allergy
- Pulmonary and Respiratory Medicine
Cite this
Expression of matrix proteins in an in vitro model of airway remodeling in asthma. / Agarwal, Angelie R.; Mih, Justin; George, Steven.
In: Allergy and Asthma Proceedings, Vol. 24, No. 1, 01.01.2003, p. 35-42.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of matrix proteins in an in vitro model of airway remodeling in asthma
AU - Agarwal, Angelie R.
AU - Mih, Justin
AU - George, Steven
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Acute asthma is characterized by a decrease in the pH of the exhaled breath condensate and bronchoconstriction. These perturbations may injure the epithelium in a chronic, intermittent pattern, leading to subepithelial fibrosis. We used an in vitro three-dimensional model of the bronchial mucosa to elucidate the response to a repeated chemical or physical insult to the epithelium in the postcontraction phase. We used enzyme-linked immunosorbent assay and reverse transcriptase - polymerase chain reaction to assess the production of the following proteins: matrix metalloproteinase (MMP) 3, MMP-9, tissue inhibitor of MMP-1, transforming growth factor β1, thrombospondin 1, tenascin, and fibronectin. The presence of the epithelium enhanced the degree of tissue contraction (50.1 ± 4.4% of original area versus 75.4 ± 2.3%). In the absence of injury, tenascin, fibronectin, MMP-3, and tissue inhibitor of MMP-1 are actively expressed. However, the chronic chemical wound markedly inhibited the expression of all proteins. We conclude that the epithelium, wound type, and age of the tissue (contracting versus postcontraction) impact the expression of key proteins in an in vitro model of subepithelial fibrosis in asthma.
AB - Acute asthma is characterized by a decrease in the pH of the exhaled breath condensate and bronchoconstriction. These perturbations may injure the epithelium in a chronic, intermittent pattern, leading to subepithelial fibrosis. We used an in vitro three-dimensional model of the bronchial mucosa to elucidate the response to a repeated chemical or physical insult to the epithelium in the postcontraction phase. We used enzyme-linked immunosorbent assay and reverse transcriptase - polymerase chain reaction to assess the production of the following proteins: matrix metalloproteinase (MMP) 3, MMP-9, tissue inhibitor of MMP-1, transforming growth factor β1, thrombospondin 1, tenascin, and fibronectin. The presence of the epithelium enhanced the degree of tissue contraction (50.1 ± 4.4% of original area versus 75.4 ± 2.3%). In the absence of injury, tenascin, fibronectin, MMP-3, and tissue inhibitor of MMP-1 are actively expressed. However, the chronic chemical wound markedly inhibited the expression of all proteins. We conclude that the epithelium, wound type, and age of the tissue (contracting versus postcontraction) impact the expression of key proteins in an in vitro model of subepithelial fibrosis in asthma.
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M3 - Article
C2 - 12635576
AN - SCOPUS:0037485896
VL - 24
SP - 35
EP - 42
JO - Allergy and Asthma Proceedings
JF - Allergy and Asthma Proceedings
SN - 1088-5412
IS - 1
ER -