Expression of Kv2.1 delayed rectifier K+ channel isoforms in the developing rat brain

James Trimmer

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Mammalian central neurons regulate a diverse set of functions by changes in spatial and temporal expression patterns of a large family of K+ channel gene products. Here the temporal patterns of expression of Kv2.1 (drkl) K+ channel mRNAs and polypeptides have been studied in rat brain. Northern blots using Kv2.1-specific probes show that three size classes (4.4, 9.0, 11.5 kb) of Kv2.1 K+ channel transcripts are present in rat brain. These mRNAs show differential patterns of expression over embryonic and postnatal developmental, with the 4.4 kb transcript being the predominant embryonic Kv2.1 mRNA, and the 11.5 kb transcript being the predominant adult mRNA. Immunoblots using Kv2.1-specific site-directed antibodies show the existence of multiple Kv2.1 polypeptides, which differ in their mobility on SDS polyacrylamide gels, in their immunoreactivity to a carboxyl terminal-directed anti-Kv2.1 antibody, and in their developmental expression. Thus Kv2.1 polypeptide isoforms exhibit discrete temporal patterning during neuronal development, implying distinct roles for these channel proteins in vivo.

Original languageEnglish (US)
Pages (from-to)205-210
Number of pages6
JournalFEBS Letters
Volume324
Issue number2
DOIs
StatePublished - Jun 14 1993
Externally publishedYes

Fingerprint

Rats
Brain
Protein Isoforms
Messenger RNA
Peptides
Antibodies
Northern Blotting
Neurons
Anti-Idiotypic Antibodies
Genes
Proteins

Keywords

  • Developmental expression
  • Immunoblotting
  • K channel
  • Northern blotting
  • Polypeptide isoform
  • Rat brain

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression of Kv2.1 delayed rectifier K+ channel isoforms in the developing rat brain. / Trimmer, James.

In: FEBS Letters, Vol. 324, No. 2, 14.06.1993, p. 205-210.

Research output: Contribution to journalArticle

@article{66009e0210e447be89eac8d5dd39b5ae,
title = "Expression of Kv2.1 delayed rectifier K+ channel isoforms in the developing rat brain",
abstract = "Mammalian central neurons regulate a diverse set of functions by changes in spatial and temporal expression patterns of a large family of K+ channel gene products. Here the temporal patterns of expression of Kv2.1 (drkl) K+ channel mRNAs and polypeptides have been studied in rat brain. Northern blots using Kv2.1-specific probes show that three size classes (4.4, 9.0, 11.5 kb) of Kv2.1 K+ channel transcripts are present in rat brain. These mRNAs show differential patterns of expression over embryonic and postnatal developmental, with the 4.4 kb transcript being the predominant embryonic Kv2.1 mRNA, and the 11.5 kb transcript being the predominant adult mRNA. Immunoblots using Kv2.1-specific site-directed antibodies show the existence of multiple Kv2.1 polypeptides, which differ in their mobility on SDS polyacrylamide gels, in their immunoreactivity to a carboxyl terminal-directed anti-Kv2.1 antibody, and in their developmental expression. Thus Kv2.1 polypeptide isoforms exhibit discrete temporal patterning during neuronal development, implying distinct roles for these channel proteins in vivo.",
keywords = "Developmental expression, Immunoblotting, K channel, Northern blotting, Polypeptide isoform, Rat brain",
author = "James Trimmer",
year = "1993",
month = "6",
day = "14",
doi = "10.1016/0014-5793(93)81394-F",
language = "English (US)",
volume = "324",
pages = "205--210",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Expression of Kv2.1 delayed rectifier K+ channel isoforms in the developing rat brain

AU - Trimmer, James

PY - 1993/6/14

Y1 - 1993/6/14

N2 - Mammalian central neurons regulate a diverse set of functions by changes in spatial and temporal expression patterns of a large family of K+ channel gene products. Here the temporal patterns of expression of Kv2.1 (drkl) K+ channel mRNAs and polypeptides have been studied in rat brain. Northern blots using Kv2.1-specific probes show that three size classes (4.4, 9.0, 11.5 kb) of Kv2.1 K+ channel transcripts are present in rat brain. These mRNAs show differential patterns of expression over embryonic and postnatal developmental, with the 4.4 kb transcript being the predominant embryonic Kv2.1 mRNA, and the 11.5 kb transcript being the predominant adult mRNA. Immunoblots using Kv2.1-specific site-directed antibodies show the existence of multiple Kv2.1 polypeptides, which differ in their mobility on SDS polyacrylamide gels, in their immunoreactivity to a carboxyl terminal-directed anti-Kv2.1 antibody, and in their developmental expression. Thus Kv2.1 polypeptide isoforms exhibit discrete temporal patterning during neuronal development, implying distinct roles for these channel proteins in vivo.

AB - Mammalian central neurons regulate a diverse set of functions by changes in spatial and temporal expression patterns of a large family of K+ channel gene products. Here the temporal patterns of expression of Kv2.1 (drkl) K+ channel mRNAs and polypeptides have been studied in rat brain. Northern blots using Kv2.1-specific probes show that three size classes (4.4, 9.0, 11.5 kb) of Kv2.1 K+ channel transcripts are present in rat brain. These mRNAs show differential patterns of expression over embryonic and postnatal developmental, with the 4.4 kb transcript being the predominant embryonic Kv2.1 mRNA, and the 11.5 kb transcript being the predominant adult mRNA. Immunoblots using Kv2.1-specific site-directed antibodies show the existence of multiple Kv2.1 polypeptides, which differ in their mobility on SDS polyacrylamide gels, in their immunoreactivity to a carboxyl terminal-directed anti-Kv2.1 antibody, and in their developmental expression. Thus Kv2.1 polypeptide isoforms exhibit discrete temporal patterning during neuronal development, implying distinct roles for these channel proteins in vivo.

KW - Developmental expression

KW - Immunoblotting

KW - K channel

KW - Northern blotting

KW - Polypeptide isoform

KW - Rat brain

UR - http://www.scopus.com/inward/record.url?scp=0027196945&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027196945&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(93)81394-F

DO - 10.1016/0014-5793(93)81394-F

M3 - Article

C2 - 8508921

AN - SCOPUS:0027196945

VL - 324

SP - 205

EP - 210

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -