Expression of interleukin-17B in mouse embryonic limb buds and regulation by BMP-7 and bFGF

Zongbing You, Grayson DuRaine, Janet Y L Tien, Corinne Lee, Timothy A. Moseley, A Hari Reddi

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Interleukin-17B (IL-17B) is a member of interleukin-17 family that displays a variety of proinflammatory and immune modulatory activities. In this study, we found that IL-17B mRNA was maximally expressed in the limb buds of 14.5 days post coitus (dpc) mouse embryo and declined to low level at 19.5 dpc. By immunohistochemical staining, the strongest IL-17B signals were observed in the cells of the bone collar in the primary ossification center. The chondrocytes in the resting and proliferative zones were stained moderately, while little staining was seen in the hypertrophic zone. Furthermore, in both C3H10T1/2 and MC3T3-E1 cells, the IL-17B mRNA was up-regulated by recombinant human bone morphogenetic protein-7, but down-regulated by basic fibroblast growth factor via the extracellular signal-regulated kinase pathway. This study provides the first evidence that IL-17B is expressed in the mouse embryonic limb buds and may play a role in chondrogenesis and osteogenesis.

Original languageEnglish (US)
Pages (from-to)624-631
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume326
Issue number3
DOIs
StatePublished - Jan 21 2005

Keywords

  • Bone development
  • Bone morphogenetic protein
  • Fibroblast growth factor
  • Interleukin-17
  • Limb buds

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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