Expression of integrins to control migration direction of electrotaxis

Kan Zhu, Yoko Takada, Kenichi Nakajima, Yaohui Sun, Jianxin Jiang, Yan Zhang, Qunli Zeng, Yoshikazu Takada, Min Zhao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Proper control of cell migration is critically important in many biologic processes, such as wound healing, immune surveillance, and development. Much progress has been made in the initiation of cell migration; however, little is known about termination and sometimes directional reversal. During active cell migration, as in wound healing, development, and immune surveillance, the integrin expression profile undergoes drastic changes. Here, we uncovered the extensive regulatory and even opposing roles of integrins in directional cell migration in electric fields (EFs), a potentially important endogenous guidance mechanism. We established cell lines that stably express specific integrins and determined their responses to applied EFs with a high throughput screen. Expression of specific integrins drove cells to migrate to the cathode or to the anode or to lose migration direction. Cells expressing αMβ2, β1, α2, αIIbβ3, and α5 migrated to the cathode, whereas cells expressing β3, α6, and α9 migrated to the anode. Cells expressing α4, αV, and α6β4 lost directional electrotaxis. Manipulation of α9 molecules, one of the molecular directional switches, suggested that the intracellular domain is critical for the directional reversal. These data revealed an unreported role for integrins in controlling stop, go, and reversal activity of directional migration of mammalian cells in EFs, which might ensure that cells reach their final destination with well-controlled speed and direction.-Zhu, K., Takada, Y., Nakajima, K., Sun, Y., Jiang, J., Zhang, Y., Zeng, Q., Takada, Y., Zhao, M. Expression of integrins to control migration direction of electrotaxis.

Original languageEnglish (US)
Pages (from-to)9131-9141
Number of pages11
JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Volume33
Issue number8
DOIs
StatePublished - Aug 1 2019

Fingerprint

Integrins
Cell Movement
Cells
Electrodes
Electric fields
Wound Healing
Anodes
Cathodes
Solar System
Sun
Direction compound
Switches
Throughput
Cell Line
Molecules

Keywords

  • directional cell migration
  • directional reversal
  • galvanotaxis
  • motility
  • α9

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Expression of integrins to control migration direction of electrotaxis. / Zhu, Kan; Takada, Yoko; Nakajima, Kenichi; Sun, Yaohui; Jiang, Jianxin; Zhang, Yan; Zeng, Qunli; Takada, Yoshikazu; Zhao, Min.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 8, 01.08.2019, p. 9131-9141.

Research output: Contribution to journalArticle

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