Expression of human lactoferrin in transgenic rice grains for the application in infant formula

Somen Nandi, Yasushi A. Suzuki, Jianmin Huang, Doris Yalda, Phuong Pham, Liying Wu, Glen Bartley, Ning Huang, Bo Lönnerdal

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Breast-fed infants are healthier than formula-fed infants, which may in part be due to the lack of components like human lactoferrin (HLF) in infant formula. Thus, fortification of infant formula with bioactive proteins such as recombinant HLF (rHLF) may provide health benefits. A synthetic HLF gene linked to a rice glutelin 1 promoter and signal sequence was transformed to rice cells to produce rHLF. The expression level was up to 0.5% of dehusked rice grain weight. The rHLF was specifically expressed in the rice grain but not in other tissues. Rice grains expressing rHLF were advanced for four generations and expression levels were stable. Biochemical and physical characterization showed that rHLF is identical to HLF in N-terminal sequence, pI, iron-binding capacity, antimicrobial activity against a human pathogen and resistance to protease digestion. Receptor binding activity assessed by Caco-2 cells, a human small intestinal cell line, was also retained for rHLF. Since rice may be used in infant formula, it is feasible that rice grains expressing rHLF can be directly incorporated into infant formula without purification of the rHLF, providing a convenient advantage over other expression systems.

Original languageEnglish (US)
Pages (from-to)713-722
Number of pages10
JournalPlant Science
Volume163
Issue number4
DOIs
StatePublished - Oct 1 2002

Fingerprint

Infant Formula
Lactoferrin
lactoferrin
infant formulas
genetically modified organisms
rice
Glutens
Pathogens
Protein Sorting Signals
Purification
Peptide Hydrolases
Iron
Genes
Cells
Health
glutelins
Tissue
Caco-2 Cells
binding capacity
Insurance Benefits

Keywords

  • Gene expression
  • Infant formula
  • Recombinant human lactoferrin
  • Transgenic rice grains

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Nandi, S., Suzuki, Y. A., Huang, J., Yalda, D., Pham, P., Wu, L., ... Lönnerdal, B. (2002). Expression of human lactoferrin in transgenic rice grains for the application in infant formula. Plant Science, 163(4), 713-722. https://doi.org/10.1016/S0168-9452(02)00165-6

Expression of human lactoferrin in transgenic rice grains for the application in infant formula. / Nandi, Somen; Suzuki, Yasushi A.; Huang, Jianmin; Yalda, Doris; Pham, Phuong; Wu, Liying; Bartley, Glen; Huang, Ning; Lönnerdal, Bo.

In: Plant Science, Vol. 163, No. 4, 01.10.2002, p. 713-722.

Research output: Contribution to journalArticle

Nandi, S, Suzuki, YA, Huang, J, Yalda, D, Pham, P, Wu, L, Bartley, G, Huang, N & Lönnerdal, B 2002, 'Expression of human lactoferrin in transgenic rice grains for the application in infant formula', Plant Science, vol. 163, no. 4, pp. 713-722. https://doi.org/10.1016/S0168-9452(02)00165-6
Nandi, Somen ; Suzuki, Yasushi A. ; Huang, Jianmin ; Yalda, Doris ; Pham, Phuong ; Wu, Liying ; Bartley, Glen ; Huang, Ning ; Lönnerdal, Bo. / Expression of human lactoferrin in transgenic rice grains for the application in infant formula. In: Plant Science. 2002 ; Vol. 163, No. 4. pp. 713-722.
@article{4b0c0671eda04b1199de7a10bc67a152,
title = "Expression of human lactoferrin in transgenic rice grains for the application in infant formula",
abstract = "Breast-fed infants are healthier than formula-fed infants, which may in part be due to the lack of components like human lactoferrin (HLF) in infant formula. Thus, fortification of infant formula with bioactive proteins such as recombinant HLF (rHLF) may provide health benefits. A synthetic HLF gene linked to a rice glutelin 1 promoter and signal sequence was transformed to rice cells to produce rHLF. The expression level was up to 0.5{\%} of dehusked rice grain weight. The rHLF was specifically expressed in the rice grain but not in other tissues. Rice grains expressing rHLF were advanced for four generations and expression levels were stable. Biochemical and physical characterization showed that rHLF is identical to HLF in N-terminal sequence, pI, iron-binding capacity, antimicrobial activity against a human pathogen and resistance to protease digestion. Receptor binding activity assessed by Caco-2 cells, a human small intestinal cell line, was also retained for rHLF. Since rice may be used in infant formula, it is feasible that rice grains expressing rHLF can be directly incorporated into infant formula without purification of the rHLF, providing a convenient advantage over other expression systems.",
keywords = "Gene expression, Infant formula, Recombinant human lactoferrin, Transgenic rice grains",
author = "Somen Nandi and Suzuki, {Yasushi A.} and Jianmin Huang and Doris Yalda and Phuong Pham and Liying Wu and Glen Bartley and Ning Huang and Bo L{\"o}nnerdal",
year = "2002",
month = "10",
day = "1",
doi = "10.1016/S0168-9452(02)00165-6",
language = "English (US)",
volume = "163",
pages = "713--722",
journal = "Plant Science",
issn = "0168-9452",
publisher = "Elsevier Ireland Ltd",
number = "4",

}

TY - JOUR

T1 - Expression of human lactoferrin in transgenic rice grains for the application in infant formula

AU - Nandi, Somen

AU - Suzuki, Yasushi A.

AU - Huang, Jianmin

AU - Yalda, Doris

AU - Pham, Phuong

AU - Wu, Liying

AU - Bartley, Glen

AU - Huang, Ning

AU - Lönnerdal, Bo

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Breast-fed infants are healthier than formula-fed infants, which may in part be due to the lack of components like human lactoferrin (HLF) in infant formula. Thus, fortification of infant formula with bioactive proteins such as recombinant HLF (rHLF) may provide health benefits. A synthetic HLF gene linked to a rice glutelin 1 promoter and signal sequence was transformed to rice cells to produce rHLF. The expression level was up to 0.5% of dehusked rice grain weight. The rHLF was specifically expressed in the rice grain but not in other tissues. Rice grains expressing rHLF were advanced for four generations and expression levels were stable. Biochemical and physical characterization showed that rHLF is identical to HLF in N-terminal sequence, pI, iron-binding capacity, antimicrobial activity against a human pathogen and resistance to protease digestion. Receptor binding activity assessed by Caco-2 cells, a human small intestinal cell line, was also retained for rHLF. Since rice may be used in infant formula, it is feasible that rice grains expressing rHLF can be directly incorporated into infant formula without purification of the rHLF, providing a convenient advantage over other expression systems.

AB - Breast-fed infants are healthier than formula-fed infants, which may in part be due to the lack of components like human lactoferrin (HLF) in infant formula. Thus, fortification of infant formula with bioactive proteins such as recombinant HLF (rHLF) may provide health benefits. A synthetic HLF gene linked to a rice glutelin 1 promoter and signal sequence was transformed to rice cells to produce rHLF. The expression level was up to 0.5% of dehusked rice grain weight. The rHLF was specifically expressed in the rice grain but not in other tissues. Rice grains expressing rHLF were advanced for four generations and expression levels were stable. Biochemical and physical characterization showed that rHLF is identical to HLF in N-terminal sequence, pI, iron-binding capacity, antimicrobial activity against a human pathogen and resistance to protease digestion. Receptor binding activity assessed by Caco-2 cells, a human small intestinal cell line, was also retained for rHLF. Since rice may be used in infant formula, it is feasible that rice grains expressing rHLF can be directly incorporated into infant formula without purification of the rHLF, providing a convenient advantage over other expression systems.

KW - Gene expression

KW - Infant formula

KW - Recombinant human lactoferrin

KW - Transgenic rice grains

UR - http://www.scopus.com/inward/record.url?scp=0036806486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036806486&partnerID=8YFLogxK

U2 - 10.1016/S0168-9452(02)00165-6

DO - 10.1016/S0168-9452(02)00165-6

M3 - Article

AN - SCOPUS:0036806486

VL - 163

SP - 713

EP - 722

JO - Plant Science

JF - Plant Science

SN - 0168-9452

IS - 4

ER -