Expression of hsp70 mRNA is induced in the brain of transgenic mice overexpressing human CuZn-superoxide dismutase following transient global cerebral ischemia

Takeo Kondo; Kensuke Murakami; Jari Honkaniemi; Frank R Sharp; Charles J. Epstein; Pak H. Chan

doi:10.1016/0006-8993(96)00949-3

Expression of hsp70 mRNA is induced in the brain of transgenic mice overexpressing human CuZn-superoxide dismutase following transient global cerebral ischemia

Takeo Kondo, Kensuke Murakami, Jari Honkaniemi, Frank R Sharp, Charles J. Epstein, Pak H. Chan

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We examined hsp70 mRNA expression in the brains of transgenic (Tg) mice overexpressing CuZn-superoxide dismutase and in wild-type (Wt) littermates after 3 min of bilateral common carotid artery occlusion. Significant induction of hsp70 mRNA occurred in the hippocampus, the cortex, and other brain regions of the Tg mice 4 h after ischemia compared to the Wt mice. However, there was no histological damage in the brains of Tg and Wt mice as assessed by both Cresyl violet staining and by TUNEL staining for DNA fragmentation These data suggest that high levels of CuZn-superoxide dismutase activity increase hsp70 mRNA expression and that intense hsp70 mRNA expression does not predict neuronal damage, even in vulnerable hippocampal CA1 neurons.

Original language	English (US)
Pages (from-to)	321-326
Number of pages	6
Journal	Brain Research
Volume	737
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(96)00949-3
State	Published - Oct 21 1996
Externally published	Yes

Keywords

CuZn-superoxide dismutase
Heat shock protein
Hippocampal CA1 neuron
hsp70 mRNA
Oxygen-free radical
Transgenic mouse
Transient global cerebral ischemia

ASJC Scopus subject areas

Neuroscience(all)

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Expression of hsp70 mRNA is induced in the brain of transgenic mice overexpressing human CuZn-superoxide dismutase following transient global cerebral ischemia. / Kondo, Takeo; Murakami, Kensuke; Honkaniemi, Jari; Sharp, Frank R; Epstein, Charles J.; Chan, Pak H.

In: Brain Research, Vol. 737, No. 1-2, 21.10.1996, p. 321-326.

Research output: Contribution to journal › Article › peer-review

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title = "Expression of hsp70 mRNA is induced in the brain of transgenic mice overexpressing human CuZn-superoxide dismutase following transient global cerebral ischemia",

abstract = "We examined hsp70 mRNA expression in the brains of transgenic (Tg) mice overexpressing CuZn-superoxide dismutase and in wild-type (Wt) littermates after 3 min of bilateral common carotid artery occlusion. Significant induction of hsp70 mRNA occurred in the hippocampus, the cortex, and other brain regions of the Tg mice 4 h after ischemia compared to the Wt mice. However, there was no histological damage in the brains of Tg and Wt mice as assessed by both Cresyl violet staining and by TUNEL staining for DNA fragmentation These data suggest that high levels of CuZn-superoxide dismutase activity increase hsp70 mRNA expression and that intense hsp70 mRNA expression does not predict neuronal damage, even in vulnerable hippocampal CA1 neurons.",

keywords = "CuZn-superoxide dismutase, Heat shock protein, Hippocampal CA1 neuron, hsp70 mRNA, Oxygen-free radical, Transgenic mouse, Transient global cerebral ischemia",

author = "Takeo Kondo and Kensuke Murakami and Jari Honkaniemi and Sharp, {Frank R} and Epstein, {Charles J.} and Chan, {Pak H.}",

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AU - Kondo, Takeo

AU - Murakami, Kensuke

AU - Honkaniemi, Jari

AU - Sharp, Frank R

AU - Epstein, Charles J.

AU - Chan, Pak H.

PY - 1996/10/21

Y1 - 1996/10/21

N2 - We examined hsp70 mRNA expression in the brains of transgenic (Tg) mice overexpressing CuZn-superoxide dismutase and in wild-type (Wt) littermates after 3 min of bilateral common carotid artery occlusion. Significant induction of hsp70 mRNA occurred in the hippocampus, the cortex, and other brain regions of the Tg mice 4 h after ischemia compared to the Wt mice. However, there was no histological damage in the brains of Tg and Wt mice as assessed by both Cresyl violet staining and by TUNEL staining for DNA fragmentation These data suggest that high levels of CuZn-superoxide dismutase activity increase hsp70 mRNA expression and that intense hsp70 mRNA expression does not predict neuronal damage, even in vulnerable hippocampal CA1 neurons.

AB - We examined hsp70 mRNA expression in the brains of transgenic (Tg) mice overexpressing CuZn-superoxide dismutase and in wild-type (Wt) littermates after 3 min of bilateral common carotid artery occlusion. Significant induction of hsp70 mRNA occurred in the hippocampus, the cortex, and other brain regions of the Tg mice 4 h after ischemia compared to the Wt mice. However, there was no histological damage in the brains of Tg and Wt mice as assessed by both Cresyl violet staining and by TUNEL staining for DNA fragmentation These data suggest that high levels of CuZn-superoxide dismutase activity increase hsp70 mRNA expression and that intense hsp70 mRNA expression does not predict neuronal damage, even in vulnerable hippocampal CA1 neurons.

KW - CuZn-superoxide dismutase

KW - Heat shock protein

KW - Hippocampal CA1 neuron

KW - hsp70 mRNA

KW - Oxygen-free radical

KW - Transgenic mouse

KW - Transient global cerebral ischemia

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Expression of hsp70 mRNA is induced in the brain of transgenic mice overexpressing human CuZn-superoxide dismutase following transient global cerebral ischemia

Abstract

Keywords

ASJC Scopus subject areas

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