Expression of FGF5 in choroidal neovascular membranes associated with ARMD

Takashi Kitaoka, Lawrence S Morse, Simon Schneeberger, Hitoshi Ishigooka, Leonard M Hjelmeland

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Purpose. The fibroblast growth factors (FGFs) are a family of 9 heparin binding proteins which have been proposed to play key roles in angiogenesis. Basic FGF (bFGF), acidic FGF (aFGF) and FGF5 have previously been demonstrated to be expressed in the normal retina and RPE. In this study, the expression of FGF5 was explored in choroidal neovascular membranes removed from patients with age-related macular degeneration (ARMD). Method. Three membranes were surgically removed from patients with ARMD, and were fixed, embedded and sectioned for immunohistochemistry. The membranes were immunostained with an affinity purified rabbit polyclonal antibody raised against the amino acid sequence for residues 175 to 185 of human FGF5 and visualized with the silver enhanced colloidal gold method for light microscopy. Results. FGF5 was expressed in membranes arising from ARMD, and was found primarily in blood vessels and the surrounding extracellular matrix. Conclusions. These results suggest that FGF5 may have a functional role in the pathophysiology of ARMD.

Original languageEnglish (US)
Pages (from-to)396-399
Number of pages4
JournalCurrent Eye Research
Volume16
Issue number4
StatePublished - 1997

Fingerprint

Macular Degeneration
Membranes
Fibroblast Growth Factor 1
Gold Colloid
Fibroblast Growth Factors
Fibroblast Growth Factor 2
Silver
Extracellular Matrix
Blood Vessels
Retina
Microscopy
Amino Acid Sequence
Immunohistochemistry
Rabbits
Light
Antibodies

Keywords

  • Age-related macular degeneration (ARMD)
  • Choroidal neovascularization
  • Fibroblast growth factors
  • Human
  • Immumohistochemistry

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Expression of FGF5 in choroidal neovascular membranes associated with ARMD. / Kitaoka, Takashi; Morse, Lawrence S; Schneeberger, Simon; Ishigooka, Hitoshi; Hjelmeland, Leonard M.

In: Current Eye Research, Vol. 16, No. 4, 1997, p. 396-399.

Research output: Contribution to journalArticle

Kitaoka, T, Morse, LS, Schneeberger, S, Ishigooka, H & Hjelmeland, LM 1997, 'Expression of FGF5 in choroidal neovascular membranes associated with ARMD', Current Eye Research, vol. 16, no. 4, pp. 396-399.
Kitaoka, Takashi ; Morse, Lawrence S ; Schneeberger, Simon ; Ishigooka, Hitoshi ; Hjelmeland, Leonard M. / Expression of FGF5 in choroidal neovascular membranes associated with ARMD. In: Current Eye Research. 1997 ; Vol. 16, No. 4. pp. 396-399.
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AU - Schneeberger, Simon

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AU - Hjelmeland, Leonard M

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AB - Purpose. The fibroblast growth factors (FGFs) are a family of 9 heparin binding proteins which have been proposed to play key roles in angiogenesis. Basic FGF (bFGF), acidic FGF (aFGF) and FGF5 have previously been demonstrated to be expressed in the normal retina and RPE. In this study, the expression of FGF5 was explored in choroidal neovascular membranes removed from patients with age-related macular degeneration (ARMD). Method. Three membranes were surgically removed from patients with ARMD, and were fixed, embedded and sectioned for immunohistochemistry. The membranes were immunostained with an affinity purified rabbit polyclonal antibody raised against the amino acid sequence for residues 175 to 185 of human FGF5 and visualized with the silver enhanced colloidal gold method for light microscopy. Results. FGF5 was expressed in membranes arising from ARMD, and was found primarily in blood vessels and the surrounding extracellular matrix. Conclusions. These results suggest that FGF5 may have a functional role in the pathophysiology of ARMD.

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