Abstract
Epstein-Barr virus (EBV) has 3 latent membrane proteins (LMPs) - LMP1, LMP2a, and LMP2b - which are expressed in nasopharyngeal carcinoma. Using keratinocyte cell lines expressing LMP2a and LMP2b and coexpressing LMP1/LMP2a, we grew organotypic raft cultures to analyze changes in morphology and expression of the cell adhesion molecule ICAM-1; α2, α3, α5, β1, and α6β4 integrins; laminin 5; E-cadherin; and desmoplakin. Cells expressing LMP2a or LMP2b were defective in their ability to mature and progress through normal squamous stratification when compared to the parental cell lines. Cells coexpressing LMP1/LMP2a additionally demonstrated 'pseudoinvasion' into the raft dermal equivalent. There was a consistent and dramatic up-regulation in the suprabasal expression of laminin 5 and α6β4 and β1 integrins in the LMP-expressing cell lines. ICAM-1, not expressed in the control cell lines, was up-regulated in the LMP-expressing cell lines. Expression of α3 and α5 integrins was also up-regulated in the LMP- expressing cell lines, while α2 demonstrated a loss of the normal basal layer expression. E-cadherin and desmoplakin expression patterns were essentially unchanged. We conclude that LMP2a and LMP2b singly, and LMP1/LMP2a coexpressed, are capable of altering keratinocyte cell adhesion molecule expression consistent with nasopharyngeal carcinoma.
Original language | English (US) |
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Pages (from-to) | 851-859 |
Number of pages | 9 |
Journal | Annals of Otology, Rhinology and Laryngology |
Volume | 108 |
Issue number | 9 I |
State | Published - 1999 |
Externally published | Yes |
Keywords
- Cell adhesion molecules
- Epstein-Barr virus
- Immunocytochemistry
- Latent membrane proteins
ASJC Scopus subject areas
- Otorhinolaryngology