Expression of cyclin D1/2 in the lungs of strain A/J mice fed chemopreventive agents

Hanspeter Witschi, Imelda Espiritu, Marie Suffia, Kent E Pinkerton

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Male strain A mice were fed a diet containing chemopreventive agents. After 1 and 3 weeks on the diets, lung nuclear fractions were examined for expression of cyclin D1/2 with western blot analysis. In animals fed a diet containing a mixture of myoinositol and dexamethasone, a treatment found previously to be effective in preventing the development of tobacco smoke-induced lung tumors in A/J mice, cyclin D1/2 expression was reduced to 30-40% of control levels. A similar decrease in cyclin D1/2 expression was found when animals were fed either myoinositol or dexamethasone alone. Paradoxically, tobacco smoke by itself had a similar effect on cyclin D1/2 expression. On the other hand, several agents that had been previously found not to be effective against tobacco smoke carcinogenesis [phenethyl isothiocyanate, 1,4-phenylenebis(methylene)selenoisocyanate, N-acetylcysteine, acetylsalicylic acid, D-limonene and beta carotene] did not decrease cyclin D1/2 expression after 1 or 3 weeks of feeding. It was concluded that expression of cyclin D1/2 might be a potentially useful marker in the identification of chemopreventive agents for tobacco smoke and could be of some help in the evaluation of their effects.

Original languageEnglish (US)
Pages (from-to)289-294
Number of pages6
JournalCarcinogenesis
Volume23
Issue number2
StatePublished - 2002

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Cyclin D1
Smoke
Lung
Tobacco
Inositol
Diet
Dexamethasone
beta Carotene
Acetylcysteine
Aspirin
Carcinogenesis
Western Blotting
Neoplasms

ASJC Scopus subject areas

  • Cancer Research

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Expression of cyclin D1/2 in the lungs of strain A/J mice fed chemopreventive agents. / Witschi, Hanspeter; Espiritu, Imelda; Suffia, Marie; Pinkerton, Kent E.

In: Carcinogenesis, Vol. 23, No. 2, 2002, p. 289-294.

Research output: Contribution to journalArticle

Witschi, Hanspeter ; Espiritu, Imelda ; Suffia, Marie ; Pinkerton, Kent E. / Expression of cyclin D1/2 in the lungs of strain A/J mice fed chemopreventive agents. In: Carcinogenesis. 2002 ; Vol. 23, No. 2. pp. 289-294.
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AU - Espiritu, Imelda

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AU - Pinkerton, Kent E

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