Abstract
CD40 is a 45 kDa cell surface glycoprotein of the tumor necrosis factor (TNF-alpha) gene family. Expression of CD40 has been reported on different hematopoeitic cell types. We observed that CD40 is also present on IL-3 dependent mast cell lines and bone marrow-derived mast cells (BMMC) by FACS analysis with MAb anti-CD40 (HM40-3). CD40 was upregulated on the surface of mast cells treated with PMA plus ionomycin, or incubated with GM-CSF and IFN-gamma. On the other hand, surface CD40 was also present on cells entering early or late phase of apoptosis, 24 hr following IL-3 withdrawal, as determined by uptake of annexin V and/or 7aaD, a viable dye. Recombinant CD40L further upregulated CD40 expression in IL-3 depleted cultures. Two molecular species of CD40 exhibiting Mr 40K and 45K were demonstrated by immunoblot in cell lysates prepared from IL-3 depleted cultures. These observations suggest that cell surface expression of CD40 may play a role in the maintenance of mast cells in vitro and/or their persistence in inflammatory infiltrates in vivo.
| Original language | English (US) |
|---|---|
| Journal | FASEB Journal |
| Volume | 12 |
| Issue number | 5 |
| State | Published - Mar 20 1998 |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
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Expression of CD40 on murine mast cells following IL-3 nutrient withdrawal. / Gong, J.; Liu, Fu-Tong; Chen, S. S.
In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of CD40 on murine mast cells following IL-3 nutrient withdrawal
AU - Gong, J.
AU - Liu, Fu-Tong
AU - Chen, S. S.
PY - 1998/3/20
Y1 - 1998/3/20
N2 - CD40 is a 45 kDa cell surface glycoprotein of the tumor necrosis factor (TNF-alpha) gene family. Expression of CD40 has been reported on different hematopoeitic cell types. We observed that CD40 is also present on IL-3 dependent mast cell lines and bone marrow-derived mast cells (BMMC) by FACS analysis with MAb anti-CD40 (HM40-3). CD40 was upregulated on the surface of mast cells treated with PMA plus ionomycin, or incubated with GM-CSF and IFN-gamma. On the other hand, surface CD40 was also present on cells entering early or late phase of apoptosis, 24 hr following IL-3 withdrawal, as determined by uptake of annexin V and/or 7aaD, a viable dye. Recombinant CD40L further upregulated CD40 expression in IL-3 depleted cultures. Two molecular species of CD40 exhibiting Mr 40K and 45K were demonstrated by immunoblot in cell lysates prepared from IL-3 depleted cultures. These observations suggest that cell surface expression of CD40 may play a role in the maintenance of mast cells in vitro and/or their persistence in inflammatory infiltrates in vivo.
AB - CD40 is a 45 kDa cell surface glycoprotein of the tumor necrosis factor (TNF-alpha) gene family. Expression of CD40 has been reported on different hematopoeitic cell types. We observed that CD40 is also present on IL-3 dependent mast cell lines and bone marrow-derived mast cells (BMMC) by FACS analysis with MAb anti-CD40 (HM40-3). CD40 was upregulated on the surface of mast cells treated with PMA plus ionomycin, or incubated with GM-CSF and IFN-gamma. On the other hand, surface CD40 was also present on cells entering early or late phase of apoptosis, 24 hr following IL-3 withdrawal, as determined by uptake of annexin V and/or 7aaD, a viable dye. Recombinant CD40L further upregulated CD40 expression in IL-3 depleted cultures. Two molecular species of CD40 exhibiting Mr 40K and 45K were demonstrated by immunoblot in cell lysates prepared from IL-3 depleted cultures. These observations suggest that cell surface expression of CD40 may play a role in the maintenance of mast cells in vitro and/or their persistence in inflammatory infiltrates in vivo.
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M3 - Article
AN - SCOPUS:33749296967
VL - 12
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 5
ER -