Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential

Jamal Qualai, Lin Xi Li, Jon Cantero, Antoni Tarrats, Marco Antonio Fernández, Lauro Sumoy, Annie Rodolosse, Stephen J Mcsorley, Meritxell Genescà

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

CD11c is an α integrin classically employed to define myeloid dendritic cells. Although there is little information about CD11c expression on human T cells, mouse models have shown an association of CD11c expression with functionally relevant T cell subsets. In the context of genital tract infection, we have previously observed increased expression of CD11c in circulating T cells from mice and women. Microarray analyses of activated effector T cells expressing CD11c derived from naïve mice demonstrated enrichment for natural killer (NK) associated genes. Here we find that murine CD11c+ T cells analyzed by flow cytometry display markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T (iNKT) cells. However, in women, only γδ T cells and CD8+ T cells were enriched within the CD11c fraction of blood and cervical tissue. These CD11c+ cells were highly activated and had greater interferon (IFN)-γ secretory capacity than CD11c- T cells. Furthermore, circulating CD11c+ T cells were associated with the expression of multiple adhesion molecules in women, suggesting that these cells have high tissue homing potential. These data suggest that CD11c expression distinguishes a population of circulating T cells during bacterial infection with innate capacity and mucosal homing potential.

Original languageEnglish (US)
Pages (from-to)e0154253
JournalPLoS One
Volume11
Issue number4
DOIs
StatePublished - 2016
Externally publishedYes

Fingerprint

T-cells
T-Lymphocyte Subsets
T-lymphocytes
T-Lymphocytes
Reproductive Tract Infections
mice
Natural Killer T-Cells
Tissue
Myeloid Cells
Microarray Analysis
Bacterial Infections
Integrins
Dendritic Cells
Interferons
Flow cytometry
integrins
natural killer cells
Flow Cytometry
interferons
dendritic cells

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Qualai, J., Li, L. X., Cantero, J., Tarrats, A., Fernández, M. A., Sumoy, L., ... Genescà, M. (2016). Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential. PLoS One, 11(4), e0154253. https://doi.org/10.1371/journal.pone.0154253

Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential. / Qualai, Jamal; Li, Lin Xi; Cantero, Jon; Tarrats, Antoni; Fernández, Marco Antonio; Sumoy, Lauro; Rodolosse, Annie; Mcsorley, Stephen J; Genescà, Meritxell.

In: PLoS One, Vol. 11, No. 4, 2016, p. e0154253.

Research output: Contribution to journalArticle

Qualai, J, Li, LX, Cantero, J, Tarrats, A, Fernández, MA, Sumoy, L, Rodolosse, A, Mcsorley, SJ & Genescà, M 2016, 'Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential', PLoS One, vol. 11, no. 4, pp. e0154253. https://doi.org/10.1371/journal.pone.0154253
Qualai, Jamal ; Li, Lin Xi ; Cantero, Jon ; Tarrats, Antoni ; Fernández, Marco Antonio ; Sumoy, Lauro ; Rodolosse, Annie ; Mcsorley, Stephen J ; Genescà, Meritxell. / Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential. In: PLoS One. 2016 ; Vol. 11, No. 4. pp. e0154253.
@article{0b86b04b1d8d4da98cc2bf731095a82a,
title = "Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential",
abstract = "CD11c is an α integrin classically employed to define myeloid dendritic cells. Although there is little information about CD11c expression on human T cells, mouse models have shown an association of CD11c expression with functionally relevant T cell subsets. In the context of genital tract infection, we have previously observed increased expression of CD11c in circulating T cells from mice and women. Microarray analyses of activated effector T cells expressing CD11c derived from na{\"i}ve mice demonstrated enrichment for natural killer (NK) associated genes. Here we find that murine CD11c+ T cells analyzed by flow cytometry display markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T (iNKT) cells. However, in women, only γδ T cells and CD8+ T cells were enriched within the CD11c fraction of blood and cervical tissue. These CD11c+ cells were highly activated and had greater interferon (IFN)-γ secretory capacity than CD11c- T cells. Furthermore, circulating CD11c+ T cells were associated with the expression of multiple adhesion molecules in women, suggesting that these cells have high tissue homing potential. These data suggest that CD11c expression distinguishes a population of circulating T cells during bacterial infection with innate capacity and mucosal homing potential.",
author = "Jamal Qualai and Li, {Lin Xi} and Jon Cantero and Antoni Tarrats and Fern{\'a}ndez, {Marco Antonio} and Lauro Sumoy and Annie Rodolosse and Mcsorley, {Stephen J} and Meritxell Genesc{\`a}",
year = "2016",
doi = "10.1371/journal.pone.0154253",
language = "English (US)",
volume = "11",
pages = "e0154253",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "4",

}

TY - JOUR

T1 - Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential

AU - Qualai, Jamal

AU - Li, Lin Xi

AU - Cantero, Jon

AU - Tarrats, Antoni

AU - Fernández, Marco Antonio

AU - Sumoy, Lauro

AU - Rodolosse, Annie

AU - Mcsorley, Stephen J

AU - Genescà, Meritxell

PY - 2016

Y1 - 2016

N2 - CD11c is an α integrin classically employed to define myeloid dendritic cells. Although there is little information about CD11c expression on human T cells, mouse models have shown an association of CD11c expression with functionally relevant T cell subsets. In the context of genital tract infection, we have previously observed increased expression of CD11c in circulating T cells from mice and women. Microarray analyses of activated effector T cells expressing CD11c derived from naïve mice demonstrated enrichment for natural killer (NK) associated genes. Here we find that murine CD11c+ T cells analyzed by flow cytometry display markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T (iNKT) cells. However, in women, only γδ T cells and CD8+ T cells were enriched within the CD11c fraction of blood and cervical tissue. These CD11c+ cells were highly activated and had greater interferon (IFN)-γ secretory capacity than CD11c- T cells. Furthermore, circulating CD11c+ T cells were associated with the expression of multiple adhesion molecules in women, suggesting that these cells have high tissue homing potential. These data suggest that CD11c expression distinguishes a population of circulating T cells during bacterial infection with innate capacity and mucosal homing potential.

AB - CD11c is an α integrin classically employed to define myeloid dendritic cells. Although there is little information about CD11c expression on human T cells, mouse models have shown an association of CD11c expression with functionally relevant T cell subsets. In the context of genital tract infection, we have previously observed increased expression of CD11c in circulating T cells from mice and women. Microarray analyses of activated effector T cells expressing CD11c derived from naïve mice demonstrated enrichment for natural killer (NK) associated genes. Here we find that murine CD11c+ T cells analyzed by flow cytometry display markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T (iNKT) cells. However, in women, only γδ T cells and CD8+ T cells were enriched within the CD11c fraction of blood and cervical tissue. These CD11c+ cells were highly activated and had greater interferon (IFN)-γ secretory capacity than CD11c- T cells. Furthermore, circulating CD11c+ T cells were associated with the expression of multiple adhesion molecules in women, suggesting that these cells have high tissue homing potential. These data suggest that CD11c expression distinguishes a population of circulating T cells during bacterial infection with innate capacity and mucosal homing potential.

UR - http://www.scopus.com/inward/record.url?scp=85015598556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015598556&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0154253

DO - 10.1371/journal.pone.0154253

M3 - Article

VL - 11

SP - e0154253

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 4

ER -