Expression of c-fos protein in brain: Metabolic mapping at the cellular level

S. M. Sagar, Frank R Sharp, T. Curran

Research output: Contribution to journalArticle

1689 Citations (Scopus)

Abstract

The proto-oncogene c-fos is expressed in neurons in response to direct stimulation by growth factors and neurotransmitters. In order to determine whether die c-fos protein (Fos) and Fos-related proteins can be induced in response to polysynaptic activation, rat hindlimb motor/sensory cortex was stimulated electrically and Fos expression examined immunohistochemically. Three hours after the onset of stimulation, focal nuclear Fos staining was seen in motor and sensory thalamus, pontine nuclei, globus pallidus, and cerebellum. Moreover, 24-hour water deprivation resulted in Fos expression in paraventricular and supraoptic nuclei. Fos immunohistochemistry therefore provides a cellular method to label polysynaptically activated neurons and thereby map functional pathways.

Original languageEnglish (US)
Pages (from-to)1328-1331
Number of pages4
JournalScience Science
Volume240
Issue number4857
StatePublished - 1988
Externally publishedYes

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Proto-Oncogene Proteins c-fos
Brain Mapping
Water Deprivation
fos Genes
Neurons
Supraoptic Nucleus
Globus Pallidus
Paraventricular Hypothalamic Nucleus
Hindlimb
Thalamus
Cerebellum
Neurotransmitter Agents
Intercellular Signaling Peptides and Proteins
Immunohistochemistry
Staining and Labeling
Proteins
Sensorimotor Cortex

ASJC Scopus subject areas

  • General

Cite this

Expression of c-fos protein in brain : Metabolic mapping at the cellular level. / Sagar, S. M.; Sharp, Frank R; Curran, T.

In: Science Science, Vol. 240, No. 4857, 1988, p. 1328-1331.

Research output: Contribution to journalArticle

Sagar, S. M. ; Sharp, Frank R ; Curran, T. / Expression of c-fos protein in brain : Metabolic mapping at the cellular level. In: Science Science. 1988 ; Vol. 240, No. 4857. pp. 1328-1331.
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