Abstract
The aim of this study was to determine the role of oxidative stress on c-fos and hsp70 gene expression in transgenic (Tg) mice overexpressing CuZn-superoxide dismutase (SOD-1) following traumatic brain injury (TBI). hsp70 mRNA, as investigated using in situ hybridization, was induced around the lesion at 4 and 24 h, but not at 1 and 48 h, in both Tg and non-transgenic (nTg) mice littermates. The degree of hsp70 induction was somewhat greater in nTg than Tg mice at 4 and 24 h after TBI. c-fos mRNA was induced throughout cortex, hippocampus, caudate putamen and the ventricular wall in Tg and nTg mice. TBI induced c-fos bilaterally in the cortex in both animals. There was a time-dependent differnce in cortical c-fos expression between nTg and Tg mice. The induction of c-fos mRNA in the was greater in nTg at 24 h and decreased in both animals by 48 h. Edema of the injured cortex was significantly attenuated in Tg mice at all time points (1-48 h). These data show that the degree of hsp70 induction and the degree, extent, and duration of c-fos induction produced by TBI are affected by levels of superoxide dismutase activity. It is proposed that superoxide radicals affect spreading depression and brain edema produced by TBI and that this may either directly or indirectly modlulate the expression of the c-fos and hsp70 genes after TBI.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 288-294 |
| Number of pages | 7 |
| Journal | Molecular Brain Research |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1995 |
| Externally published | Yes |
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Keywords
- c-fos mRNA
- Free radical
- hsp70 mRNA
- Spreading depression
- Superoxide dismutase
- Transgenic mouse
- Traumatic brain injury
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
Cite this
Expression of c-fos and hsp70 mRNA after traumatic brain injury in transgenic mice overexpressing CuZn-superoxide dismutase. / Mikawa, Shigeki; Sharp, Frank R; Kamii, Hideyuki; Kinouchi, Hiroyuki; Epstein, Charles J.; Chan, Pak H.
In: Molecular Brain Research, Vol. 33, No. 2, 1995, p. 288-294.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of c-fos and hsp70 mRNA after traumatic brain injury in transgenic mice overexpressing CuZn-superoxide dismutase
AU - Mikawa, Shigeki
AU - Sharp, Frank R
AU - Kamii, Hideyuki
AU - Kinouchi, Hiroyuki
AU - Epstein, Charles J.
AU - Chan, Pak H.
PY - 1995
Y1 - 1995
N2 - The aim of this study was to determine the role of oxidative stress on c-fos and hsp70 gene expression in transgenic (Tg) mice overexpressing CuZn-superoxide dismutase (SOD-1) following traumatic brain injury (TBI). hsp70 mRNA, as investigated using in situ hybridization, was induced around the lesion at 4 and 24 h, but not at 1 and 48 h, in both Tg and non-transgenic (nTg) mice littermates. The degree of hsp70 induction was somewhat greater in nTg than Tg mice at 4 and 24 h after TBI. c-fos mRNA was induced throughout cortex, hippocampus, caudate putamen and the ventricular wall in Tg and nTg mice. TBI induced c-fos bilaterally in the cortex in both animals. There was a time-dependent differnce in cortical c-fos expression between nTg and Tg mice. The induction of c-fos mRNA in the was greater in nTg at 24 h and decreased in both animals by 48 h. Edema of the injured cortex was significantly attenuated in Tg mice at all time points (1-48 h). These data show that the degree of hsp70 induction and the degree, extent, and duration of c-fos induction produced by TBI are affected by levels of superoxide dismutase activity. It is proposed that superoxide radicals affect spreading depression and brain edema produced by TBI and that this may either directly or indirectly modlulate the expression of the c-fos and hsp70 genes after TBI.
AB - The aim of this study was to determine the role of oxidative stress on c-fos and hsp70 gene expression in transgenic (Tg) mice overexpressing CuZn-superoxide dismutase (SOD-1) following traumatic brain injury (TBI). hsp70 mRNA, as investigated using in situ hybridization, was induced around the lesion at 4 and 24 h, but not at 1 and 48 h, in both Tg and non-transgenic (nTg) mice littermates. The degree of hsp70 induction was somewhat greater in nTg than Tg mice at 4 and 24 h after TBI. c-fos mRNA was induced throughout cortex, hippocampus, caudate putamen and the ventricular wall in Tg and nTg mice. TBI induced c-fos bilaterally in the cortex in both animals. There was a time-dependent differnce in cortical c-fos expression between nTg and Tg mice. The induction of c-fos mRNA in the was greater in nTg at 24 h and decreased in both animals by 48 h. Edema of the injured cortex was significantly attenuated in Tg mice at all time points (1-48 h). These data show that the degree of hsp70 induction and the degree, extent, and duration of c-fos induction produced by TBI are affected by levels of superoxide dismutase activity. It is proposed that superoxide radicals affect spreading depression and brain edema produced by TBI and that this may either directly or indirectly modlulate the expression of the c-fos and hsp70 genes after TBI.
KW - c-fos mRNA
KW - Free radical
KW - hsp70 mRNA
KW - Spreading depression
KW - Superoxide dismutase
KW - Transgenic mouse
KW - Traumatic brain injury
UR - http://www.scopus.com/inward/record.url?scp=0029151038&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029151038&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(95)00146-J
DO - 10.1016/0169-328X(95)00146-J
M3 - Article
C2 - 8750888
AN - SCOPUS:0029151038
VL - 33
SP - 288
EP - 294
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -