Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV

Pablo E. Piñeyro, Scott P. Kenney, Luis G. Giménez-Lirola, C. Lynn Heffron, Shannon R. Matzinger, Tanja Opriessnig, Xiang Jin Meng

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Co-infection of pigs in the field with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is common and poses a major concern in effective control of PCV2 and PRRSV. We previously demonstrated that insertion of foreign epitope tags in the C-terminus of PCV2 ORF2 produced infectious virions that elicited humoral immune responses against both PCV2 capsid and inserted epitope tags. In this study, we aimed to determine whether the non-pathogenic chimeric virus PCV1-2a, which is the basis for the licensed PCV2 vaccine Fostera™ PCV, can express PRRSV antigenic epitopes, thus generating dual immunity as a potential bivalent vaccine against both PCV2 and PPRSV. Four different linear B-cell antigenic epitopes of PRRSV were inserted into the C-terminus of the capsid gene of the PCV1-2a vaccine virus. We showed that insertion of 12 (PRRSV-GP2 epitope II, PRRSV-GP3 epitope I, and PRRSV-GP5 epitope I), and 14 (PRRSV-GP5 epitope IV) amino acid residues did not impair the replication of the resulting PCV1-2a-PRRSVEPI chimeric viruses in vitro. The four chimeric PCV1-2a viruses expressing PRRSV B-cell linear epitopes were successfully rescued and characterized. An immunogenicity study in pigs revealed that two of the four chimeric viruses, PCV1-2a-PRRSVEPIGP3IG and PCV1-2a-PRRSVEPIEPIGP5IV, elicited neutralizing antibodies against PRRSV VR2385 as well as PCV2 (strains PCV2a, PCV2b, and mPCV2b). The results have important implications for exploring the potential use of PCV1-2a vaccine virus as a live virus vector to develop bivalent MLVs against both PCV2 and PRRSV.

Original languageEnglish (US)
Pages (from-to)154-164
Number of pages11
JournalVirus Research
Volume210
DOIs
StatePublished - Dec 2 2015
Externally publishedYes

Fingerprint

Circovirus
Porcine respiratory and reproductive syndrome virus
Epitopes
Vaccines
Viruses
B-Lymphocyte Epitopes
Capsid
Swine
Humoral Immunity
Neutralizing Antibodies
Coinfection
Virion
Immunity

Keywords

  • Bivalent modified live-attenuated vaccine (MLV)
  • Chimeric PCV1-2 vaccine
  • Porcine circovirus type 2 (PCV2)
  • Porcine reproductive and respiratory syndrome virus (PRRSV)

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV. / Piñeyro, Pablo E.; Kenney, Scott P.; Giménez-Lirola, Luis G.; Heffron, C. Lynn; Matzinger, Shannon R.; Opriessnig, Tanja; Meng, Xiang Jin.

In: Virus Research, Vol. 210, 02.12.2015, p. 154-164.

Research output: Contribution to journalArticle

Piñeyro, Pablo E. ; Kenney, Scott P. ; Giménez-Lirola, Luis G. ; Heffron, C. Lynn ; Matzinger, Shannon R. ; Opriessnig, Tanja ; Meng, Xiang Jin. / Expression of antigenic epitopes of porcine reproductive and respiratory syndrome virus (PRRSV) in a modified live-attenuated porcine circovirus type 2 (PCV2) vaccine virus (PCV1-2a) as a potential bivalent vaccine against both PCV2 and PRRSV. In: Virus Research. 2015 ; Vol. 210. pp. 154-164.
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abstract = "Co-infection of pigs in the field with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is common and poses a major concern in effective control of PCV2 and PRRSV. We previously demonstrated that insertion of foreign epitope tags in the C-terminus of PCV2 ORF2 produced infectious virions that elicited humoral immune responses against both PCV2 capsid and inserted epitope tags. In this study, we aimed to determine whether the non-pathogenic chimeric virus PCV1-2a, which is the basis for the licensed PCV2 vaccine Fostera™ PCV, can express PRRSV antigenic epitopes, thus generating dual immunity as a potential bivalent vaccine against both PCV2 and PPRSV. Four different linear B-cell antigenic epitopes of PRRSV were inserted into the C-terminus of the capsid gene of the PCV1-2a vaccine virus. We showed that insertion of 12 (PRRSV-GP2 epitope II, PRRSV-GP3 epitope I, and PRRSV-GP5 epitope I), and 14 (PRRSV-GP5 epitope IV) amino acid residues did not impair the replication of the resulting PCV1-2a-PRRSVEPI chimeric viruses in vitro. The four chimeric PCV1-2a viruses expressing PRRSV B-cell linear epitopes were successfully rescued and characterized. An immunogenicity study in pigs revealed that two of the four chimeric viruses, PCV1-2a-PRRSVEPIGP3IG and PCV1-2a-PRRSVEPIEPIGP5IV, elicited neutralizing antibodies against PRRSV VR2385 as well as PCV2 (strains PCV2a, PCV2b, and mPCV2b). The results have important implications for exploring the potential use of PCV1-2a vaccine virus as a live virus vector to develop bivalent MLVs against both PCV2 and PRRSV.",
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AU - Giménez-Lirola, Luis G.

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AU - Opriessnig, Tanja

AU - Meng, Xiang Jin

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KW - Porcine reproductive and respiratory syndrome virus (PRRSV)

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