Expression of anti-Müllerian hormone, cyclin-dependent kinase inhibitor (CDKN1B), androgen receptor, and connexin 43 in equine testes during puberty

J. Almeida, Alan J Conley, L. Mathewson, B. A. Ball

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Sertoli cells are essential in development of a functional testis. During puberty, Sertoli cell maturation can be characterized by a number of markers, including anti-Müllerian hormone (AMH) and its receptor (AMHR2), androgen receptor (AR), cyclin-dependent kinase inhibitor (CDKN1B), and connexin 43 (Cx43). In the present study, immunohistochemistry (IHC) and real-time quantitative polymerase chain reaction (RT-qPCR) were used to characterize changes in expression of AMH, AMHR2, AR, CDKN1B, and Cx43 in prepubertal, postpubertal, and adult equine testes. During puberty, AMH expression decreased, and expression of AR as well as CDKN1B increased in Sertoli cells coinciding with the period of Sertoli cell maturation, arrest of cell proliferation, and presumptive AMH regulation by testosterone. Expression of AMHR2 appeared to decrease in Sertoli cells and increase in Leydig cells during pubertal maturation of the equine testis. In addition, expression and distribution of Cx43 changed during puberty in the stallion, suggesting a role for Cx43 in Sertoli cell signaling and maturation, hormone secretion, and blood-testis barrier formation. We concluded that Sertoli cell maturation during puberty in the stallion was accompanied by a reduced expression of AMH and its receptor, arrest of cell proliferation, increased expression of AR, and organization of gap-junctional communication.

Original languageEnglish (US)
Pages (from-to)847-857
Number of pages11
JournalTheriogenology
Volume77
Issue number5
DOIs
StatePublished - Mar 15 2012

Fingerprint

connexins
Connexin 43
cyclin-dependent kinase
Cyclin-Dependent Kinases
Sertoli Cells
Sertoli cells
Androgen Receptors
puberty
Puberty
Horses
Testis
testes
hormones
Hormones
horses
stallions
cell proliferation
Blood-Testis Barrier
Cell Proliferation
receptors

Keywords

  • Anti-Müllerian hormone
  • Horse
  • Puberty
  • Sertoli cell
  • Stallion
  • Testis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Equine
  • Food Animals
  • Small Animals

Cite this

Expression of anti-Müllerian hormone, cyclin-dependent kinase inhibitor (CDKN1B), androgen receptor, and connexin 43 in equine testes during puberty. / Almeida, J.; Conley, Alan J; Mathewson, L.; Ball, B. A.

In: Theriogenology, Vol. 77, No. 5, 15.03.2012, p. 847-857.

Research output: Contribution to journalArticle

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abstract = "Sertoli cells are essential in development of a functional testis. During puberty, Sertoli cell maturation can be characterized by a number of markers, including anti-M{\"u}llerian hormone (AMH) and its receptor (AMHR2), androgen receptor (AR), cyclin-dependent kinase inhibitor (CDKN1B), and connexin 43 (Cx43). In the present study, immunohistochemistry (IHC) and real-time quantitative polymerase chain reaction (RT-qPCR) were used to characterize changes in expression of AMH, AMHR2, AR, CDKN1B, and Cx43 in prepubertal, postpubertal, and adult equine testes. During puberty, AMH expression decreased, and expression of AR as well as CDKN1B increased in Sertoli cells coinciding with the period of Sertoli cell maturation, arrest of cell proliferation, and presumptive AMH regulation by testosterone. Expression of AMHR2 appeared to decrease in Sertoli cells and increase in Leydig cells during pubertal maturation of the equine testis. In addition, expression and distribution of Cx43 changed during puberty in the stallion, suggesting a role for Cx43 in Sertoli cell signaling and maturation, hormone secretion, and blood-testis barrier formation. We concluded that Sertoli cell maturation during puberty in the stallion was accompanied by a reduced expression of AMH and its receptor, arrest of cell proliferation, increased expression of AR, and organization of gap-junctional communication.",
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