Expression of anti-Müllerian hormone, CDKN1B, connexin 43, androgen receptor and steroidogenic enzymes in the equine cryptorchid testis

J. Almeida, Alan J Conley, B. A. Ball

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Reasons for performing study: Cryptorchidism affects 2-8% of male horses and the affected testis undergoes a disruption of normal spermatogenesis. The underlying molecular changes are poorly understood in the cryptorchid equine testis. Objectives: Compare the expression of anti-Müllerian hormone (AMH), anti-Müllerian hormone receptor (AMHR2), androgen receptor (AR), cyclin kinase inhibitor (CDKN1B), connexin 43 (Cx43), 3β hydroxysteroid dehydrogenase/Δ54- isomerase (3βHSD), P450c17 hydroxylase/lyase (P450c17) and cytochrome P450 aromatase (P450arom) in the undescended testis of cryptorchid stallions with that of normal stallions. Methods: Undescended, abdominal testes from four cryptorchid stallions between 2 and 3 years of age were collected during routine castrations along with normally descended testes from normal stallions between 2 and 3 years of age (n = 7). Samples were analysed by immunohistochemistry and quantitative real-time PCR. Results: Cryptorchid testes had increased AMH and AMHR2 immunolabelling when compared with normal testes, which indicates failure of maturation of Sertoli cells and/or lack of testosterone suppression. Failure of Sertoli cell maturation in the cryptorchid testis may also be attributed to AR abnormalities and/or a consequence of lack of testosterone suppression due to decreased 3βHSD. Cyclin-dependent kinase (CDKN1B) was not expressed in Sertoli cells of cryptorchid testes suggesting that Sertoli cells are still proliferating, which is also a characteristic of the immature testis. In addition, Cx43 expression is decreased in the cryptorchid testis, indicating a disruption in intercellular communication. Conclusions: Undescended testes of cryptorchid horses present characteristics of immaturity suggesting that the failure of Sertoli cell maturation may be a consequence of cryptorchidism. Potential relevance: This study provides a better understanding of the effect of cryptorchidism on testicular function in stallions.

Original languageEnglish (US)
Pages (from-to)538-545
Number of pages8
JournalEquine Veterinary Journal
Volume45
Issue number5
DOIs
StatePublished - Sep 2013

Fingerprint

connexins
Connexin 43
Androgen Receptors
Horses
Testis
testes
Cryptorchidism
hormones
Hormones
horses
Sertoli Cells
Enzymes
enzymes
Sertoli cells
stallions
cryptorchidism
3-Hydroxysteroid Dehydrogenases
Isomerases
hydroxysteroid dehydrogenases
Testosterone

Keywords

  • Anti-Müllerian hormone
  • Cryptorchid
  • Horse
  • Sertoli cell
  • Steroidogenesis
  • Testis

ASJC Scopus subject areas

  • Equine

Cite this

Expression of anti-Müllerian hormone, CDKN1B, connexin 43, androgen receptor and steroidogenic enzymes in the equine cryptorchid testis. / Almeida, J.; Conley, Alan J; Ball, B. A.

In: Equine Veterinary Journal, Vol. 45, No. 5, 09.2013, p. 538-545.

Research output: Contribution to journalArticle

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abstract = "Reasons for performing study: Cryptorchidism affects 2-8{\%} of male horses and the affected testis undergoes a disruption of normal spermatogenesis. The underlying molecular changes are poorly understood in the cryptorchid equine testis. Objectives: Compare the expression of anti-M{\"u}llerian hormone (AMH), anti-M{\"u}llerian hormone receptor (AMHR2), androgen receptor (AR), cyclin kinase inhibitor (CDKN1B), connexin 43 (Cx43), 3β hydroxysteroid dehydrogenase/Δ5-Δ4- isomerase (3βHSD), P450c17 hydroxylase/lyase (P450c17) and cytochrome P450 aromatase (P450arom) in the undescended testis of cryptorchid stallions with that of normal stallions. Methods: Undescended, abdominal testes from four cryptorchid stallions between 2 and 3 years of age were collected during routine castrations along with normally descended testes from normal stallions between 2 and 3 years of age (n = 7). Samples were analysed by immunohistochemistry and quantitative real-time PCR. Results: Cryptorchid testes had increased AMH and AMHR2 immunolabelling when compared with normal testes, which indicates failure of maturation of Sertoli cells and/or lack of testosterone suppression. Failure of Sertoli cell maturation in the cryptorchid testis may also be attributed to AR abnormalities and/or a consequence of lack of testosterone suppression due to decreased 3βHSD. Cyclin-dependent kinase (CDKN1B) was not expressed in Sertoli cells of cryptorchid testes suggesting that Sertoli cells are still proliferating, which is also a characteristic of the immature testis. In addition, Cx43 expression is decreased in the cryptorchid testis, indicating a disruption in intercellular communication. Conclusions: Undescended testes of cryptorchid horses present characteristics of immaturity suggesting that the failure of Sertoli cell maturation may be a consequence of cryptorchidism. Potential relevance: This study provides a better understanding of the effect of cryptorchidism on testicular function in stallions.",
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