Expression of anti-Müllerian hormone (AMH) in the equine testis

B. A. Ball, Alan J Conley, S. A. Grundy, K. Sabeur, Irwin Liu

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Anti-Müllerian hormone (AMH) induces regression of Müllerian ducts during male fetal development; in the human male, it is expressed in Sertoli cells during fetal development (and through puberty). The objective was to characterize expression of AMH in the fetal, neonatal, prepubertal, and adult equine testis, as well as in equine cryptorchid testes, in select testicular neoplasms, and in intersex gonads, based upon immunohistochemistry (IHC). Testes were removed from equine fetuses at 5.5, 10, and 11 months of gestation, at 12 months of age, and from adult stallions. In addition, cryptorchid testes, testis tumors (teratomas, seminomas, Sertoli cell tumors), and male intersex gonads were examined by IHC for expression of AMH using a goat polyclonal primary antibody (α-AMH) directed against a C-terminal peptide antigen from human AMH. Immunolabeling with α-AMH was localized to Sertoli cells within the developing seminiferous tubules of fetal, neonatal and prepubertal equine testes, with no expression detected in Sertoli cells from normal adult equine testes. Furthermore, expression was detected in cryptorchid testes (in animals up to 3-4 years of age) and in Sertoli cell tumors and male intersex gonads. In conclusion, AMH was strongly expressed by Sertoli cells in fetal, neonatal and prepubertal equine testes, but not in normal adult testes. That AMH was expressed in cryptorchid testes may provide a useful biomarker for detection of cryptorchid testes, as well as for immunohistochemical characterization of testicular tumors and intersex gonads in the horse.

Original languageEnglish (US)
Pages (from-to)624-631
Number of pages8
JournalTheriogenology
Volume69
Issue number5
DOIs
StatePublished - Mar 15 2008

Fingerprint

Horses
Testis
testes
hormones
Hormones
horses
Sertoli cells
Sertoli Cells
Gonads
gonads
Sertoli Cell Tumor
neoplasms
Testicular Neoplasms
fetal development
Fetal Development
immunohistochemistry
Immunohistochemistry
Seminoma
Seminiferous Tubules
seminiferous tubules

Keywords

  • Anti-Müllerian hormone
  • Cryptorchidism
  • Equine
  • Sertoli cell
  • Testis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Expression of anti-Müllerian hormone (AMH) in the equine testis. / Ball, B. A.; Conley, Alan J; Grundy, S. A.; Sabeur, K.; Liu, Irwin.

In: Theriogenology, Vol. 69, No. 5, 15.03.2008, p. 624-631.

Research output: Contribution to journalArticle

Ball, B. A. ; Conley, Alan J ; Grundy, S. A. ; Sabeur, K. ; Liu, Irwin. / Expression of anti-Müllerian hormone (AMH) in the equine testis. In: Theriogenology. 2008 ; Vol. 69, No. 5. pp. 624-631.
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