Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries

B. A. Ball, Alan J Conley, D. T. MacLaughlin, S. A. Grundy, K. Sabeur, Irwin Liu

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

Anti-Müllerian hormone (AMH), also known as Müllerian inhibiting substance (MIS), is expressed by granulosa cells in females of many mammalian species, and circulating AMH concentrations have been used to monitor granulosa-cell tumors (GCT) in women. The objective was to characterize expression of AMH in equine GCT, and in normal equine ovaries, based upon immunohistochemistry (IHC), using a polyclonal primary antibody directed against human AMH. Equine GCT (n = 27) and normal equine ovaries (n = 10) were examined by IHC. In addition, sera from four mares with GCT were characterized for AMH bioactivity, based upon suppression of Müllerian duct development in the fetal rat. Immunolabeling with α-AMH was localized to granulosa cells in equine GCT, as well as within antral follicles in normal ovaries. Expression of AMH first appeared in granulosa cells of small growing follicles and was most intense in small antral follicles; large antral or atretic follicles had reduced immunolabeling. Omission of the primary antibody or incubation of the primary antibody with the corresponding blocking peptide eliminated immunolabeling of granulosa cells in GCT and in normal antral follicles, confirming the specificity of the immunolabel. Sera from mares with GCT had increased AMH bioactivity compared to control sera. In conclusion, AMH was strongly expressed by granulosa cells in equine GCT and in normal antral follicles. Therefore, anti-Müllerian hormone may be a useful biomarker for detection of GCT in the horse.

Original languageEnglish (US)
Pages (from-to)968-977
Number of pages10
JournalTheriogenology
Volume70
Issue number6
DOIs
StatePublished - Oct 1 2008

Keywords

  • Anti-Müllerian hormone
  • Equine
  • Granulosa-cell tumor
  • Ovarian follicle
  • Ovary

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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