Expression of active human immunodeficiency virus reverse transcriptase in Saccharomyces cerevisiae

Philip J. Barr, Michael D. Power, Chun Ting Lee-Ng, Helen L. Gibson, Paul A Luciw

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The reverse transcriptase (RT) domain of the human immunodeficiency virus (HIV) pol gene was expressed in yeast. Purification and analysis of the recombinant protein indicated that yeast post-translational processing of RT was similar, if not identical, to that which had previously been observed in HIV infected lymphoid cells. In addition, recombinant RT was enzymatically active and could be inhibited by several anti-retroviral agents. These studies indicate that HIV RT expressed in yeast will be useful for the in vitro analysis of further potential inhibitors of this important enzyme.

Original languageEnglish (US)
Pages (from-to)486-489
Number of pages4
JournalBio/Technology
Volume5
Issue number5
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

HIV Reverse Transcriptase
RNA-Directed DNA Polymerase
Viruses
Yeast
Saccharomyces cerevisiae
Yeasts
pol Genes
Anti-Retroviral Agents
Recombinant proteins
Enzyme Inhibitors
Recombinant Proteins
Purification
Enzymes
Genes
HIV
Lymphocytes
Processing

ASJC Scopus subject areas

  • Biotechnology

Cite this

Expression of active human immunodeficiency virus reverse transcriptase in Saccharomyces cerevisiae. / Barr, Philip J.; Power, Michael D.; Lee-Ng, Chun Ting; Gibson, Helen L.; Luciw, Paul A.

In: Bio/Technology, Vol. 5, No. 5, 1987, p. 486-489.

Research output: Contribution to journalArticle

Barr, Philip J. ; Power, Michael D. ; Lee-Ng, Chun Ting ; Gibson, Helen L. ; Luciw, Paul A. / Expression of active human immunodeficiency virus reverse transcriptase in Saccharomyces cerevisiae. In: Bio/Technology. 1987 ; Vol. 5, No. 5. pp. 486-489.
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