Expression of a transforming gene (E5) of bovine papillomavirus in sarcoids obtained from horses

Elizabeth A. Carr, Alain P Theon, Bruce R. Madewell, Margaret E. Hitchcock, Richard Schlegel, John T. Schiller

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Objective - To determine expression of a transforming gene (E5) of bovine papillomavirus in sarcoids, other tumors, and normal skin samples collected from horses with and without sarcoids. Sample Population - 23 sarcoids and 6 samples of normal skin obtained from 16 horses with sarcoids, 2 samples of normal skin and 2 papillomas obtained from horses without sarcoids, and 1 papilloma obtained from a cow. Procedure - Protein was extracted from tissue samples collected from horses and incubated with agarose beads covalently coupled to Staphylococcus aureus protein A and an anti-E5 polyclonal antibody. Following incubation, proteins were eluted from the beads and electrophoresed on a 14% polyacrylamide gel and transferred to a polyvinylidene difluoride membrane. The E5 protein was detected by use of western blot analysis, using a chemiluminescence detection system. Results - All 23 sarcoids had positive results for expression of E5 protein. Quantity of viral protein appeared to vary among sarcoids. All other tissues examined had negative results for E5 protein. Highest expression for E5 protein was observed in biologically aggressive fibroblastic variants of sarcoids, compared with expression in quiescent tumors. Conclusions and Clinical Relevance - This study documented that activation and expression of the E5 gene is evident in sarcoids obtained from horses. These data support the conclusion that infection with bovine papillomavirus is important in the initiation or progression of sarcoids in horses. Treatment strategies designed to increase immune recognition of virally infected cells are warranted.

Original languageEnglish (US)
Pages (from-to)1212-1217
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume62
Issue number8
StatePublished - Aug 2001

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Bovine papillomavirus
sarcoid
Oncogenes
Horses
horses
Proteins
genes
Papilloma
Skin
papilloma
proteins
Staphylococcal Protein A
Viral Proteins
Luminescence
sampling
Sepharose
protein synthesis
Staphylococcus aureus
Neoplasms
Western Blotting

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Carr, E. A., Theon, A. P., Madewell, B. R., Hitchcock, M. E., Schlegel, R., & Schiller, J. T. (2001). Expression of a transforming gene (E5) of bovine papillomavirus in sarcoids obtained from horses. American Journal of Veterinary Research, 62(8), 1212-1217.

Expression of a transforming gene (E5) of bovine papillomavirus in sarcoids obtained from horses. / Carr, Elizabeth A.; Theon, Alain P; Madewell, Bruce R.; Hitchcock, Margaret E.; Schlegel, Richard; Schiller, John T.

In: American Journal of Veterinary Research, Vol. 62, No. 8, 08.2001, p. 1212-1217.

Research output: Contribution to journalArticle

Carr, EA, Theon, AP, Madewell, BR, Hitchcock, ME, Schlegel, R & Schiller, JT 2001, 'Expression of a transforming gene (E5) of bovine papillomavirus in sarcoids obtained from horses', American Journal of Veterinary Research, vol. 62, no. 8, pp. 1212-1217.
Carr, Elizabeth A. ; Theon, Alain P ; Madewell, Bruce R. ; Hitchcock, Margaret E. ; Schlegel, Richard ; Schiller, John T. / Expression of a transforming gene (E5) of bovine papillomavirus in sarcoids obtained from horses. In: American Journal of Veterinary Research. 2001 ; Vol. 62, No. 8. pp. 1212-1217.
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