Expression of a stable articular cartilage phenotype without evidence of hypertrophy by adult human articular chondrocytes in vitro

F. Binette, D. P. McQuaid, Dominik R Haudenschild, P. C. Yaeger, J. M. McPherson, R. Tubo

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Chondrocytes that were isolated from adult human articular cartilage changed phenotype during monolayer tissue culture, as characterized by a fibroblastic morphology and cellular proliferation. Increased proliferation was accompanied by downregulation of the cartilage-specific extracellular matrix proteoglycan, aggrecan, by cessation of type-II collagen expression, and by upregulation of type-I collagen and versican. This phenomenon observed in monolayer was reversible after the transfer of cells to a suspension culture system. The transfer of chondrocytes to suspension culture in alginate beads resulted in the rapid upregulation of aggrecan and type-II collagen and the downregulation of expression of versican and type-I collagen. Type-X collagen and osteopontin, markers of chondrocyte hypertrophy and commitment to endochondral ossification, were not expressed by adult articular chondrocytes cultured in alginate, even after 5 months. In contrast, type-X collagen was expressed within 2 weeks in a population of cells derived from a fetal growth plate. The inability of adult articular chondrocytes to express markers of chondrocyte hypertrophy has underscored the fundamental distinction between the differentiation pathways that lead to articular cartilage or to bone. Adult articular chondrocytes expressed only hyaline articular cartilage markers without evidence of hypertrophy.

Original languageEnglish (US)
Pages (from-to)207-216
Number of pages10
JournalJournal of Orthopaedic Research
Volume16
Issue number2
DOIs
StatePublished - Mar 1998
Externally publishedYes

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Articular Cartilage
Chondrocytes
Hypertrophy
Joints
Phenotype
Versicans
Collagen Type X
Aggrecans
Collagen Type II
Collagen Type I
Suspensions
Up-Regulation
Down-Regulation
Hyaline Cartilage
Osteopontin
Growth Plate
Proteoglycans
Fetal Development
In Vitro Techniques
Osteogenesis

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Expression of a stable articular cartilage phenotype without evidence of hypertrophy by adult human articular chondrocytes in vitro. / Binette, F.; McQuaid, D. P.; Haudenschild, Dominik R; Yaeger, P. C.; McPherson, J. M.; Tubo, R.

In: Journal of Orthopaedic Research, Vol. 16, No. 2, 03.1998, p. 207-216.

Research output: Contribution to journalArticle

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