Expression of a recombinant murine IgE in transfected myeloma cells

C. A. Gritzmacher, Fu-Tong Liu

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

We constructed a recombinant gene encoding an immunologlobulin (Ig) heavy chain consisting of the variable region from the phosphorylcholine (PC)-specific secreting myeloma MOPC167 and the ε constant region from SJL mice. This gene, cloned into the shuttle vector pSV2gpt, was transfected into J558L myeloma cells, and stable transformants that expressed the ε gene were cloned. The IgE heavy chain in these transformants is associated with the endogenous λ light chain and is secreted as an intact IgE molecule. However, the secreted IgE does not bind to PC conjugated to bovine serum albumin (PC-BSA). The MOPC167 κ chain gene was cloned into the shuttle vector pSV2neo and was transfected into the ε heavy-chain transformant. Stable transformants were cloned that expressed both the ε heavy chain and the κ light chain. IgE secreted from such a transformant was shown to bind to PC-BSA. Both types of secreted recombinant IgE bound to rat basophilic leukemia (RBL) cells, but only the IgE produced by the cell line transformed with the MOPC167 κ gene could be cross-linked with PC-BSA to cause serotonin release.

Original languageEnglish (US)
Pages (from-to)324-329
Number of pages6
JournalJournal of Immunology
Volume138
Issue number1
StatePublished - 1987

ASJC Scopus subject areas

  • Immunology

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    Gritzmacher, C. A., & Liu, F-T. (1987). Expression of a recombinant murine IgE in transfected myeloma cells. Journal of Immunology, 138(1), 324-329.