Expression of a recombinant murine IgE in transfected myeloma cells

C. A. Gritzmacher, Fu-Tong Liu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We constructed a recombinant gene encoding an immunologlobulin (Ig) heavy chain consisting of the variable region from the phosphorylcholine (PC)-specific secreting myeloma MOPC167 and the ε constant region from SJL mice. This gene, cloned into the shuttle vector pSV2gpt, was transfected into J558L myeloma cells, and stable transformants that expressed the ε gene were cloned. The IgE heavy chain in these transformants is associated with the endogenous λ light chain and is secreted as an intact IgE molecule. However, the secreted IgE does not bind to PC conjugated to bovine serum albumin (PC-BSA). The MOPC167 κ chain gene was cloned into the shuttle vector pSV2neo and was transfected into the ε heavy-chain transformant. Stable transformants were cloned that expressed both the ε heavy chain and the κ light chain. IgE secreted from such a transformant was shown to bind to PC-BSA. Both types of secreted recombinant IgE bound to rat basophilic leukemia (RBL) cells, but only the IgE produced by the cell line transformed with the MOPC167 κ gene could be cross-linked with PC-BSA to cause serotonin release.

Original languageEnglish (US)
Pages (from-to)324-329
Number of pages6
JournalJournal of Immunology
Volume138
Issue number1
StatePublished - 1987

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Immunoglobulin E
Phosphorylcholine
Genetic Vectors
Genes
Light
Transformed Cell Line
Bovine Serum Albumin
Serotonin
Leukemia

ASJC Scopus subject areas

  • Immunology

Cite this

Gritzmacher, C. A., & Liu, F-T. (1987). Expression of a recombinant murine IgE in transfected myeloma cells. Journal of Immunology, 138(1), 324-329.

Expression of a recombinant murine IgE in transfected myeloma cells. / Gritzmacher, C. A.; Liu, Fu-Tong.

In: Journal of Immunology, Vol. 138, No. 1, 1987, p. 324-329.

Research output: Contribution to journalArticle

Gritzmacher, CA & Liu, F-T 1987, 'Expression of a recombinant murine IgE in transfected myeloma cells', Journal of Immunology, vol. 138, no. 1, pp. 324-329.
Gritzmacher, C. A. ; Liu, Fu-Tong. / Expression of a recombinant murine IgE in transfected myeloma cells. In: Journal of Immunology. 1987 ; Vol. 138, No. 1. pp. 324-329.
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