Abstract
We constructed a recombinant gene encoding an immunologlobulin (Ig) heavy chain consisting of the variable region from the phosphorylcholine (PC)-specific secreting myeloma MOPC167 and the ε constant region from SJL mice. This gene, cloned into the shuttle vector pSV2gpt, was transfected into J558L myeloma cells, and stable transformants that expressed the ε gene were cloned. The IgE heavy chain in these transformants is associated with the endogenous λ light chain and is secreted as an intact IgE molecule. However, the secreted IgE does not bind to PC conjugated to bovine serum albumin (PC-BSA). The MOPC167 κ chain gene was cloned into the shuttle vector pSV2neo and was transfected into the ε heavy-chain transformant. Stable transformants were cloned that expressed both the ε heavy chain and the κ light chain. IgE secreted from such a transformant was shown to bind to PC-BSA. Both types of secreted recombinant IgE bound to rat basophilic leukemia (RBL) cells, but only the IgE produced by the cell line transformed with the MOPC167 κ gene could be cross-linked with PC-BSA to cause serotonin release.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 324-329 |
| Number of pages | 6 |
| Journal | Journal of Immunology |
| Volume | 138 |
| Issue number | 1 |
| State | Published - 1987 |
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ASJC Scopus subject areas
- Immunology
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Expression of a recombinant murine IgE in transfected myeloma cells. / Gritzmacher, C. A.; Liu, Fu-Tong.
In: Journal of Immunology, Vol. 138, No. 1, 1987, p. 324-329.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of a recombinant murine IgE in transfected myeloma cells
AU - Gritzmacher, C. A.
AU - Liu, Fu-Tong
PY - 1987
Y1 - 1987
N2 - We constructed a recombinant gene encoding an immunologlobulin (Ig) heavy chain consisting of the variable region from the phosphorylcholine (PC)-specific secreting myeloma MOPC167 and the ε constant region from SJL mice. This gene, cloned into the shuttle vector pSV2gpt, was transfected into J558L myeloma cells, and stable transformants that expressed the ε gene were cloned. The IgE heavy chain in these transformants is associated with the endogenous λ light chain and is secreted as an intact IgE molecule. However, the secreted IgE does not bind to PC conjugated to bovine serum albumin (PC-BSA). The MOPC167 κ chain gene was cloned into the shuttle vector pSV2neo and was transfected into the ε heavy-chain transformant. Stable transformants were cloned that expressed both the ε heavy chain and the κ light chain. IgE secreted from such a transformant was shown to bind to PC-BSA. Both types of secreted recombinant IgE bound to rat basophilic leukemia (RBL) cells, but only the IgE produced by the cell line transformed with the MOPC167 κ gene could be cross-linked with PC-BSA to cause serotonin release.
AB - We constructed a recombinant gene encoding an immunologlobulin (Ig) heavy chain consisting of the variable region from the phosphorylcholine (PC)-specific secreting myeloma MOPC167 and the ε constant region from SJL mice. This gene, cloned into the shuttle vector pSV2gpt, was transfected into J558L myeloma cells, and stable transformants that expressed the ε gene were cloned. The IgE heavy chain in these transformants is associated with the endogenous λ light chain and is secreted as an intact IgE molecule. However, the secreted IgE does not bind to PC conjugated to bovine serum albumin (PC-BSA). The MOPC167 κ chain gene was cloned into the shuttle vector pSV2neo and was transfected into the ε heavy-chain transformant. Stable transformants were cloned that expressed both the ε heavy chain and the κ light chain. IgE secreted from such a transformant was shown to bind to PC-BSA. Both types of secreted recombinant IgE bound to rat basophilic leukemia (RBL) cells, but only the IgE produced by the cell line transformed with the MOPC167 κ gene could be cross-linked with PC-BSA to cause serotonin release.
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UR - http://www.scopus.com/inward/citedby.url?scp=0023271213&partnerID=8YFLogxK
M3 - Article
C2 - 2431059
AN - SCOPUS:0023271213
VL - 138
SP - 324
EP - 329
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 1
ER -