Abstract
The relative abundance of the nonstructural protein NS1 in bluetongue virus (BTV)-infected cells, the existence of NS1 in the BTV particles and the highly conserved NS1 gene among BTV serotypes indicate the diagnostic potential of using NS1 in detecting BTV infections. In this study a NS1 gene was expressed with the T7 RNA polymerase expression system to produce a full-length NS1 protein. Sheep anti-NS1 antibodies were raised with the E. coli-produced NS1 and used to show that the NS1 proteins of the five BTV serotypes in the United States were immunologically indistinguishable.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 994-1001 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 180 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 31 1991 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Expression of a full-length nonstructural protein NS1 of bluetongue virus serotype 17 in Escherichia coli. / He, Xiaosong; Wang, Lin Fa; Doi, Roy H.; Maia, Maurecio; Osburn, Bennie; Chuang, Ronald Y.
In: Biochemical and Biophysical Research Communications, Vol. 180, No. 2, 31.10.1991, p. 994-1001.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of a full-length nonstructural protein NS1 of bluetongue virus serotype 17 in Escherichia coli
AU - He, Xiaosong
AU - Wang, Lin Fa
AU - Doi, Roy H.
AU - Maia, Maurecio
AU - Osburn, Bennie
AU - Chuang, Ronald Y.
PY - 1991/10/31
Y1 - 1991/10/31
N2 - The relative abundance of the nonstructural protein NS1 in bluetongue virus (BTV)-infected cells, the existence of NS1 in the BTV particles and the highly conserved NS1 gene among BTV serotypes indicate the diagnostic potential of using NS1 in detecting BTV infections. In this study a NS1 gene was expressed with the T7 RNA polymerase expression system to produce a full-length NS1 protein. Sheep anti-NS1 antibodies were raised with the E. coli-produced NS1 and used to show that the NS1 proteins of the five BTV serotypes in the United States were immunologically indistinguishable.
AB - The relative abundance of the nonstructural protein NS1 in bluetongue virus (BTV)-infected cells, the existence of NS1 in the BTV particles and the highly conserved NS1 gene among BTV serotypes indicate the diagnostic potential of using NS1 in detecting BTV infections. In this study a NS1 gene was expressed with the T7 RNA polymerase expression system to produce a full-length NS1 protein. Sheep anti-NS1 antibodies were raised with the E. coli-produced NS1 and used to show that the NS1 proteins of the five BTV serotypes in the United States were immunologically indistinguishable.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025788225&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)81164-7
DO - 10.1016/S0006-291X(05)81164-7
M3 - Article
C2 - 1659409
AN - SCOPUS:0025788225
VL - 180
SP - 994
EP - 1001
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -