Expression of a full-length nonstructural protein NS1 of bluetongue virus serotype 17 in Escherichia coli

Xiaosong He; Lin Fa Wang; Roy H. Doi; Maurecio Maia; Bennie Osburn; Ronald Y. Chuang

doi:10.1016/S0006-291X(05)81164-7

Expression of a full-length nonstructural protein NS1 of bluetongue virus serotype 17 in Escherichia coli

Xiaosong He, Lin Fa Wang, Roy H. Doi, Maurecio Maia, Bennie Osburn, Ronald Y. Chuang

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The relative abundance of the nonstructural protein NS1 in bluetongue virus (BTV)-infected cells, the existence of NS1 in the BTV particles and the highly conserved NS1 gene among BTV serotypes indicate the diagnostic potential of using NS1 in detecting BTV infections. In this study a NS1 gene was expressed with the T7 RNA polymerase expression system to produce a full-length NS1 protein. Sheep anti-NS1 antibodies were raised with the E. coli-produced NS1 and used to show that the NS1 proteins of the five BTV serotypes in the United States were immunologically indistinguishable.

Original language	English (US)
Pages (from-to)	994-1001
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	180
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(05)81164-7
State	Published - Oct 31 1991

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Expression of a full-length nonstructural protein NS1 of bluetongue virus serotype 17 in Escherichia coli",

abstract = "The relative abundance of the nonstructural protein NS1 in bluetongue virus (BTV)-infected cells, the existence of NS1 in the BTV particles and the highly conserved NS1 gene among BTV serotypes indicate the diagnostic potential of using NS1 in detecting BTV infections. In this study a NS1 gene was expressed with the T7 RNA polymerase expression system to produce a full-length NS1 protein. Sheep anti-NS1 antibodies were raised with the E. coli-produced NS1 and used to show that the NS1 proteins of the five BTV serotypes in the United States were immunologically indistinguishable.",

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AU - He, Xiaosong

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AU - Osburn, Bennie

AU - Chuang, Ronald Y.

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AB - The relative abundance of the nonstructural protein NS1 in bluetongue virus (BTV)-infected cells, the existence of NS1 in the BTV particles and the highly conserved NS1 gene among BTV serotypes indicate the diagnostic potential of using NS1 in detecting BTV infections. In this study a NS1 gene was expressed with the T7 RNA polymerase expression system to produce a full-length NS1 protein. Sheep anti-NS1 antibodies were raised with the E. coli-produced NS1 and used to show that the NS1 proteins of the five BTV serotypes in the United States were immunologically indistinguishable.

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Expression of a full-length nonstructural protein NS1 of bluetongue virus serotype 17 in Escherichia coli

Abstract

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