Expression of 5-HT3 receptors by extrinsic duodenal afferents contribute to intestinal inhibition of gastric emptying

Helen E Raybould, Jorg Glatzle, Carla Robin, James H. Meyer, Thomas Phan, Helen Wong, Catia Sternini

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Intestinal perfusion with carbohydrates inhibits gastric emptying via vagal and spinal capsaicin-sensitive afferent pathways. The aim of the present study was to determine the role of 1) 5-hydroxytryptamine (5-HT)3 receptors (5-HT3R) in mediating glucose-induced inhibition of gastric emptying and 2) 5-HT3R expression in vagal and spinal afferents in innervating the duodenum. In awake rats fitted with gastric and duodenal cannulas, perfusion of the duodenum with glucose (50 and 100 mg) inhibited gastric emptying. Intestinal perfusion of mannitol inhibited gastric emptying only at the highest concentration (990 mosm/kgH2O). Pretreatment with the 5-HT3R antagonist tropisetron abolished both glucose- and mannitol-induced inhibition of gastric emptying. Retrograde labeling of visceral afferents by injection of dextran-conjugated Texas Red into the duodenal wall was used to identify extrinsic primary afferents. Immunoreactivity for 5-HT3R, visualized with an antibody directed to the COOH terminus of the rat 5-HT3R, was found in >80% of duodenal vagal and spinal afferents. These results show that duodenal extrinsic afferents express 5-HT3R and that the receptor mediates specific glucose-induced inhibition of gastric emptying. These findings support the hypothesis that enterochromaffin cells in the intestinal mucosa release 5-HT in response to glucose, which activates 5-HT3R on afferent nerve terminals to evoke reflex changes in gastric motility. The primary glucose sensors of the intestine may be mucosal enterochromaffin cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume284
Issue number3 47-3
StatePublished - Mar 1 2003

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Receptors, Serotonin, 5-HT3
Gastric Emptying
Serotonin Receptors
Glucose
Enterochromaffin Cells
tropisetron
Perfusion
Mannitol
Duodenum
Stomach
Visceral Afferents
Serotonin 5-HT2 Receptors
Afferent Pathways
Capsaicin
Intestinal Mucosa
Dextrans
Intestines
Reflex
Inhibition (Psychology)
Serotonin

Keywords

  • 5-hydroxytryptamine
  • Carbohydrate
  • Enterochromaffin cells
  • Glucose
  • Monosaccharide

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Expression of 5-HT3 receptors by extrinsic duodenal afferents contribute to intestinal inhibition of gastric emptying. / Raybould, Helen E; Glatzle, Jorg; Robin, Carla; Meyer, James H.; Phan, Thomas; Wong, Helen; Sternini, Catia.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 284, No. 3 47-3, 01.03.2003.

Research output: Contribution to journalArticle

Raybould, Helen E ; Glatzle, Jorg ; Robin, Carla ; Meyer, James H. ; Phan, Thomas ; Wong, Helen ; Sternini, Catia. / Expression of 5-HT3 receptors by extrinsic duodenal afferents contribute to intestinal inhibition of gastric emptying. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2003 ; Vol. 284, No. 3 47-3.
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