Expression, genomic structure and mapping of the thymus specific protease Prss16: A candidate gene for insulin dependent diabetes mellitus susceptibility

Saijai Cheunsuk, Rachel Sparks, Janice K. Noveroske, Tom Hsu, Monica J. Justice, M. Eric Gershwin, Jeffrey R. Gruen, Christopher Bowlus

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

PRSS16 is a serine protease specifically expressed by epithelial cells in the thymic cortex. The human gene is encoded on 6p21.3-p22 where recent linkage analysis has identified an association with insulin dependent diabetes mellitus (IDDM) susceptibility independent of HLA-DR3. To further investigate its potential role in autoimmunity, we characterized the mouse orthologue, Prss16. The genomic structure of Prss16 shows conservation with the human gene in size, number of exons and chromosomal location. Mapping of Prss16 places it on mouse chromosome 13 centromeric of the satin locus. This region is comparable to the PRSS16 region on human chromosome 6 and has also been linked to quantitative trait locus for IDDM in the nonobese diabetic mouse. Similar to the human gene, Prss16 expression is highly specific in the mouse with expression limited to the cortical thymic epithelium. Notably, embryonic expression coincides with population of the thymic anlage with T-cell precursors and initiation of T-cell development. We also show that NOD and New Zealand Black mice, which have a disrupted thymic architecture and autoimmune phenotype, have lower levels of Prss16 expression compared to C57BL/6 mice. These findings support the role of Prss16 in T-cell development and susceptibility to autoimmunity in the mouse.

Original languageEnglish (US)
Pages (from-to)311-316
Number of pages6
JournalJournal of Autoimmunity
Volume18
Issue number4
DOIs
StatePublished - Jun 2002

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Type 1 Diabetes Mellitus
Thymus Gland
Peptide Hydrolases
Genes
Autoimmunity
HLA-DR3 Antigen
T-Lymphoid Precursor Cells
T-Lymphocytes
Chromosomes, Human, Pair 13
Inbred NOD Mouse
Chromosomes, Human, Pair 6
Quantitative Trait Loci
Human Chromosomes
Serine Proteases
New Zealand
Inbred C57BL Mouse
Exons
Epithelium
Epithelial Cells
Phenotype

Keywords

  • Autoimmunity
  • Gene expression
  • Prss16
  • Thymus

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Expression, genomic structure and mapping of the thymus specific protease Prss16 : A candidate gene for insulin dependent diabetes mellitus susceptibility. / Cheunsuk, Saijai; Sparks, Rachel; Noveroske, Janice K.; Hsu, Tom; Justice, Monica J.; Gershwin, M. Eric; Gruen, Jeffrey R.; Bowlus, Christopher.

In: Journal of Autoimmunity, Vol. 18, No. 4, 06.2002, p. 311-316.

Research output: Contribution to journalArticle

Cheunsuk, Saijai ; Sparks, Rachel ; Noveroske, Janice K. ; Hsu, Tom ; Justice, Monica J. ; Gershwin, M. Eric ; Gruen, Jeffrey R. ; Bowlus, Christopher. / Expression, genomic structure and mapping of the thymus specific protease Prss16 : A candidate gene for insulin dependent diabetes mellitus susceptibility. In: Journal of Autoimmunity. 2002 ; Vol. 18, No. 4. pp. 311-316.
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