Abstract
The expression of outer surface protein C (OspC) was determined for North American Borrelia burgdorferi isolates HB19, DN127c19-2, 25015 and both low and high culture passage B31. A monoclonal antibody detected the presence of OspC protein in only two isolates, while polyclonal antiserum identified this protein in all five isolates. The ospC gene was cloned and sequenced for isolates HB19, DN127c19-2 and 25015, and compared with the published ospC sequences of other Lyme disease spirochetes. Bothe the nucleotide and amino acid sequences were found to vary as much among isolates from the same geographic area as between isolates of different species.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 367-372 |
| Number of pages | 6 |
| Journal | FEMS Microbiology Letters |
| Volume | 124 |
| Issue number | 3 |
| State | Published - Dec 15 1994 |
| Externally published | Yes |
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Keywords
- Borrelia burgdorferi
- Lyme disease
- OspC
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
Expression and sequence of outer surface protein C among North American isolates of Borrelia burgdorferi. / Stevenson, Brian; Barthold, Stephen W.
In: FEMS Microbiology Letters, Vol. 124, No. 3, 15.12.1994, p. 367-372.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and sequence of outer surface protein C among North American isolates of Borrelia burgdorferi
AU - Stevenson, Brian
AU - Barthold, Stephen W
PY - 1994/12/15
Y1 - 1994/12/15
N2 - The expression of outer surface protein C (OspC) was determined for North American Borrelia burgdorferi isolates HB19, DN127c19-2, 25015 and both low and high culture passage B31. A monoclonal antibody detected the presence of OspC protein in only two isolates, while polyclonal antiserum identified this protein in all five isolates. The ospC gene was cloned and sequenced for isolates HB19, DN127c19-2 and 25015, and compared with the published ospC sequences of other Lyme disease spirochetes. Bothe the nucleotide and amino acid sequences were found to vary as much among isolates from the same geographic area as between isolates of different species.
AB - The expression of outer surface protein C (OspC) was determined for North American Borrelia burgdorferi isolates HB19, DN127c19-2, 25015 and both low and high culture passage B31. A monoclonal antibody detected the presence of OspC protein in only two isolates, while polyclonal antiserum identified this protein in all five isolates. The ospC gene was cloned and sequenced for isolates HB19, DN127c19-2 and 25015, and compared with the published ospC sequences of other Lyme disease spirochetes. Bothe the nucleotide and amino acid sequences were found to vary as much among isolates from the same geographic area as between isolates of different species.
KW - Borrelia burgdorferi
KW - Lyme disease
KW - OspC
UR - http://www.scopus.com/inward/record.url?scp=0027999968&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027999968&partnerID=8YFLogxK
M3 - Article
C2 - 7851744
AN - SCOPUS:0027999968
VL - 124
SP - 367
EP - 372
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 3
ER -